Neuroprotective potential of
            <i>Moringa oleifera</i>
            mediated by NF‐kB/Nrf2/HO‐1 signaling pathway: A review

Mundkar, Maroti; Bijalwan, Anjali; Soni, Divya; Kumar, Puneet

doi:10.1111/jfbc.14451

Cited by 20 publications

(19 citation statements)

References 121 publications

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“…MO attenuated ARV toxicity by upregulating the expression of NRF2 and its related antioxidants. This effect was previously demonstrated at the transcriptional and translational levels in various cell lines, including HepG2, HK-2, and V79-MZ cells, as well as animal models [ 97 , 98 , 99 ], C2C12 skeletal muscle cells [ 100 ] as well as in rat kidneys [ 101 , 102 ] and rat liver tissues [ 103 , 104 ]. MO contains several bioactive compounds, such as quercetin glucosinolates, isothiocyanate, flavonoids, and phenolic acids, that have been found to activate the NRF2-ARE [ 36 , 99 , 105 ].…”

Section: Discussionmentioning

confidence: 71%

Moringa oleifera Lam Leaf Extract Stimulates NRF2 and Attenuates ARV-Induced Toxicity in Human Liver Cells (HepG2)

Ndlovu

Chuturgoon

Ghazi

2023

Plants

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The World Health Organization (WHO) reported that there are 37 million individuals living with the human immunodeficiency virus (HIV) worldwide, with the majority in South Africa. This chronic disease is managed by the effective use of antiretroviral (ARV) drugs. However, with prolonged use, ARV drug-induced toxicity remains a clinically complex problem. This study investigated the toxicity of ARV drugs on mitochondria and the NRF2 antioxidant pathway and its possible amelioration using Moringa oleifera Lam (MO) leaf extracts. This medicinal plant has a range of functional bioactive compounds. Liver (HepG2) cells were treated with individual ARV drugs: Tenofovir disoproxil fumarate (TDF), Emtricitabine (FTC), and Lamivudine (3TC) for 96 h, followed by MO leaf extracts for 24 h. Intracellular ROS, cytotoxicity, lipid peroxidation, total and reduced glutathione (GSH), ATP, and mitochondrial polarisation were determined. Finally, protein (pNRF2, NRF2, SOD2, CAT, and Sirt3) and mRNA (NRF2, CAT, NQO1 SOD2, Sirt3, and PGC1α) expression were measured using Western blot and qPCR, respectively. TDF, FTC, and 3TC significantly increased intracellular ROS and extracellular levels of both MDA and LDH. ARVs also reduced the GSH and ATP levels and altered the mitochondrial polarization. Further, ARVs reduced the expression of NRF2 SOD2, Sirt3, CAT, NQO1, UCP2 and PGC1α mRNA and consequently pNRF2, NRF2, SOD2, Sirt3 and CAT protein. In contrast, there was a significant reduction in the extracellular MDA and LDH levels post-MO treatment. MO significantly reduced intracellular ROS while significantly increasing GSH, ATP, and mitochondrial membrane polarization. The addition of MO to ARV-treated cells significantly upregulated the expression of NRF2, SOD2, Sirt3, CAT, UCP2, PGC1α, and NQO1 mRNA and pNRF2, NRF2, SOD2, Sirt3 proteins. Thus, MO ameliorates ARV-induced hepatotoxicity by scavenging oxidants by inducing the NRF2 antioxidant pathway. MO shows great therapeutic potential and may be considered a potential supplement to ameliorate ARV drug toxicity.

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Section: Discussionmentioning

confidence: 71%

Moringa oleifera Lam Leaf Extract Stimulates NRF2 and Attenuates ARV-Induced Toxicity in Human Liver Cells (HepG2)

Ndlovu

Chuturgoon

Ghazi

2023

Plants

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“…Research has indicated that MO contains numerous bioactive compounds that have favorable impacts on human health. 18,19 The data obtained in this study after in silico docking analysis hence suggested that the bioactive compounds present in the MO leaf extract could potentially inhibit the over-activated PARP1 protein.…”

Section: Discussionmentioning

confidence: 73%

“…Research has indicated that MO contains numerous bioactive compounds that have favorable impacts on human health. 18, 19…”

Section: Discussionmentioning

confidence: 99%

Moringa oleifera Modulates MPTP-induced Mitochondrial Dysfunction in Parkinson’s Mouse Model: An in silico and in vivo Analysis

Singh,

Keshri,

Mishra

et al. 2023

Journal of Pharmacology and Pharmacotherapeutics

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Background: Parkinson’s disease (PD) is a movement-affecting neurodegenerative condition with an unclear etiology. Recent research suggests targeting poly-(adenosine 5-diphosphate-ribose) polymerase 1 (PARP1) as a potential therapeutic approach for PD treatment. Purpose: The purpose of this study is to assess the effect of an ethanolic extract of Moringa oleifera leaves (MOE) on a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinsonian mouse model, with a specific focus on investigating its potential to mitigate the effects of α-synuclein toxicity, oxidative stress–induced hyper-activation of PARP1, and mitochondrial dysfunction associated with PD pathology. Additionally, this study also intends to investigate the alterations in neurobehavioral and biochemical parameters associated with PD pathology. Methods: An in silico docking study was conducted to investigate the phytochemicals found in M. oleifera (MO, drumstick plant) leaves as the potent inhibitors of PARP1. An in vivo (neurobehavioral, biochemical, and western blot) study was conducted to assess the neuroprotective effect of MOE on the MPTP-induced Parkinsonian mouse model. Results: The results of in silico study showed that the phytochemicals found in MO leaves could be a potent inhibitor of PAPR1. The in vivo study results showed that MOE significantly ameliorated MPTP-induced neurobehavioral and biochemical deficits. MPTP-induced mitochondrial enzyme-complex deficits were found to be restored in MOE-treated mice. Additionally, the result obtained in the western blot analysis showed that MOE significantly restored the levels of tyrosine hydroxylase in MPTP-intoxicated mice. MOE enhanced the expression of the anti-apoptotic factor (Bcl-2) and suppressed the expression of pro-apoptotic factors (Bax and caspase-3). Additionally, the enhanced levels of α-synuclein and PARP1 were significantly suppressed by MOE. Conclusion: Our findings suggest that MOE may possess pharmacological properties that inhibit neuronal damage in MPTP-intoxicated mice. Thus, MOE could be used as a therapeutic agent that can protect dopaminergic neurons from PARP1-induced neuronal damage.

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“…Recent studies have indicated that many nutraceuticals, such as quercetin, avonoids, ascorbic acid, βcarotene, and phenolic acid, have neuroprotective effects and may alleviate the symptoms of PD [17,18]. This study investigated the effects of CA on the MPTP-induced zebra sh PD model.…”

Section: Discussionmentioning

confidence: 99%

Chicoric Acid from Health Foods Improves Motor Dysfunction in Zebrafish Parkinson’s Disease Model

Li²,

Zhang³

et al. 2023

Preprint

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Chicoric acid (CA) has been used as a nutritional supplement, health food, and medicine because of its antioxidant property. This study aimed to investigate whether CA can improve motor dysfunction in a zebrafish model of Parkinson’s disease (PD). AB-strain zebrafish larvae (24 hours post-fertilization) were incubated with 25 μM 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) with or without CA for 5 days. The levels of malondialdehyde, glutathione, and reactive oxygen species were measured, as were the activities of antioxidant enzymes in brain tissue. Additionally, the expression of molecules from the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway were assayed by western blot. Compared with the control group, zebrafish in the MPTP group had impaired motor function. Treatment with CA improved motor dysfunction and attenuated dopaminergic neuronal injury in the PD zebrafish model. CA treatment also reduced the levels of malondialdehyde and reactive oxygen species and increased the antioxidant enzymes and glutathione level. Furthermore, CA augmented the expression of Nrf2, heme oxygenase, quinone oxidoreductase 1, and glutamate-cysteine ligase in the brain of the PD zebrafish model. CA from health foods also improved motor dysfunction and attenuated dopaminergic neuronal injury in the MPTP-induced PD zebrafish model. Our findings suggest that the mechanism of action of CA is related to its inhibition of oxidative stress via regulation of the Nrf2 pathway.

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Neuroprotective potential of Moringa oleifera mediated by NF‐kB/Nrf2/HO‐1 signaling pathway: A review

Cited by 20 publications

References 121 publications

Moringa oleifera Lam Leaf Extract Stimulates NRF2 and Attenuates ARV-Induced Toxicity in Human Liver Cells (HepG2)

Moringa oleifera Lam Leaf Extract Stimulates NRF2 and Attenuates ARV-Induced Toxicity in Human Liver Cells (HepG2)

Moringa oleifera Modulates MPTP-induced Mitochondrial Dysfunction in Parkinson’s Mouse Model: An in silico and in vivo Analysis

Chicoric Acid from Health Foods Improves Motor Dysfunction in Zebrafish Parkinson’s Disease Model

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