The Steroid Saponin Protodioscin Modulates Arabidopsis thaliana Root Morphology Altering Auxin Homeostasis, Transport and Distribution

Abstract

To date, synthetic herbicides are the main tools used for weed control, with consequent damage to both the environment and human health. In this respect, searching for new natural molecules and understanding their mode of action could represent an alternative strategy or support to traditional management methods for sustainable agriculture. Protodioscin is a natural molecule belonging to the class of steroid saponins, mainly produced by monocotyledons. In the present paper, protodioscin’s phytotoxic potential was assessed to identify its target and the potential mode of action in the model plant Arabidopsis thaliana. The results highlighted that the root system was the main target of protodioscin, which caused a high inhibitory effect on the primary root length (ED50 50 μM) with morphological alteration, accompanied by a significant increase in the lateral root number and root hair density. Through a pharmacological and microscopic approach, it was underlined that this saponin modified both auxin distribution and transport, causing an auxin accumulation in the region of root maturation and an alteration of proteins responsible for the auxin efflux (PIN2). In conclusion, the saponin protodioscin can modulate the root system of A. thaliana by interfering with the auxin transport (PAT).

Metabolomics Mechanism and Lignin Response to Laxogenin C, a Natural Regulator of Plants Growth

“…In general, low doses of exogenous auxin can promote plant growth. Meanwhile, high doses of exogenous auxins disturbed the homeostasis of plant hormones and caused plant death, for example, by altering auxin homeostasis, transport, and distribution to modulate root morphology and inhibit root growth [ 11 ]. However, most studies of spirostanes have focused on the relationship between regulatory effects and antioxidant enzymes instead of providing detailed insight into metabolic mechanisms.…”