Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton

Jin, Shuangxia; Zhang, X.; Nie, Yong; Guo, Xiaoping; Liang, Shuang; Zhu, Huayu

doi:10.1007/s10535-006-0082-5

Cited by 124 publications

(100 citation statements)

References 30 publications

(22 reference statements)

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“…The RNAi region containing a partially conserved domain and the specific 39 region of GbWRKY1 was cloned into the RNAi vector pHellsgate 4 through recombination reaction (Tan et al, 2013). The overexpression vector and RNAi vectors were introduced into G. hirsutum 'YZ1' plants by A. tumefaciens (strain EHA105)-mediated transformation as described previously (Jin et al, 2006). The overexpression vector was transferred into A. tumefaciens strain GV3101 to transform the Arabidopsis (Arabidopsis thaliana) ecotype Columbia-0 using the floral dip method .…”

Section: Gene Cloning Vector Construction and Plant Transformationmentioning

confidence: 99%

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

Luo

et al. 2014

Plant Physiology

133

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Plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. Previous research has demonstrated that DELLA proteins physically interact with JASMONATE ZIM-DOMAIN1 (JAZ1) and dynamically regulate the interaction of the gibberellin (GA) and jasmonate (JA) signaling pathways. However, whether and how the JAZ1-DELLA regulatory node is regulated at the transcriptional level in plants under normal growth conditions or during pathogen infection is not known. Here, we demonstrate multiple functions of cotton (Gossypium barbadense) GbWRKY1 in the plant defense response and during development. Although GbWRKY1 expression is induced rapidly by methyl jasmonate and infection by Verticillium dahliae, our results show that GbWRKY1 is a negative regulator of the JA-mediated defense response and plant resistance to the pathogens Botrytis cinerea and V. dahliae. Under normal growth conditions, GbWRKY1-overexpressing lines displayed GA-associated phenotypes, including organ elongation and early flowering, coupled with the down-regulation of the putative targets of DELLA. We show that the GA-related phenotypes of GbWRKY1-overexpressing plants depend on the constitutive expression of Gossypium hirsutum GhJAZ1. We also show that GhJAZ1 can be transactivated by GbWRKY1 through TGAC core sequences, and the adjacent sequences of this binding site are essential for binding specificity and affinity to GbWRKY1, as revealed by dual-luciferase reporter assays and electrophoretic mobility shift assays. In summary, our data suggest that GbWRKY1 is a critical regulator mediating the plant defense-to-development transition during V. dahliae infection by activating JAZ1 expression.

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Section: Gene Cloning Vector Construction and Plant Transformationmentioning

confidence: 99%

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

Luo

et al. 2014

Plant Physiology

133

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“…The conserved region of the four published F3H genes was selected as the RNAi target and cloned into the RNAi vector pHellsgate 4 through the Gateway system to produce pHG4-F3H (Helliwell et al, 2002). Transformation was performed via Agrobacterium tumefaciens (EHA105) according to a previously described method (Jin et al, 2006b). Because several regenerated transgenic seedlings failed in healthy root growth, a grafting method was performed to rescue these seedlings (Jin et al, 2006a).…”

Section: Plasmid Construction and Plant Transformationmentioning

confidence: 99%

A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

et al. 2013

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The cotton (Gossypium spp.) fiber is a unique elongated cell that is useful for investigating cell differentiation. Previous studies have demonstrated the importance of factors such as sugar metabolism, the cytoskeleton, and hormones, which are commonly known to be involved in plant cell development, while the secondary metabolites have been less regarded. By mining public data and comparing analyses of fiber from two cotton species (Gossypium hirsutum and Gossypium barbadense), we found that the flavonoid metabolism is active in early fiber cell development. Different flavonoids exhibited distinct effects on fiber development during ovule culture; among them, naringenin (NAR) could significantly retard fiber development. NAR is a substrate of flavanone 3-hydroxylase (F3H), and silencing the F3H gene significantly increased the NAR content of fiber cells. Fiber development was suppressed following F3H silencing, but the overexpression of F3H caused no obvious effects. Significant retardation of fiber growth was observed after the introduction of the F3H-RNA interference segment into the high-flavonoid brown fiber G. hirsutum T586 line by cross. A greater accumulation of NAR as well as much shorter fibers were also observed in the BC1 generation plants. These results suggest that NAR is negatively associated with fiber development and that the metabolism mediated by F3H is important in fiber development, thus highlighting that flavonoid metabolism represents a novel pathway with the potential for cotton fiber improvement.

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“…The overexpression vector, RNAi vectors, PGbPDF1::GUS, DBCD, DCD, DD, and PGbPDF1-1::GFP were introduced into cotton (YZ1) plants by A. tumefaciens-mediated (EHA105 and LBA4404) transformation as described by Jin et al (2006) and Li et al (2010). After regeneration, transformed cotton lines were checked by PCR (V-RNAi-F/R for RNAi constructs and V-gus-F/R for GUS-fused vectors) and Southern blotting.…”

Section: Plasmid Construction and Plant Transformationmentioning

confidence: 99%

GbPDF1 Is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2

et al. 2011

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Cotton (Gossypium spp.) fiber cells are seed trichomes derived from the epidermal layer of the cotton seed coat. The molecular components responsible for regulating fiber cell differentiation have not been fully elucidated. A cotton PROTODERMAL FACTOR1 gene (GbPDF1) was found to be expressed preferentially during fiber initiation and early elongation, with highest accumulation in fiber cells 5 d post anthesis. PDF1 silencing caused retardation of fiber initiation and produced shorter fibers and lower lint percentage compared with the wild type, indicating that the gene is required for cotton fiber development. Further analysis showed that a higher accumulation of hydrogen peroxide occurred in the RNA interference transgenic cotton lines. Meanwhile, the expression of several genes related to ethylene and pectin synthesis or sugar transport during cotton fiber growth was found to be significantly reduced in the PDF1-suppressed cotton. Three proteins interacting with GbPDF1 in yeast and in planta might involve cellular signaling or metabolism. GbPDF1 promoter::GUS constructs in transgenic cotton were predominantly expressed in the epidermis of ovules and developing fibers. Progressive deletions of the GbPDF1 promoter showed that a 236-bp promoter fragment was sufficient for basal GbPDF1 transcription in cotton. Mutation of putative regulatory sequences showed that HDZIP2ATATHB2, an element within the fragment, was essential for PGbPDF1-1 expression. The binding activity between this cis-element and nuclear extracts from fiber-bearing cotton ovules at 5 d post anthesis was specific. We conclude that GbPDF1 plays a critical role together with interaction partners in hydrogen peroxide homeostasis and steady biosynthesis of ethylene and pectin during fiber development via the core cis-element HDZIP2ATATHB2.

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Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton

Cited by 124 publications

References 30 publications

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

A Genetic and Metabolic Analysis Revealed that Cotton Fiber Cell Development Was Retarded by Flavonoid Naringenin

GbPDF1 Is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2

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