Morphologic and mechanical characteristics of engineered bovine arteries

Niklason, Laura E.; Abbott, William M.; Gao, Jinming; Klagges, Brian; Hirschi, Karen K.; Ulubayram, Kezban; Conroy, Nancy; Jones, Rosemary; Vasanawala, Ami; Sanzgiri, Seema; Langer, Robert

doi:10.1067/mva.2001.111747

Cited by 233 publications

(188 citation statements)

References 22 publications

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“…Vascular SMC were harvested by using standard explant techniques as described in ref. 45. Briefly, the saphenous vein was incised longitudinally, and the endothelial cells were removed by vigorously scraping the surface of the lumen with a scalpel blade to expose the medial layer of the vessel.…”

Section: Methodsmentioning

confidence: 99%

Relevance and safety of telomerase for human tissue engineering

Klinger

Blum

Hearn

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Tissue engineering holds the promise of replacing damaged or diseased tissues and organs. The use of autologous donor cells is often not feasible because of the limited replicative lifespan of cells, particularly those derived from elderly patients. Proliferative arrest can be overcome by the ectopic expression of telomerase via human telomerase reverse transcriptase (hTERT) gene transfection. To study the efficacy and safety of this potentially valuable technology, we used differentiated vascular smooth muscle cells (SMC) and vascular tissue engineering as a model system. Although we previously demonstrated that vessels engineered with telomerase-expressing SMC had improved mechanics over those grown with control cells, it is critical to assess the phenotypic impact of telomerase expression in donor cells, because telomerase upregulation is observed in >95% of human malignancies. To study the impact of telomerase in tissue engineering, expression of hTERT was retrovirally induced in SMC from eight elderly patients and one young donor. In hTERT SMC, significant lifespan extension beyond that of control was achieved without population doubling time acceleration. Karyotype changes were seen in both control and hTERT SMC but were not clonal nor representative of cancerous change. hTERT cells also failed to show evidence of neoplastic transformation in functional assays of tumorigenicity. In addition, the impact of donor age on cellular behavior, particularly the synthetic capability of SMC, was not affected by hTERT expression. Hence, this tissue engineering model system highlights the impact of donor age on cellular synthetic function that appears to be independent of lifespan extension by hTERT.tumorigenicity ͉ smooth muscle cells ͉ vascular graft

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Section: Methodsmentioning

confidence: 99%

Relevance and safety of telomerase for human tissue engineering

Klinger

Blum

Hearn

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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“…In this study, biodegradable polymer P(LLA-CL) NFS were seeded with porcine coronary artery smooth muscle cells (PCASMCs) and cultured in vitro for up to Sipehia et al (1996) and Deutsch et al (1999) collagen-based tissueengineered vascular grafts co-culture fibroblast, SMCs and ECs on collagen gels !500 mmHg the mechanical strength is below that required for artery (O2000 mmHg) Weinberg & Bell (1986) and Huynh et al (1999) Niklason et al (1999Niklason et al ( , 2001, Buttafoco et al (2006) and Heydarkhan-Hagvall et al (2006) 105 days, with the aim of evaluating the long-term viability of SMCs cultured on the scaffolds and to analyse the cellular effects on polymeric nanofibre degradation. We hypothesize that the degradation behaviour of polymeric polyester nanofibre will be milder than block materials, given the high surface area for diffusion of acidic degradation products.…”

Section: Introductionmentioning

confidence: 99%

Long-term viability of coronary artery smooth muscle cells on poly( l -lactide- co -ϵ-caprolactone) nanofibrous scaffold indicates its potential for blood vessel tissue engineering

Dong

Tan

Liao

et al. 2008

J. R. Soc. Interface.

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Biodegradable polymer nanofibres have been extensively studied as cell culture scaffolds in tissue engineering. However, long-term in vitro studies of cell-nanofibre interactions were rarely reported and successful organ regeneration using tissue engineering techniques may take months (e.g. blood vessel tissue engineering). Understanding the long-term interaction between cells and nanofibrous scaffolds (NFS) is crucial in material selection, design and processing of the tissue engineering scaffolds. In this study, poly(L-lactide-co-3-caprolactone) [P(LLA-CL)] (70 : 30) copolymer NFS were produced by electrospinning. Porcine coronary artery smooth muscle cells (PCASMCs) were seeded and cultured on the scaffold to evaluate cell-nanofibre interactions for up to 105 days. A favourable interaction between this scaffold and PCASMCs was demonstrated by cell viability assay, scanning electron microscopy, histological staining and extracellular matrix (ECM) secretion. Degradation behaviours of the scaffolds with or without PCASMC culture were determined by mechanical testing and gel permeation chromatography (GPC). The results showed that the PCASMCs attached and proliferated well on the P(LLA-CL) NFS. Large amount of ECM protein secretion was observed after 50 days of culture. Multilayers of aligned oriented PCASMCs were formed on the scaffold after two months of in vitro culture. In the degradation study, the PCASMCs were not shown to significantly increase the degradation rate of the scaffolds for up to 105 days of culture. The in vitro degradation time of the scaffold could be as long as eight months by extrapolating the results from GPC. These observations further supported the potential use of the P(LLA-CL) nanofibre in blood vessel tissue engineering.

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“…Les cellules remplissent les treillis en se multipliant et en produisant de la matrice extracellulaire. Des cellules endothéliales sont ensuite ensemencées à l'intérieur [6,7]. Ces prothèses comprennent seulement deux des trois tuniques vasculaires et présentent les désavantages associés à la présence de biomatériau: il est en effet difficile de dissocier les propriétés des tissus vivants reconstitués de celles du support inerte auquel ils sont intégrés, les biomatériaux pouvant alors interférer dans les mesures.…”

Section: Obtention De Vaisseaux Reconstitués Par Génie Tissulaireunclassified

“…Un des grands avantages de la reconstitution par auto-assemblage est d'obtenir, en l'absence de tout matériau synthétique, une résistance à l'éclatement suffisante pour permettre la greffe du vaisseau [8] et même le montage en bain d'organe pour étudier sa contraction [10]. D'autres vaisseaux obtenus à partir de cellules animales ont égale-ment pu être montés de la même façon, mais leur résistance méca-nique à l'étirement était renforcée par un matériau synthétique [6,7] ou hétérologue [11].…”

Section: Structure Et Fonction Des Vaisseaux Reconstituésunclassified

Vaisseaux humains reconstitués par génie tissulaire

Stoclet¹,

Laflamme

Auger

et al. 2004

Med Sci (Paris)

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La finalité du génie tissulaire est de reconstituer des tissus et des organes en laboratoire. Les progrès réali-sés dans ce domaine au cours des dernières décennies permettent aujourd'hui d'envisager la production d'organes complexes, tels que les vaisseaux sanguins, à partir de cellules humaines. Initialement conçus dans un but d'autogreffe [1], ces vaisseaux peuvent procurer d'intéressants modèles d'études humains.

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Morphologic and mechanical characteristics of engineered bovine arteries

Abstract: These findings aid in the systematic understanding of the effects of in vitro parameters on engineered vessels and will be useful for the translation of vessel culture techniques to human cells for the development of autologous human vascular grafts.

Cited by 233 publications

References 22 publications

Relevance and safety of telomerase for human tissue engineering

Relevance and safety of telomerase for human tissue engineering

Long-term viability of coronary artery smooth muscle cells on poly( l -lactide- co -ϵ-caprolactone) nanofibrous scaffold indicates its potential for blood vessel tissue engineering

Vaisseaux humains reconstitués par génie tissulaire

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