Moringa oleifera Protects SH-SY5YCells from DEHP-Induced Endoplasmic Reticulum Stress and Apoptosis

Amara, Ines; Ontario, Maria Laura; Scuto, Maria; Dico, Gianluigi Maria Lo; Sciuto, Sebastiano; Greco, Valentina; Abid‐Essefi, Salwa; Signorile, Anna; Salinaro, Angela Trovato; Calabrese, Vittorio

doi:10.3390/antiox10040532

Cited by 26 publications

(21 citation statements)

References 88 publications

(46 reference statements)

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“…24 A previous study showed that after 24 hours of exposure to DEHP, the caspase 3 activity increases in rat pheochromocytoma cells. 25 In our study, caspases 3 & 7 activity was observed (Figure 3) however it was not significantly different compared to the negative control. This low significance can be explained by the variability of the primary cultures that resulted from brain isolated by multiple adult zebrafish and not an established cell line.…”

Section: Discussioncontrasting

confidence: 44%

Mechanism involved in cell death induced by DEHP (Di-ethylhexyl phthalate) on primary brain cells from Zebrafish (Danio rerio)

Zayas¹,

Acevedo²,

Rivera³

et al. 2022

PPIJ

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Human and ecological exposure to chemical contaminants continue to increase given anthropogenic activities and sources. Phthalates are among the most persistent toxic chemicals found in aquatic systems. Phthalates belong to a family of chemical compounds that are heavily used in global manufacturing, distinguished for being able to provide durability and elasticity to plastic products, with poor water solubility and low evaporation. In terms of health impact, these compounds have been shown to breach the placental barrier having effect on human development and neurodegeneration. The main purpose of our study has been to assess the neurotoxicity of phthalates implementing the zebrafish (Danio rerio) model to determine the cell death induction of DEHP on brain cells. For many years, the zebrafish embryonic model has been used to study vertebrate development. Our study, in contrast, used adult zebrafish to resemble mature species that can be continuously exposed to low concentrations of DEHP. To assess the neurotoxicity, brain tissue from zebrafish was extracted via dissection, and primary neural cells dissociated to obtain primary cell subcultures. The neural cells where subsequently exposed to DEHP (Di-ethylhexyl phthalate) at concentrations from 10µM to 100µM for 48 hours to observe cell growth inhibition (GI50). The results indicate neural cell death at a GI50 of 62.9 µM. Apoptosis induction, caspase activation, mitochondrial membrane damage as well as autophagy cell death were analyzed to identify DEHP cell death mechanism at the GI50 concentration. In conclusion, this study clearly demonstrated that DEHP can induce apoptosis cell death on zebrafish neural brain cells in culture through the activation of an intrinsic apoptotic pathways with caspases 3 and 7 activation as well as autophagic pathway and mitochondrial damage. The presence of phthalates as well as other chemical contaminants on aquatic environments needs to be earnestly controlled in order to reduce neurotoxicity to aquatic species as well as human.

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Section: Discussioncontrasting

confidence: 44%

Mechanism involved in cell death induced by DEHP (Di-ethylhexyl phthalate) on primary brain cells from Zebrafish (Danio rerio)

Zayas¹,

Acevedo²,

Rivera³

et al. 2022

PPIJ

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“…All these results indicated that the MEHP exposure would activate the ER stress. There were some studies have also found MEHP would cause ER stress in other cells like SH‐SY5Y 40 . As an environmental endocrine disruptor, MEHP can cause homeostasis imbalance and increase ROS 41,42 .…”

Section: Discussionmentioning

confidence: 99%

“…There were some studies have also found MEHP would cause ER stress in other cells like SH-SY5Y. 40 As an environmental endocrine disruptor, MEHP can cause homeostasis imbalance and increase ROS. 41,42 Oxidative stress responses in the body may also induce the occurrence of ER stress.…”

Section: Discussionmentioning

confidence: 99%

Di(2‐ethylhexyl) phthalate induced thyroid toxicity via endoplasmic reticulum stress: In vivo and in vitro study

Zhao

et al. 2022

Environmental Toxicology

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Di(2-ethylhexyl) phthalate (DEHP) could induce thyroid injury but the mechanism was unclear. This study combined in vivo and in vitro experiments to clarify the mechanism. In vivo, the offspring of Sprague Dawley rats were gavaged with different doses of DEHP (5, 50, and 250 mg/[kgÁd]) from in utero to 12 weeks-old. Transcriptome sequencing was used to detect the mRNA expression profile of the offspring's thyroids. Differentially expressed genes were identified, followed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. In vitro, Nthyori 3-1 cells were exposed to DEHP's metabolite mono (2-ethylhexyl) phthalate (MEHP) to verify the pathway we found by KEGG analysis. The results indicated that DEHP could disorder the thyroid hormones. Compared with the offspring in control group, the mRNA levels of 656 genes were upregulated in the offspring exposed to 50 mg/(kgÁd) DEHP. The upregulated genes were enriched in the pathway of "protein processing in the endoplasmic reticulum (ER)." It indicated that the ER stress might play significant role in the thyroid toxicity induced by DEHP. In vitro, the mitochondrial membrane potential (ΔΨm) level of cells was decreased while the reactive oxygen species level was increased after MEHP exposure. MEHP increased the intracellular Ca 2+ level and induced ER stress. After ER stress was inhibited by the 4-phenylbutyric acid, the thyroid toxicity caused by MEHP was alleviated. Taken together, our results indicated that DEHP could induce thyroid toxicity by activating ER stress.

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“…Previous studies suggested that moringa exerts neuroprotective effects against DEHP (di‐[2‐Ethylhexyl] phthalate) a plasticizer linked to neurotoxicity‐induced cytotoxicity in SH‐SY5Y cells. Glucomoringin (GMG) from moringa transformed to isothiocyanate (ITC) on hydrolysis to form GMG‐ITC (Jaafaru et al, 2018) and protects cells by reducing ROS production, blockade of ER stress, and mitochondrial apoptosis (Amara et al, 2021).…”

Section: Molecular Mechanism Of M Oleifera In Neuroprotectionmentioning

confidence: 99%

“…Glucomoringin (GMG) from moringa transformed to isothiocyanate (ITC) on hydrolysis to form GMG-ITC (Jaafaru et al, 2018) and protects cells by reducing ROS production, blockade of ER stress, and mitochondrial apoptosis (Amara et al, 2021).…”

Section: Molecul Ar Mechanis M Of M Oleifera In Neuroprotec Ti Onmentioning

confidence: 99%

Neuroprotective potential of Moringa oleifera mediated by NF‐kB/Nrf2/HO‐1 signaling pathway: A review

Mundkar

Bijalwan

Soni

et al. 2022

Journal of Food Biochemistry

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Moringa oleifera is a traditional Indian herb belonging to the Moringaceae family, it is commonly known as the horse‐radish tree, drumstick, or sahajna. In developing countries, Moringa is used as feed for both humans and animals due to its well‐known antioxidant, anti‐inflammatory, and anti‐apoptotic properties owing to its several phytoconstituents including β‐carotene, quercetin, kaempferol, ascorbic acid, flavonoids, phenolic acid, rhamnose, glycosylates, glucomoringin, and isothiocyanates. These constituents help to maintain the brain antioxidant enzyme levels, mitochondrial functions, and neurogenesis, showing neuroprotective effects in several neurodegenerative disorders including Parkinson's Disease, Alzheimer's Disease, Huntington's Disease, and Amyotrophic lateral sclerosis. This review discusses various phytoconstituent of moringa and their therapeutic potential in various neurological disorders. Additionally, we also concise the safety and toxicity profile, of different molecular pathways involved in the neuroprotective effect of M. oleifera including M. oleifera nanoparticles for better therapeutic value. Practical applications Several clinical and preclinical studies on Moringa oleifera have been conducted, and the outcomes indicate moringa could be used in the treatment of brain disorders. As a result, we conclude that moringa and its nanoformulations could be employed to treat neurological problems. In the future, M. oleifera phytoconstituents could be evaluated against specific signaling pathways, which could aid researchers in discovering their mechanism of action. Furthermore, the use of moringa as a nutraceutical owing to its myriad pharmacological potential will go a long way in boosting the economy of countries that grow moringa on a large scale.

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Moringa oleifera Protects SH-SY5YCells from DEHP-Induced Endoplasmic Reticulum Stress and Apoptosis

Cited by 26 publications

References 88 publications

Mechanism involved in cell death induced by DEHP (Di-ethylhexyl phthalate) on primary brain cells from Zebrafish (Danio rerio)

Mechanism involved in cell death induced by DEHP (Di-ethylhexyl phthalate) on primary brain cells from Zebrafish (Danio rerio)

Di(2‐ethylhexyl) phthalate induced thyroid toxicity via endoplasmic reticulum stress: In vivo and in vitro study

Neuroprotective potential of Moringa oleifera mediated by NF‐kB/Nrf2/HO‐1 signaling pathway: A review

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