Morphogenesis and regeneration from stomatal guard cell complexes of cotton ( Gossypium hirsutum L.)

Nobre, J.; Keith, D. J.; Dunwell, J. M.

doi:10.1007/s002990000281

Cited by 20 publications

(7 citation statements)

References 20 publications

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“…The differential induction of embryogenic calli by Coker 201, Coker 312 and YZ-1 explants reflected inherent genetic differences in accordance with the results recorded by Firoozabady and DeBoer (1993) and Nobre et al (2001). Genotype-dependence could be overcome in different degrees through optimizing culture factors as our previous report (Wu et al 2004), but it is still a key factor for somatic embryogenesis and plant regeneration in cotton.…”

Section: Discussionsupporting

confidence: 90%

Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton

Jin¹,

Zhang²,

Nie³

et al. 2006

Biologia plant.

124

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Hypocotyls of cotton (Gossypium hirsutum L.) cultivars cv. YZ-1, Coker 312 and Coker 201 were inoculated on Murashige and Skoog callus induction medium. YZ-1 exhibited a very high regeneration potential, with 81.9 % of the explants inoculated differentiated into embryogenic callus within 8 -10 weeks. During the process of callus maintenance (subculture for 1 to 3 years), the total embryos number in Coker 312 and Coker 201 calli dropped sharply, and the percentage of embryo germination decreased. On the contrary, the callus of YZ-1 consistently maintains a high frequency of plant regeneration after long-time subculture. Transgenic kanamycin-resistant calli of Coker 201 partially lost the ability of somatic embryogenesis and plant regeneration. The stress produced by the transformation procedure slightly affected somatic embryogenesis and plant regeneration of YZ-1, which showed minimum loss of plant regeneration ability.

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Section: Discussionsupporting

confidence: 90%

Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton

Jin¹,

Zhang²,

Nie³

et al. 2006

Biologia plant.

124

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“…In comparison with MSDK medium, MSDiP medium has low concentrations of 2,4-D (0.01 mg l -1 ) and kinetin was replaced with 0.5 mg l -1 2iP. 2iP has been proven to be effective for induction of SE in cotton (Trolinder and Goodin 1987;Nobre et al 2001). After 1 month, callus was spread on MSO medium (MS medium without any growth regulator), resulting in induction of globular embryos (Fig.…”

Section: Resultsmentioning

confidence: 99%

High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (Gossypium hirsutum L.) and efficient Agrobacterium-mediated transformation

Khan

Reddy

Leelavathi

2010

Plant Cell Tiss Organ Cult

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A highly efficient and reproducible regeneration system based on somatic embryogenesis in Gossypium hirsutum cv. Narasimha (NM), which has superior fiber qualities and is also used as a female parent in several hybrid cottons, has been developed. Embryogenic callus was obtained form both hypocotyls and cotyledonary leaves on Murashige and Skoog (MS) medium containing kinetin and 2,4-dichlorophenoxyacetic acid. Somatic embryogenesis was observed on hormone-free MS medium, but embryos did not grow well beyond globular stage. However, somatic embryos germinated well on MS medium containing B5 vitamins; addition of zeatin was found to be beneficial for their normal development. Most importantly, the media and culture conditions developed for NM were also found to be suitable for high-frequency somatic embryogenesis in Coker 310. In addition, the newly developed regeneration protocol has been successfully tested for genetic transformation through co-cultivation with Agrobacterium using embryogenic calli as explants. Molecular analysis confirmed the stable integration and expression of marker gene, green fluorescent protein (GFP). These results show that it is now possible to introduce foreign gene(s) directly into elite cultivar Narasimha with similar efficiency to in traditionally used Coker lines in a relatively short period of time. Development of efficient regeneration and transformation systems as demonstrated here should augment the introduction of new traits directly into cultivated varieties/ hybrids, reducing the time required for back-crossing and the costs for seed production, besides aiding genomic research in cotton.

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“…Callus induction frequency was more in hypocotyl explants than cotyledon explants isolated from 7 day-old seedlings. Significantly higher callus induction frequencies were observed for hypocotyl (97.3%) than cotyledon (94.6%) explants collected from 7 days-old seedlings irrespective of genotypes studied (Figures 2 (Sakhanokho et al, 1998;Nobre et al, 2001) and also in other species (Pereira et al, 2000;Dhar and Joshi, 2005). Such variations can be attributed to the physiological condition of the explant, which is determined by genetic factors (Nagarathna et al, 1991).…”

Section: Resultsmentioning

confidence: 99%

Factors influencing high callusing proliferation in cotton (Gossypium hirsutum L.)

Pushpa¹

2013

Afr. J. Plant Sci.

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In vitro regeneration of cotton (Gossypium spp.) has been a subject of intense research for the last two decades because of the commercial value of the crop. A study was conducted to assess the callusing potential of two local cotton genotype varieties viz., MCU-5 and SVPR-2 and two exotic genotypes, Coker 310 and 312. High significant difference was observed between media composition, genotype and explant types used for callus induction. Among the 24 media composition screened for callusing, the medium CIM3 (MS + 0.1 mg/l 2,4-D + 0.5 mg/l kinetin) exhibited successful and faster induction of calli. Callus initiation was found to be quicker from hypocotyls than cotyledons. Calli developed from hypocotyl explants were large, rough and friable, while cotyledon-derived calli were friable and medium sized. Significantly higher callus induction frequencies were observed in hypocotyl (97.3%) than cotyledon (95.8%) explants which were collected from 7 day-old seedlings irrespective of genotype. Explants collected from younger (4 day-old) and older (14 day-old) plants showed poor and low callus induction frequencies.

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Morphogenesis and regeneration from stomatal guard cell complexes of cotton ( Gossypium hirsutum L.)

Cited by 20 publications

References 20 publications

Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton

Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton

High-frequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (Gossypium hirsutum L.) and efficient Agrobacterium-mediated transformation

Factors influencing high callusing proliferation in cotton (Gossypium hirsutum L.)

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