Morphogenic responses and biochemical alterations induced by the cover crop Urochloa ruziziensis and its component protodioscin in weed species

Menezes, Paulo Vinicius Moreira da Costa; Silva, Adriano Antonio; Mito, Márcio Shigueaki; Mantovanelli, Gislaine Cristiane; Stulp, Gabriel Felipe; Wagner, Ana Luiza; Constantin, Rodrigo Polimeni; Baldoqui, Débora Cristina; Silva, Raísa Gonçales; Carmo, Amanda Aparecida Oliveira do; Souza, Luiz Antônio de; Oliveira, Rubem Silvério de; Araniti, Fabrizio; Abenavoli, Maria Rosa; Ishii–Iwamoto, Emy Luiza

doi:10.1016/j.plaphy.2021.06.040

Cited by 3 publications

(5 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, plants treated with protodioscin in situ stained for H 2 O 2 confirmed the increased accumulation in roots. This result is in agreement with our previous work showing an increased content of ROS in I. grandifolia and D. insularis roots treated with protodioscin [51]. Furthermore, this increase was also recently observed in roots exposed to nerolidol [20] and benzoic acid [24] and was accompanied by root morphological alterations.…”

Section: Discussionsupporting

confidence: 93%

“…The inhibition of primary root elongation was even observed at low concentrations, with an ED 50 equal to 50 µM. A similar value was found in our previous study with the weed I. grandifolia (54 µg mL −1 ) and D. insularis (34 µg mL −1 ) [51]. An ED 50 of 240 µM was found in B. pilosa by Mito et al, (2019).…”

Section: Discussionsupporting

confidence: 88%

“…The in vitro assay revealed the inhibitory effects of protodioscin on Arabidopsis root growth, confirming the higher phytotoxic potential of this saponin [49][50][51], compared to other natural molecules [53]. The inhibition of primary root elongation was even observed at low concentrations, with an ED 50 equal to 50 µM.…”

Section: Discussionmentioning

confidence: 53%

“…A disturbance in mitochondrial respiratory activity and an oxidative stress condition was demonstrated in the primary roots of Bidens pilosa L. treated with protodioscin [50]. Recently, we demonstrated that the dicot Ipomoea grandifolia (Dammer) O'Donell and the monocot Digitaria insularis (L.) Fedde weed treated with protodioscin and butanolic extract of U. ruziziensis have drastic alterations in root morphology, including a reduction in the primary root length, the precocious appearance of lateral roots and reduction in root hairs [51]. The roots also exhibited features of advanced cell differentiation in the vascular cylinder.…”

Section: Introductionmentioning

confidence: 99%

“…The roots also exhibited features of advanced cell differentiation in the vascular cylinder. Although these morphogenic responses have suggested a disturbance in the auxin signalling, no direct evidence for this hypothesis has been examined [51].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The Steroid Saponin Protodioscin Modulates Arabidopsis thaliana Root Morphology Altering Auxin Homeostasis, Transport and Distribution

Wagner

Araniti

Bruno

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

To date, synthetic herbicides are the main tools used for weed control, with consequent damage to both the environment and human health. In this respect, searching for new natural molecules and understanding their mode of action could represent an alternative strategy or support to traditional management methods for sustainable agriculture. Protodioscin is a natural molecule belonging to the class of steroid saponins, mainly produced by monocotyledons. In the present paper, protodioscin’s phytotoxic potential was assessed to identify its target and the potential mode of action in the model plant Arabidopsis thaliana. The results highlighted that the root system was the main target of protodioscin, which caused a high inhibitory effect on the primary root length (ED50 50 μM) with morphological alteration, accompanied by a significant increase in the lateral root number and root hair density. Through a pharmacological and microscopic approach, it was underlined that this saponin modified both auxin distribution and transport, causing an auxin accumulation in the region of root maturation and an alteration of proteins responsible for the auxin efflux (PIN2). In conclusion, the saponin protodioscin can modulate the root system of A. thaliana by interfering with the auxin transport (PAT).

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Steroid Saponin Protodioscin Modulates Arabidopsis thaliana Root Morphology Altering Auxin Homeostasis, Transport and Distribution

Wagner

Araniti

Bruno

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

Secondary metabolites of Urochloa ruziziensis on growth, nodulation, and spectral responses of soybean

Cabral,

Araujo,

Bonifácio

et al. 2024

Plant Stress

View full text Add to dashboard Cite

Reduction of Weed Growth under the Influence of Extracts and Metabolites Isolated from Miconia spp.

et al. 2022

View full text Add to dashboard Cite

Weeds pose a problem, infesting areas and imposing competition and harvesting difficulties in agricultural systems. Studies that provide the use of alternative methods for weed control, in order to minimize negative impacts on the environment, have intensified. Native flora represents a source of unexplored metabolites with multiple applications, such as bioherbicides. Therefore, we aimed to carry out a preliminary phytochemical analysis of crude extracts and fractions of Miconia auricoma and M. ligustroides and to evaluate these and the isolated metabolites phytotoxicity on the growth of the target species. The growth bioassays were conducted with Petri dishes with lettuce, morning glory, and sourgrass seeds incubated in germination chambers. Phytochemical analysis revealed the presence of flavonoids, isolated myricetin, and a mixture of quercetin and myricetin. The results showed that seedling growth was affected in a dose-dependent manner, with the root most affected and the seedlings of the lettuce, morning glory, and sourgrass as the most sensitive species, respectively. Chloroform fractions and myricetin were the most inhibitory bioassays evaluated. The seedlings showed structural changes, such as yellowing, nonexpanded cotyledons, and less branched roots. These results indicate the phytotoxic potential of Miconia allelochemicals, since there was the appearance of abnormal seedlings and growth reduction.

show abstract

Morphogenic responses and biochemical alterations induced by the cover crop Urochloa ruziziensis and its component protodioscin in weed species

Cited by 3 publications

References 76 publications

The Steroid Saponin Protodioscin Modulates Arabidopsis thaliana Root Morphology Altering Auxin Homeostasis, Transport and Distribution

The Steroid Saponin Protodioscin Modulates Arabidopsis thaliana Root Morphology Altering Auxin Homeostasis, Transport and Distribution

Secondary metabolites of Urochloa ruziziensis on growth, nodulation, and spectral responses of soybean

Reduction of Weed Growth under the Influence of Extracts and Metabolites Isolated from Miconia spp.

Contact Info

Product

Resources

About