Morphological Abnormalities in Tissue Culture-Derived Date Palm (Phoenix Dactylifera L.)

Al-Mazroui, H.S.; Zaid, A.; Bouhouche, Naima

doi:10.17660/actahortic.2007.736.31

Cited by 12 publications

(5 citation statements)

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“…Indeed, LoSchiavo et al (1989 showed that auxins impact global DNA methylation rates which might disturb gene expression and phenotype. Al-Mazroui et al (2007) confirmed that high amount of PGRs was the cause of somaclonal variation in date palm. Fig.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 55%

Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Fki¹,

Bouaziz²,

Chkir³

et al. 2013

Biologia plant.

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Date palm (Phoenix dactylifera L.) cv. Khenizi caulogenic meristems were initiated from achlorophyllous leaves excised from in vitro shoot cultures and then proliferated on a specific culture medium supplemented with 70 g dm-3 sucrose. Regeneration rates obtained when using standard vitrification, droplet-vitrification, and encapsulationvitrification protocols reached 26.7, 60.0, and 40.0 %, respectively. Only explants smaller than 3 mm in diameter were found to survive cryogenic treatments. Sucrose preculture, cold hardening and loading solution pretreatments showed significant effects on regeneration rates. Moreover, our results indicate that both sucrose preculture and cold acclimation of explants increased proline content. Cryopreservation of date palm tissue with high proliferation capacity can directly benefit large scale micropropagation projects. (Résumé d'auteur

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Section: ⎯⎯⎯⎯mentioning

confidence: 55%

Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Fki¹,

Bouaziz²,

Chkir³

et al. 2013

Biologia plant.

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“…However, Saker et al (2000) reported that RAPD and isozyme analyses could be successfully used to detect somaclonal variations in the date palm. In the same way, morphological abnormalities in somatic embryoderived date palm 'Sukkari' were described by Al-Mazroui et al (2007). Date palms 'Barhee' deriving from somatic embryos and natural offshoots were found to produce fruits of the same characteristics (Smith and Aynsley, 1995).…”

Section: Discussionmentioning

confidence: 91%

Multiple bud cultures of ‘Barhee’ date palm (Phoenix dactylifera) and physiological status of regenerated plants

Fki

Bouaziz

Kriaâ

et al. 2011

Journal of Plant Physiology

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“…High concentrations of micro-and macronutrients, RH and sugar content, plant growth regulators, low light intensity, low osmotic and water potentials in the medium, and limited carbon dioxide and oxygen gas exchange are all factors that positively impact the in vitro growing plants [22,23]. These variables achieve high proliferation rates, but they also frequently result in physiological, anatomical, and morphological disorders that impede ex vitro acclimatization and decrease the survival rate of plants [72,73]. High proliferation rates during the in vitro multiplication stage, successful ex vitro acclimatization of the plantlets, high plant survival rates, and some degree of automation are all necessary for effective commercial in vitro propagation [74].…”

Section: Discussionmentioning

confidence: 99%

Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets

2022

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One of the technological advancements in agricultural production is the tissue culture propagation technique, commonly used for mass multiplication and disease-free plants. The necessity for date palm tissue culture emerged from the inability of traditional propagation methods’ offshoots to meet the immediate demands for significant amounts of planting material for commercial cultivars. Tissue culture plantlets are produced in a protected aseptic in vitro environment where all growth variables are strictly controlled. The challenges occur when these plantlets are transferred to an ex vitro climate for acclimatization. Traditional glasshouses are frequently used; however, this has substantial mortality consequences. In the present study, a novel IoT-based automated ex vitro acclimatization system (E-VAS) was designed and evaluated for the acclimatization of date palm plantlets (cv. Khalas) to enhance their morpho-physiological attributes and reduce the mortality rate and the contamination risk through minimal human contact. The experimental findings showed that the morpho-physiological parameters of 6- and 12-month-old plants were higher when acclimatized in the prototype E-VAS compared to the traditional glasshouse acclimatization system (TGAS). The maximum plant mortality percentage occurred within the first month of the transfer from the in vitro to ex vitro environment in both systems, which gradually declined up to six months; after that, no significant plant mortality was observed. About 6% mortality was recorded in E-VAS, whereas 18% in TGAS within the first month of acclimatization. After six months of study, an overall 14% mortality was recorded in E-VAS compared to 41% in TGAS. The proposed automated system has a significant potential to address the growing demand for the rapid multiplication of tissue culture-produced planting materials since the plant survival rate and phenotype quality were much higher in E-VAS than in the conventional manual system that the present industry follows for commercial production.

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Morphological Abnormalities in Tissue Culture-Derived Date Palm (Phoenix Dactylifera L.)

Cited by 12 publications

References 0 publications

Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Multiple bud cultures of ‘Barhee’ date palm (Phoenix dactylifera) and physiological status of regenerated plants

Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets

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