Monovalent cation metabolism and cytopathic effects of poliovirus-infected HeLa cells

Nair, C. N.

doi:10.1128/jvi.37.1.268-273.1981

Cited by 34 publications

(11 citation statements)

References 20 publications

(23 reference statements)

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“…thesis) showed that the viruses do cross-react; anti-poliovirus 1 sera reduced the cytopathogenic effect of porcine enterovirus 1,000-fold in a cell culture assay. The adsorption of poliovirus to cells has been reported to cause an influx of Ca2+ into the cells (26). The increase in the intracellular Ca2+ concentration induced by poliovirus adsorption results in the depolymerization of cellular microfilaments (16).…”

Section: Discussionmentioning

confidence: 99%

Effect of enteroviruses on adherence to and invasion of HEp-2 cells by Campylobacter isolates

Konkel

Joens

1990

Infect Immun

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Coinfection of HEp-2 epithelial cells with coxsackievirus B3, echovirus 7, poliovirus (LSc type 1), porcine enterovirus, and Campylobacter isolates was performed to determine if a synergistic effect could be obtained. The invasiveness of Campylobacterjejuni ATCC 33560 was significantly increased for HEp-2 cells preinfected with echovirus 7, coxsackievirus B3, and UV-inactivated (noninfectious) coxsackievirus B3 particles. Additionally, the invasiveness of C. jejuni M96, a clinical isolate, was significantly increased for HEp-2 cells preinfected with coxsackievirus B3. Poliovirus and porcine enterovirus had no effect on C. jejuni ATCC 33560 adherence and invasiveness. Furthermore, poliovirus had no effect on the ability of C. jejuni M96 to adhere to and invade HEp-2 cells. Campylobacter hyointestinalis and Campylobacter mucosalis, two noninvasive isolates, did not invade virus-infected HEp-2 cells. The increase in the invasiveness of C. jejuni appeared to be the result of specific interactions between the virus and the HEp-2 cell membrane. The data suggest that the invasiveness of Campylobacter spp. is dependent upon the inherent properties of the organism. Virus-induced cell alterations can potentiate the invasiveness of virulent Campylobacter spp. but are not sufficient to allow internalization of noninvasive bacteria.

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Section: Discussionmentioning

confidence: 99%

Effect of enteroviruses on adherence to and invasion of HEp-2 cells by Campylobacter isolates

Konkel

Joens

1990

Infect Immun

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“…CVB3 protein 2B has been shown to modify endoplasmic re-ticulum and plasma membrane permeability (14), causing an increase in the cytosolic free calcium concentration (28,67) and membrane lesions which may facilitate viral progeny release. Ionic gradients collapse (40,52), and the phospholipase activity is altered (24,29). CVB3 infection of HeLa cells results in tyrosine phosphorylation of two cellular proteins at 4 h postinfection, and inhibition of these phosphorylations significantly reduces viral progeny production (27).…”

mentioning

confidence: 99%

Caspase Activation and Specific Cleavage of Substrates after Coxsackievirus B3-Induced Cytopathic Effect in HeLa Cells

Carthy

Granville

Watson

et al. 1998

J Virol

161

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Coxsackievirus B3 (CVB3), an enterovirus in the familyPicornaviridae, induces cytopathic changes in cell culture systems and directly injures multiple susceptible organs and tissues in vivo, including the myocardium, early after infection. Biochemical analysis of the cell death pathway in CVB3-infected HeLa cells demonstrated that the 32-kDa proform of caspase 3 is cleaved subsequent to the degenerative morphological changes seen in infected HeLa cells. Caspase activation assays confirm that the cleaved caspase 3 is proteolytically active. The caspase 3 substrates poly(ADP-ribose) polymerase, a DNA repair enzyme, and DNA fragmentation factor, a cytoplasmic inhibitor of an endonuclease responsible for DNA fragmentation, were degraded at 9 h following infection, yielding their characteristic cleavage fragments. Inhibition of caspase activation by benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (ZVAD.fmk) did not inhibit the virus-induced cytopathic effect, while inhibition of caspase activation by ZVAD.fmk in control apoptotic cells induced by treatment with the porphyrin photosensitizer benzoporphyrin derivative monoacid ring A and visible light inhibited the apoptotic phenotype. Caspase activation and cleavage of substrates may not be responsible for the characteristic cytopathic effect produced by picornavirus infection yet may be related to late-stage alterations of cellular homeostatic processes and structural integrity.

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“…During poliovirus infection there are drastic modifications in the concentrations of intracellular monovalent cations, such that sodium ions enter into cells whereas potassium ions leak out (15,17). Although changes in monovalent cation concentrations and pH during poliovirus infection of HeLa cells have been extensively analyzed (3,4), virtually nothing is known about the potential modifications in the concentration of calcium ions.…”

mentioning

confidence: 99%

“…that reaches a 10-fold increase by the fourth hour p.i. The time when the concentration of calcium ions is enhanced in the cytoplasm of poliovirus-infected cells is coincident with the increase in membrane permeability to monovalent cations (15,17). The kinetics of poliovirus protein synthesis in this experiment was analyzed in parallel each hour after infection.…”

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confidence: 99%

Enhanced intracellular calcium concentration during poliovirus infection

Irurzun

Arroyo

Álvarez

et al. 1995

J Virol

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The infection of human fibroblasts by poliovirus leads to a notable increase in the intracellular calcium concentration, [Ca2؉ ] i , measured by microfluorimetry or by flow cytometry. [Ca 2؉ ] i increases from 2 to 3 h postinfection, and by the fifth hour there is a 5-to 10-fold increase in [Ca 2؉ ] i . At this time postinfection there is active viral protein synthesis. The modifications in [Ca 2؉ ] i are not observed in the presence of cycloheximide, guanidine, or Ro 09-0179, indicating that virus gene expression is required for the increase in [Ca 2؉ ] i . Attempts to identify the source of the intracellular Ca 2؉ by using different inhibitors of calcium fluxes suggest that calcium enters from the culture medium through voltage-sensitive calcium channels.

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Monovalent cation metabolism and cytopathic effects of poliovirus-infected HeLa cells

Cited by 34 publications

References 20 publications

Effect of enteroviruses on adherence to and invasion of HEp-2 cells by Campylobacter isolates

Effect of enteroviruses on adherence to and invasion of HEp-2 cells by Campylobacter isolates

Caspase Activation and Specific Cleavage of Substrates after Coxsackievirus B3-Induced Cytopathic Effect in HeLa Cells

Enhanced intracellular calcium concentration during poliovirus infection

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