Resistance of Hamster Cells Transformed by Herpes Simplex Virus Type 2 to Superinfection by Herpes Simplex Viruses

Abstract

Cultures of hamster embryo fibroblasts originally trans formed in vitro by herpes simplex virus (HSV) type 2 previously irradiated with ultraviolet light were resistant to superinfection by HSV-1 and HSV-2. The degree of resistance was dependent upon the concentration of the superinfecting virus. At an MOI of 1.0 infectious virus particle per cell, significant virus replication occurred. At an MOI of 0.001 per cell, little or no virus replication was detected. In normal hamster embryo fibroblasts, virus replicated when either high or low MOI were used. Early events in the virus-replicative cycle were found to proceed normally. The results suggest that HSV can initiate and complete the infectious cycle in a limited number of herpes-transformed cells, a finding similar to that observed for Epstein-Barr herpesvirus systems.

Adenovirus-transformed cells restrict Herpes simplex virus replication

“…However, faulty attachment of HSV to transformed cells continues to remain suspect, since viral transformation of cells often results in profound alterations in the plasma membrane (17) which could conceivably affect viral attachment and penetration. However, if such changes occurred in HDC-17SA7 cells, it did not appear to affect virus adsorption since both HSV types attached rapidly to the plasma membrane, consistent with studies of HSV replication in cells transformed by other viruses (3, 6,7,18).…”

“…Nevertheless, in those studies which attempt to describe a resistance mechanism in virustransformed cells for HSV replication, none have found the limiting factor to be attachment of HSV to the plasma membrane (3,6,7,18). Indeed, studies in general of cells resistant to HSV infection, including nonviral transformed cells (2), have yet to yield a cell type which is capable of aborting the HSV replicative cycle at this level.…”

“…A cell line that normally supports the replication of herpes simplex virus types 1 and 2 became resistant to these viruses after transformation by simian adenovirus 7. Kinetic studies of the mechanism of resistance demonstrated that both herpesviruses were able to attach to the transformed cells and express some early genomic functions, as demonstrated by the presence of low levels of viral thymidine kinase.…”

Characteristics of Herpes Simplex Virus Resistance to Disodium Phosphonoacetate

“…Primary RK cells were prepared from weanling rabbit kidneys (21-28 days old) as previously described [13]. RK cells were used for routine preparation of HSV stocks as well as in a plaque assay of HSV [14]. The number of infectious particles in each HSV stock was assayed by the plaque method using Eagle's medium (BME) supple mented with 10% fetal bovine serum, 0.225% NaHCC>3 and 0.5% methylcellulose.…”

“…The evaluation of the replication of HSV-specific DNA was done using a previously published procedure [14], RK cell monolayers (8-oz. prescrip tion bottles) were grown for 24 h in BME supplemented with 10% dialyzed fetal bovine serum and 0.075% NaHCOs.…”