Morin applied in speciation of aluminium in natural waters and biological samples by reversed-phase high-performance liquid chromatography with fluorescence detection

Lian, Hong-zhen; Kang, Yufen; Bi, Shuping; Arkin, Yasin; Shao, Dalin; Chen, Yijun; Dai, Lemei; Tian, Lianhua

doi:10.1007/s00216-003-1936-8

Cited by 34 publications

(17 citation statements)

References 37 publications

(39 reference statements)

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“…It was reported that morin detects cell wall-bound Al (Ahn et al , 2002), but Eticha et al (2005) unequivocally showed that morin could not stain cell wall-bound Al. The results of Eticha et al support the conclusion that morin is not able to stain Al in high stability complexes (Lian et al , 2003). …”

Section: Introductionmentioning

confidence: 76%

“…Browne et al (1990 a , b ) stated that morin is a reagent which reliably complexes Al, with ‘minimized disturbance’. In that study, the fluorescence of the Al–morin complex was directly related to Al 3+ and Al hydroxy complexes, indicating that morin forms complexes only with inorganic monomeric Al species but not with Al–organic acid complexes (Lian et al , 2003). The precise Al–morin complex formation and underlying stability constants remained unclear for a long time.…”

Section: Introductionmentioning

confidence: 83%

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Aluminium localization in root tips of the aluminium-accumulating plant species buckwheat (Fagopyrum esculentum Moench)

Klug¹,

Specht²,

Horst³

2011

Journal of Experimental Botany

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Aluminium (Al) uptake and transport in the root tip of buckwheat is not yet completely understood. For localization of Al in root tips, fluorescent dyes and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) were compared. The staining of Al with morin is an appropriate means to study qualitatively the radial distribution along the root tip axis of Al which is complexed by oxalate and citrate in buckwheat roots. The results compare well with the distribution of total Al determined by LA-ICP-MS which could be reliably calibrated to compare with Al contents by conventional total Al determination using graphite furnace atomic absorption spectrometry. The Al localization in root cross-sections along the root tip showed that in buckwheat Al is highly mobile in the radial direction. The root apex predominantly accumulated Al in the cortex. The subapical root section showed a homogenous Al distribution across the whole section. In the following root section Al was located particularly in the pericycle and the xylem parenchyma cells. With further increasing distance from the root apex Al could be detected only in individual xylem vessels. The results support the view that the 10 mm apical root tip is the main site of Al uptake into the symplast of the cortex, while the subapical 10–20 mm zone is the main site of xylem loading through the pericycle and xylem parenchyma cells. Progress in the better molecular understanding of Al transport in buckwheat will depend on the consideration of the tissue specificity of Al transport and complexation.

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Section: Introductionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 83%

Aluminium localization in root tips of the aluminium-accumulating plant species buckwheat (Fagopyrum esculentum Moench)

Klug¹,

Specht²,

Horst³

2011

Journal of Experimental Botany

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“…However, the 8-hydroxyquinoline method (and its variants), as well as some other chromogenic and fluorometric analytical techniques, eg. ferron (Jallah & Smyth 1998), pyrocatechol violet (Flaten & Lund 1997;Hils et al 1999), chrome-azurol S (Simpson et al 1997) or morin (Lian et al 2003), can only be useful in kinetic reaction studies to operationally define Al fractions, but actual speciation within these fractions is frequently uncertain. Even when such separation of Al species was achieved (Tsunoda et al 2001), the sensitivity of ICP-MS as a quantification technique was better than that of the 8-hydroxyquinoline-5-sulphonic acid method.…”

Section: Quantification Techniquesmentioning

confidence: 98%

Aluminium cycling in the soil-plant-animal-human continuum

Rengel

2004

Biometals

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A critical review of the literature on Al toxicity in plants, animals and humans reveals a similar mode of Al action in all living organisms, namely interference with the secondary messenger system (phosphoinositide and cytosolic Ca2+ signalling pathways) and enhanced production of reactive oxygen species resulting in oxidative stress. Aluminium uptake by plants is relatively quick (across the intact plasma membrane in < 30 min and across the tonoplast in < 1 h), despite huge proportion of Al being bound in the cell wall. Aluminium absorption in the animal/human digestive system is low (only about 0.1% of daily Al intake stays in the human body), except when Al is complexed with organic ligands (eg. citrate, tartarate, glutamate). Aluminium accumulates in bones and brain, with Al-citrate and Al-transferrin complexes crossing the blood-brain barrier and accumulating in brain cells. Tea plant and other Al-accumulator plant species contain large amounts of Al in the form of non-toxic organic complexes.

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“…Nevertheless, for analytical purpose, the advent of atomic absorption spectroscopy (AAS) and inductively coupled plasma atomic emission spectrometry (ICP-AES) turned out to be an improvement for aluminium determination, compared to the traditional fluorometric method. Fluorescence of aluminium complexes is now rather used in highly selective and sensitive methods such as high-performance liquid chromatography with post-column fluorescence detection [13], sequential injection method with fluorescence sensing [14], visual fluorometry on thin layers [15], etc.…”

Section: Introductionmentioning

confidence: 99%