Morphologic, cytogenetic and virologic studies in vitro of a malignant lymphoma from an African child

Abstract

A cell line of human lymphoblasts ( A L I ) , derived from a malignant lymphoma involving the jaw of an African child, has been established in a medium

Biochemical, Morphologic, and Gytogenetic Studies of Leukocytes Growing in Continuous Culture from Normal Individuals and from Patients with Cystinosis

“…Continuous suspension cultures of leukocytes may be derived from the peripheral blood of patients with lymphomas and leukemias and also from normal individuals [4,15,18,26,32]. The present report describes several of the properties of such cell lines cultured from normal individuals and those with cystinosis and illustrates the feasibility of utilizing these cells for investigations of the pathogenesis of cystinosis.…”

Resistance of Hamster Cells Transformed by Herpes Simplex Virus Type 2 to Superinfection by Herpes Simplex Viruses

“…This suggests that some cells in the population are sensitive to virus replication. This is analogous to the EB herpesvirus system where only a small percentage of transformed cells in a culture can synthesize virus at any one time [15][16][17]. The possibility, therefore, exists that there are three cell populations in cultures of 333-8-9 T No.…”

Preliminary observations on new lymphoblast strains (EB4, EB5) from Burkitt tumours in a British and a Ugandan patient.

“…On the basis of their behaviour and growth in vitro, their appearance in stained films and their fine structural organisation, the cells of the EB4 and EB5 strains are clearly of the same general lymphoblastic type as all the other cells established previously in culture from Burkitt tumours Pulvertaft, 1964;Stewart, Lovelace, Whang and Ngu, 1965;Epstein, Barr and Achong, 1965a;O'Conor and Rabson, 1965;Rabson, O'Conor, Baron, Whang, and Legallais, 1966;Osunkoya, 1966). In terms of detail, both the EB4 and EB5 cells are differentiated rather than undifferentiated lymphoblasts and therefore closer to EBI and EB3 cells (Epstein, Barr and Achong, 1965a) than to the undifferentiated EB2 (Epstein, Barr and Achong, 1965b), SL1 (Stewart, Lovelace, Whang and Ngu, 1965) or Raji cells (Pulvertaft, 1964;Epstein, Achong, Barr, Zajac, Henle and Henle, 1966); this is shown by their small size, regular rounded nuclei, relatively restricted cytoplasm and proliferation as single cells without forming regular clumps (Fig.…”