Protective effect of Moringa oleifera Lam. leaf extract against oxidative stress, inflammation, depression, and apoptosis in a mouse model of hepatic encephalopathy

Mahmoud, Mohammed S.; El‐kott, Attalla F.; AlGwaiz, Hussah I.M.; Fathy, Samah M.

doi:10.1007/s11356-022-21453-x

Cited by 6 publications

(4 citation statements)

References 54 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results align with previous research; significant antiepileptic and anxiolytic effects were seen with Aqueous Extract M. oleifera (250, 375, and 500 mg/kg, i. p.) [8]. Mahmoud, M. S, et al, also reported M. oleifera leaf extract attenuated CCl4-induced anxiety and behavioral changes such as depression [23].…”

Section: Effect Of Mow On Behavior Abnormalitiessupporting

confidence: 92%

Neuroprotective and Antioxidant Activities of Aqueous Extract Moringa Oleifera Leaves

et al. 2022

View full text Add to dashboard Cite

Objective: To investigate the neuroprotective and antioxidant effects of leaves aqueous extract Moringa oleifera (MW) in chronic stress mouse models. Methods: Water immersion and stress restraint for 16 d to obtain a chronic stress model animal. Moringa extract flour dissolved in Aquades, dose 800 mg/kg for 23 d, for chronic Stress+MOW group. Fluoxetine in aquades at a dose of 18 mg/kg BW for 23 d for chronic stress group+Fluoxetine. Aquades were given to normal mice (group N), and mice under chronic stress conditions (chronic stress control group). Furthermore, measure behavioral abnormalities by testing depressive behavior and oxidative stress parameters such as anxiety, Brain-derived neurotrophic factors (BDNF). Results: Moringa oleifera water extract administration can improve behavioral disorders caused by stress by decreasing immobility time on the Force swim test, increasing time in the middle area, and increasing the number of returns to center areas on the Open field test. When chronically stressed mice were given fluoxetine and MOW, their MDA levels (p=0.008 and 0.041, respectively) and SOD activity (p=0.001 and 0.004) decreased significantly compared to the chronic stress control group. In contrast, Catalase activity increased significantly in chronically stressed mice given fluoxetine and MOW compared to the chronic stress control group (p=0.010 and 0.013). Administration of fluoxetine and MOW may increase the expression of mRNA BDNF compared to the chronic stress control group (p=0.000 and 0.013). Conclusion: The study found that MOW can improve behavioral abnormalities, namely anxiety and depression behavior caused by chronic stress exposure, through antioxidant pathways and oxidant systems, and also BDNF

show abstract

Section: Effect Of Mow On Behavior Abnormalitiessupporting

confidence: 92%

Neuroprotective and Antioxidant Activities of Aqueous Extract Moringa Oleifera Leaves

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Consequently, this dual effect translated into a reduction in myocardial infarct size and cell apoptosis, underscoring the role of BS as a cardioprotective agent [36]. These findings suggest that BS exerts anti-apoptotic effects in inflammatory states [37].…”

Section: Discussionmentioning

confidence: 82%

β-Sitosterol Enhances Lung Epithelial Cell Permeability by Suppressing the NF-κB Signaling Pathway

Chen,

Ren

et al. 2023

Discovery Medicine

View full text Add to dashboard Cite

Background: The dysregulation between pro-inflammatory and anti-inflammatory responses during sepsis is a crucial factor in driving sepsis progression. Acute lung injury (ALI) resulting from excessive production and accumulation of inflammatory mediators in the lungs contributes to impaired lung barrier function. The activation of the NF-κB signaling pathway during inflammation leads to the transcriptional activation of multiple inflammatory genes. Given the plausible impact of NF-κB signaling suppression in mitigating lung injury, substantive evidence demonstrates beta-sitosterol (BS)'s proficient ability to block NF-κB activation. Therefore, the aim of the present investigation was to delve into the impacts of BS in the context of sepsis-induced acute lung injury, employing both a mouse model and a model involving lung epithelial cells. Methods: Sepsis-induced lung injury was simulated in mice through cecum ligation and puncture (CLP). To emulate injury in murine lung epithelial (MLE-12) cells, an experiment involving lipopolysaccharide (LPS) was administered. Evaluation of alterations in lung tissue permeability encompassed techniques such as lung wet/dry (W/D) mass ratio, Evans blue staining, and quantification of total protein concentration in bronchoalveolar lavage fluid (BALF). Lung tissue histopathological shifts were ascertained via hematoxylin and eosin (HE) staining. Additionally, the concentrations of inflammatory cytokines IL-6 and TNF-α were quantified in every lung tissue and cell group by implementing enzyme-linked immunosorbent assay (ELISA). Protein quantification for signal biomarkers was carried out using Western blotting and immunofluorescence methodologies. In tandem, the assessment of MLE-12 cell permeability was conducted by evaluating fluorescein isothiocyanate (FITC)-dextran extravasation. Results: BS mitigated lung tissue pathologies, reduced inflammatory factors, and lowered tissue and cell permeability. BS inhibited NF-κB signaling and increased claudin-4 and claudin-5 expression, enhancing septic lung epithelial cell permeability. Conclusions: Through suppressing the NF-κB signaling cascade, BS effectively curtails the levels of inflammatory mediators. Simultaneously, it orchestrates the modulation of claudin-4 and claudin-5 expression, culminating in the augmentation of lung epithelial cell barrier competence, thus improving sepsis-induced lung injury.

show abstract

“…One study showed that in a model of myocardial ischemia-reperfusion injury, BS upregulated the expression of Bcl-2 and downregulated the expression of caspase-3, which reduced myocardial infarct size and cells apoptosis, playing a role of cardioprotective [33]. Thus, BS exert anti-apoptotic effects in in ammatory states [34].…”

Section: Discussionmentioning

confidence: 99%

β-sitosterol improves the permeability of lung epithelial cells by inhibiting the NF-kB pathway

Chen

Ren

et al. 2023

Preprint

View full text Add to dashboard Cite

Objective: To investigate the mechanism of the protective effect of beta-sitosterol (BS) on sepsis-induced lung injury. METHODS: A mouse model of sepsis-induced lung injury was established by cecum ligation and puncture (CLP). The murine lung epithelial (MLE-12) cells injury model was established by challenging LPS. Evans blue staining, lung wet/dry mass ratio (W/D), and the total protein concentration in bronchoalveolar lavage fluid (BALF) were used to assess the changes in lung tissue permeability. HE staining was performed to observe the histopathological changes in lung tissues. ELISA was examined to determine IL-6 and TNF-α inflammatory factors in each group of lung tissues and cells. The protein levels of claudin-4, claudin-5, Bcl-2, Bax, p65 and p-p65 were detected by western blotting or immunofluorescence. Fluorescein isothiocyanate (FITC)-dextran extravasation as a measure of MLE-12 cells permeability. RESULTS: BS attenuated lung tissue pathological changes caused by sepsis, reduced the level of inflammatory factors and decreased the permeability of lung tissue and MLE-12 cells. Moreover, BS inhibited NF-kB signaling pathway to upregulate tight junction proteins claudin-4 and claudin-5 thereby improving septic lung epithelial cell permeability. CONCLUSION: BS reduces the level of inflammatory factors by inhibiting NF-kB signaling pathway and regulates the expression of tight junction proteins claudin-4 and claudin-5 to improve the barrier function of lung epithelial cells, thus improving lung injury caused by sepsis.

show abstract

Protective effect of Moringa oleifera Lam. leaf extract against oxidative stress, inflammation, depression, and apoptosis in a mouse model of hepatic encephalopathy

Cited by 6 publications

References 54 publications

Neuroprotective and Antioxidant Activities of Aqueous Extract Moringa Oleifera Leaves

Neuroprotective and Antioxidant Activities of Aqueous Extract Moringa Oleifera Leaves

β-Sitosterol Enhances Lung Epithelial Cell Permeability by Suppressing the NF-κB Signaling Pathway

β-sitosterol improves the permeability of lung epithelial cells by inhibiting the NF-kB pathway

Contact Info

Product

Resources

About