Morphological alterations on Citrobacter freundii bacteria induced by erythrosine dye and laser light

Silva, Josmary R.; Cardoso, Gleidson; Maciel, Rafael R. G.; Souza, Nara C. de

doi:10.1007/s10103-013-1421-3

Cited by 9 publications

(6 citation statements)

References 30 publications

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“…It was demonstrated that the PS absorption spectrum increases proportionally with its concentration and differs between the two dyes. This results is in accordance with previous studies [ 12 , 22 ] and highlight the importance of taking into account the type of PS in light dose calculations, which are frequently neglected in most of the studies evaluating aPDI effectiveness [ 23 , 24 , 25 ]. A reasonable explation for this finding is provided by the equations demonstrated in the Supplementary Material , where the light dose absorbed (D Abs ) by each photosensitizer is obtained by relating the illumination time ( t ), the irradiated area (A) and the absorbed power (P Abs ) Equation (S11).…”

Section: Discussionsupporting

confidence: 92%

Antimicrobial Photodynamic Inactivation Mediated by Rose Bengal and Erythrosine Is Effective in the Control of Food-Related Bacteria in Planktonic and Biofilm States

et al. 2018

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The thermal and chemical-based methods applied for microbial control in the food industry are not always environmentally friendly and may change the nutritional and organoleptic characteristics of the final products. Moreover, the efficacy of sanitizing agents may be reduced when microbial cells are enclosed in biofilms. The objective of this study was to investigate the effect of photodynamic inactivation, using two xanthene dyes (rose bengal and erythrosine) as photosensitizing agents and green LED as a light source, against Staphylococcus aureus, Listeria innocua, Enterococcus hirae and Escherichia coli in both planktonic and biofilm states. Both photosensitizing agents were able to control planktonic cells of all bacteria tested. The treatments altered the physicochemical properties of cells surface and also induced potassium leakage, indicating damage of cell membranes. Although higher concentrations of the photosensitizing agents (ranging from 0.01 to 50.0 μmol/L) were needed to be applied, the culturability of biofilm cells was reduced to undetectable levels. This finding was confirmed by the live/dead staining, where propidium iodide-labeled bacteria numbers reached up to 100%. The overall results demonstrated that photoinactivation by rose bengal and erythrosine may be a powerful candidate for the control of planktonic cells and biofilms in the food sector.

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Section: Discussionsupporting

confidence: 92%

Antimicrobial Photodynamic Inactivation Mediated by Rose Bengal and Erythrosine Is Effective in the Control of Food-Related Bacteria in Planktonic and Biofilm States

et al. 2018

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“…Those authors concluded that erythrosine at 22 µM in the presence of a blue light and infrared diode laser could be considered as a potential approach of PDI against Gram-negative periodontal pathogenic species. Nagata et al [36], in a review of PDI for dental caries, reported that erythrosine is the most hydrophilic photosensitizer and the Gram-positive bacteria allow better penetration of this dye, but Habiboallah et al [35] and Silva et al [37] showed good results with Gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic inactivation of planktonic cultures of Streptococcus mutans using erythrosine irradiated by LED

Esper

Barbosa

Alvarenga

et al. 2020

BDS

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Objective: The aim of this in vitro study was to evaluate the efficacy of photodynamic inactivation (PDI) with erythrosine (E), using a light-emitting diode (LED) on planktonic cultures of Streptococcus mutans. Material and Methods: A Streptococcus mutans strain (UA 159) was used to prepare the suspensions containing 107 cells/mL, which was tested under different experimental conditions: a) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); b) LED irradiation only (P-L+); c) treatment with erythrosine only (E+L-); and d) no LED irradiation or photosensitizer (P) treatment, which served as a control group (P-L-). After treatment, strains were seeded onto MSBS agar for determination of the number of colony-forming units (CFU/mL). Results: The results were submitted to analysis of variance and the Tukey test (p < 0.05). No reduction in the number of CFU/mL was observed in the treatment group with erythrosine (E+L+) when compared to the control (P-L-). Conclusion: PDI using erythrosine did not reduce the number of CFUs per millimeter within the parameters in this study.KEYWORDSErythrosine; Decay; Photodynamic inactivation; Light-emitting diode.

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“…It can be clearly observed that the surface of the membrane tested was completely covered by deposit in which the Citrobacter freundii cells can be seen. As it has been described in [81] they are rod shaped and typically range from 1 to 5 µm in length.…”

Section: Filtration Of Fermentation Broths With Citrobacer Freundii Bacteriamentioning

confidence: 95%

Comparison of Polypropylene and Ceramic Microfiltration Membranes Applied for Separation of 1,3-PD Fermentation Broths and Saccharomyces cerevisiae Yeast Suspensions

Tomczak

Gryta

2021

Membranes

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In recent years, microfiltration (MF) has gained great interest as an excellent technique for clarification of biological suspensions. This paper addresses a direct comparison of efficiency, performance and susceptibility to cleaning of the ceramic and polymeric MF membranes applied for purification of 1,3-propanediol (1,3-PD) fermentation broths and suspensions of yeast Saccharomyces cerevisiae. For this purpose, ceramic, titanium dioxide (TiO2) based membranes and polypropylene (PP) membranes were used. It has been found that both TiO2 and PP membranes provide sterile permeate during filtration of 1,3-PD broths. However, the ceramic membrane, due to the smaller pore diameter, allowed obtaining a better quality permeate. All the membranes used were highly susceptible to fouling with the components of the clarified broths and yeast suspensions. The significant impact of the feed flow velocity and fermentation broth composition on the relative permeate flux has been demonstrated. Suitable cleaning agents with selected concentration and duration of action effectively cleaned the ceramic membrane. In turn, the use of aggressive cleaning solutions led to degradation of the PP membranes matrix. Findings of this study add to a growing body of literature on the use of ceramic and polypropylene MF membranes for the clarification of biological suspensions.

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Morphological alterations on Citrobacter freundii bacteria induced by erythrosine dye and laser light

Cited by 9 publications

References 30 publications

Antimicrobial Photodynamic Inactivation Mediated by Rose Bengal and Erythrosine Is Effective in the Control of Food-Related Bacteria in Planktonic and Biofilm States

Antimicrobial Photodynamic Inactivation Mediated by Rose Bengal and Erythrosine Is Effective in the Control of Food-Related Bacteria in Planktonic and Biofilm States

Photodynamic inactivation of planktonic cultures of Streptococcus mutans using erythrosine irradiated by LED

Comparison of Polypropylene and Ceramic Microfiltration Membranes Applied for Separation of 1,3-PD Fermentation Broths and Saccharomyces cerevisiae Yeast Suspensions

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