Moringa oleifera Lam. leaf extract mitigates carbon tetrachloride-mediated hepatic inflammation and apoptosis via targeting oxidative stress and toll-like receptor 4/nuclear factor kappa B pathway in mice

Fathy, Samah M.; Mahmoud, Mohammed S.

doi:10.1016/j.fshw.2021.02.030

Cited by 15 publications

(10 citation statements)

References 36 publications

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“…After 4 weeks of treatment, the fasted mice were anesthetized using 10 mg/kg of xylazine or 110 mg/kg of ketamine and then euthanized via blood drainage from the heart [ 22 ]. The blood samples were centrifuged at 664 × g , and the serum was removed and kept at –70℃.…”

Section: Methodsmentioning

confidence: 99%

Artemisia annua L. Extracts Improved Insulin Resistance via Changing Adiponectin, Leptin and Resistin Production in HFD/STZ Diabetic Mice

et al. 2022

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Objectives: Insulin resistance (IR) is major cause of type 2 diabetes (T2D), and adipokines (e.g., adiponectin, leptin, and resistin) play an important role in insulin sensitivity. Medicinal plants are frequently used for T2D treatment. This study investigates the effect of Artemisia annua L. (AA) extracts on adipokines in mice with high-fat-diet (HFD)/streptozotocin (STZ)-induced T2D. Methods: We divided 60 mice into 12 groups (n = 5 per group): control, untreated T2D, treated T2D, and 9 other groups. T2D was induced in all groups, except controls, by 8 weeks of HFD and STZ injection. The treated T2D group was administered 250 mg/kg of metformin (MTF), while the nine other groups were treated with 100, 200, and 400 mg/kg of hot-water extract (HWE), cold-water extract (CWE), and alcoholic extract (ALE) of AA (daily oral gavage) along with 250 mg/kg of MTF for 4 weeks. The intraperitoneal glucose tolerance test (IPGTT) was performed, and the homeostasis model assessment of adiponectin (HOMA-AD) index and blood glucose and serum insulin, leptin, adiponectin, and resistin levels were measured. Results: Similar to MTF, all three types of AA extracts (HWEs, CWEs, and ALEs) significantly (p < 0.0001) decreased the area under the curve (AUC) of glucose during the IPGTT, the HOMA-AD index, blood glucose levels, and serum insulin, leptin, and resistin levels and increased serum adiponectin levels in the MTF group compared to the T2D group (p < 0.0001). The HWEs affected adipokine release, while the CWEs and ALEs decreased leptin and resistin production. Conclusion: Water and alcoholic AA extracts have an antihyperglycemic and antihyperinsulinemic effect on HFD/STZ diabetic mice. In addition, they decrease IR by reducing leptin and resistin production and increasing adiponectin secretion from adipocytes.

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Section: Methodsmentioning

confidence: 99%

Artemisia annua L. Extracts Improved Insulin Resistance via Changing Adiponectin, Leptin and Resistin Production in HFD/STZ Diabetic Mice

et al. 2022

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“…Our previous study recorded the following compounds in Moringa oleifera Lam. leaf ethanolic extract (MOLE); quercetagetin-7-O-glucoside, quercetin 3,5,7,3′,4′-pentamethyl ether, and βsitosterol along with other phytochemicals using gas chromatography-mass spectrometry (GC-MS) analysis (Fathy and Mahmoud 2021). Moreover, the total phenolic and the total avonoid contents in MOLE were also measured ( Fathy and Mahmoud 2021).…”

Section: Introductionmentioning

confidence: 99%

“…leaf ethanolic extract (MOLE); quercetagetin-7-O-glucoside, quercetin 3,5,7,3′,4′-pentamethyl ether, and βsitosterol along with other phytochemicals using gas chromatography-mass spectrometry (GC-MS) analysis (Fathy and Mahmoud 2021). Moreover, the total phenolic and the total avonoid contents in MOLE were also measured ( Fathy and Mahmoud 2021). Furthermore, MOL possesses pain relief, antidepression, antiin ammatory, immunomodulatory, and neuroprotective activities (Kou et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of Moringa oleifera Lam. leaf extract against oxidative stress, inflammation, depression, and apoptosis in a mouse model of hepatic encephalopathy

Mahmoud

El‐kott

AlGwaiz

et al. 2022

Environ Sci Pollut Res

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The present study aimed to assess the antioxidative, anti-in ammatory, antiapoptotic, and antidepression impacts of Moringa oleifera Lam. leaf ethanolic extract (MOLE) in the hippocampus and cerebral cortex of CCl 4 -induced mouse model of hepatic encephalopathy. High-performance liquid chromatography was used to detect marker compounds; rutin and β-sitosterol. Animals were divided into four groups; vehicle group, CCl 4 treated group, MOLE treated group, and (CCl 4 + MOLE) group treated with MOLE for 14 days before CCl 4 -induced neurotoxicity. MOLE decreased alanine aminotransferase, aspartate aminotransferase, corticosterone, and ammonia levels in serum and improved the antioxidant status of CCl 4 treated mice in the hippocampus and cerebral cortex. It reduced the expression of toll-like receptor (TLR)4, TLR2, myeloid differentiation primary response 88 (MYD88), and nuclear factor-kappa B (NF-κB) genes and the protein levels of the pro-inflammatory cytokines. MOLE also attenuated apoptosis, as revealed by the reduced expression of caspase3, and prevented histological deterioration. Furthermore, MOLE attenuated CCl 4 -induced anxiety and depression-like behavioral changes. Collectively, MOLE modulates neuroin ammation, oxidative stress, TLR4/2-MyD88/NF-κB signaling, and apoptosis in the hippocampus and cerebral cortex of the hepatic encephalopathy experimental model.

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“…[9,10,15] Toxicants that increase CCL4 gene expressions, such as methylmercury and carbon tetrachloride, have been reported to induce apoptosis in various cell systems. Carbon tetrachloride-induced apoptosis in mice liver cells, [81,82] and p53dependent apoptosis in other cell systems. [83] Methylmercury induced apoptosis in Hela cells, [84] rat pancreatic β cells, [85] and in rodents [86] ; the apoptosis induced in mice primary embryonal mouse fibroblasts was reported to be p53 dependent.…”

Section: Discussionmentioning

confidence: 99%

Diepoxybutane induces the p53‐dependent transactivation of the CCL4 gene that mediates apoptosis in exposed human lymphoblasts

Ewunkem

Deve

Harrison

et al. 2023

J Biochem & Molecular Tox

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Diepoxybutane (DEB) is the most toxic metabolite of the environmental chemical 1,3-butadiene. We previously demonstrated the occurrence of DEB-induced p53mediated apoptosis in human lymphoblasts. The p53 protein functions as a master transcriptional regulator in orchestrating the genomic response to a variety of stress signals. Transcriptomic analysis indicated that C-C chemokine ligand 4 (CCL4) gene expression was elevated in a p53-dependent manner in DEB-exposed p53-proficient TK6 cells, but not in DEB-exposed p53-deficient NH32 cells. Thus, the objective of this study was to determine whether the CCL4 gene is a transcriptional target of p53 and deduce its role in DEB-induced apoptosis in human lymphoblasts. Endogenous and exogenous wild-type p53 transactivated the activity of the CCL4 promoter in DEB-exposed lymphoblasts, but mutant p53 activity on this promoter was reduced by ∼80% under the same experimental conditions. Knockdown of the upregulated CCL4 mRNA levels in p53-proficient TK6 cells inhibited DEB-induced apoptosis by ∼45%-50%. Collectively, these observations demonstrate for the first time that the CCL4 gene is upregulated by wild-type p53 at the transcriptional level, and this upregulation mediates apoptosis in DEB-exposed human lymphoblasts.

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Moringa oleifera Lam. leaf extract mitigates carbon tetrachloride-mediated hepatic inflammation and apoptosis via targeting oxidative stress and toll-like receptor 4/nuclear factor kappa B pathway in mice

Cited by 15 publications

References 36 publications

Artemisia annua L. Extracts Improved Insulin Resistance via Changing Adiponectin, Leptin and Resistin Production in HFD/STZ Diabetic Mice

Artemisia annua L. Extracts Improved Insulin Resistance via Changing Adiponectin, Leptin and Resistin Production in HFD/STZ Diabetic Mice

Protective effect of Moringa oleifera Lam. leaf extract against oxidative stress, inflammation, depression, and apoptosis in a mouse model of hepatic encephalopathy

Diepoxybutane induces the p53‐dependent transactivation of the CCL4 gene that mediates apoptosis in exposed human lymphoblasts

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