Effect of enteroviruses on adherence to and invasion of HEp-2 cells by Campylobacter isolates

Konkel, Michael E.; Joens, Lynn A.

doi:10.1128/iai.58.4.1101-1105.1990

Cited by 17 publications

(9 citation statements)

References 31 publications

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“…16−18 There is documentation of concurrent infections precipitating C. jejuni enteritis. 19 These observations support the notion that this organism is capable of producing a spectrum of disease scenarios depending on the immune status of the host, virulence determinants of the bacterium, and complexity of concurrent infections.…”

Section: Introductionsupporting

confidence: 65%

Enhancement of Disease and Pathology by Synergy of Trichuris Suis and Campylobacter Jejuni in the Colon of Immunologically Naive Swine

Mansfield¹,

Gauthier²,

Abner³

et al. 2003

The American Journal of Tropical Medicine and Hygiene

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Campylobacter jejuni, a leading cause of bacterial gastroenteritis, has different age distribution and disease expression in developing and developed countries, which may be due to the endemnicity of infection and the age of acquisition of immunity. Differences in disease expression are not solely dependent on the C. jejuni strain or virulence attributes. Another modulating factor in developing countries may be endemic nematode infections such as Trichuris, which drive type 2 cytokine responses and down-regulate type 1 immune responses. In this study, three-day-old germfree pigs given dual infections with Trichuris suis and C. jejuni had more frequent, more severe diarrhea and severe pathology than pigs given no pathogens, only T. suis, or only C. jejuni. These pigs had significant hemorrhage and inflammatory cell infiltrates in the proximal colon where adult worms were found, and abscessed lymphoglandular complexes in the distal colon with intracellular C. jejuni. Pigs given only C. jejuni had mild clinical signs and pathology, and bacteria in feces or extracellular sites. Pigs given T. suis or no pathogens had no disease and minimal pathology. Thus, these agents synergized to produce significant disease and pathology, which was site specific.

show abstract

Section: Introductionsupporting

confidence: 65%

Enhancement of Disease and Pathology by Synergy of Trichuris Suis and Campylobacter Jejuni in the Colon of Immunologically Naive Swine

Mansfield¹,

Gauthier²,

Abner³

et al. 2003

The American Journal of Tropical Medicine and Hygiene

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“…The panel contains nine antimicrobials: azithromycin, ciprofloxacin, clindamycin, erythromycin, florfenicol, gentamicin, nalidixic acid, telithromycin, and tetracycline. Panels were incubated under microaerobic conditions at 42 ± 1°C for 24 h, and Campylobacter jejuni isolate 33560 (Konkel and Joens, 1990) was used as a quality control organism.…”

Section: Campylobacter Sppmentioning

confidence: 99%

Antibiotic Resistance Patterns of Major Zoonotic Pathogens from All-Natural, Antibiotic-Free, Pasture-Raised Broiler Flocks in the Southeastern United States

et al. 2016

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The use of antibiotics in agroecosystems has been implicated in the rise in antibiotic resistance (AR), which can affect environmental, animal, and human health. To determine the environmental impact of antibiotic use in agroecosystems, appropriate background levels of AR in agricultural environments in the absence of antibiotic application must be determined. Therefore, to determine background levels of AR in broiler production, four target microbes (, , , and ) were isolated from 15 all-natural, antibiotic-free, pasture-raised broiler flocks from six farms within the southeastern United States. The AR profiles of these isolates were characterized using the CDC National Antimicrobial Resistance Monitoring System for Enteric Bacteria (NARMS), and these resistance patterns were compared across target microbes and farms and throughout the life cycle of the flocks along the farm-to-fork continuum. Antibiotic resistances were most prevalent in and and least prevalent in . Although and were isolated from the same farms and characterized using the same NARMS plates, they exhibited distinct AR profiles, with demonstrating clear farm-specific resistance patterns. Multidrug resistance rates (three or more antibiotics), in order of prevalence, were (63.9%), (36.0%), (12.7%), and (1.4%). The results of this study demonstrate the variability in background AR among major food safety-related microbes, even when isolated from similar production and processing samples from the same farms, and indicate the need for the proper design of future broiler production studies to account for this highly dynamic background AR.

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“…Konkel and Joens also found that Campylobacter species were invasive in HEp-2 cells (13). Nonetheless, they demonstrated higher levels of HEp-2 cell invasion when C. jejuni was coinfected with enteroviruses (14).…”

mentioning

confidence: 92%

Campylobacter jejuni motility and invasion of Caco-2 cells

et al. 1995

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We investigated the influence of motility on Campylobacter jejuni binding and invasion of Caco-2 cells. C. jejuni was motile in soft agar at basic (pH 8.5) and neutral pH values representative of the intestinal environment. However, C. jejuni was immobilized at pH 5.0. The inability of C. jejuni to swarm on soft agar at pH 5.0 was not related to flagellar depolymerization or loss of viability. In tissue culture medium, C. jejuni displayed typical periods of straight swimming punctuated by tumbling behavior. This behavior was altered when the viscosity of the medium was adjusted to mimic the viscosity of intestinal mucus. C. jejuni showed longer periods of straight swimming with significantly increased velocity followed by pauses instead of tumbles. The binding and invasion of C. jejuni in Caco-2 cells also increased significantly in high-viscosity growth medium. We speculate that the swimming behavior of C. jejuni in a viscous environment may be an important factor in the interaction of these organisms with host epithelial cells. The pH, which affects C. jejuni motility, may also influence the tropism of these organisms.

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Effect of enteroviruses on adherence to and invasion of HEp-2 cells by Campylobacter isolates

Cited by 17 publications

References 31 publications

Enhancement of Disease and Pathology by Synergy of Trichuris Suis and Campylobacter Jejuni in the Colon of Immunologically Naive Swine

Enhancement of Disease and Pathology by Synergy of Trichuris Suis and Campylobacter Jejuni in the Colon of Immunologically Naive Swine

Antibiotic Resistance Patterns of Major Zoonotic Pathogens from All-Natural, Antibiotic-Free, Pasture-Raised Broiler Flocks in the Southeastern United States

Campylobacter jejuni motility and invasion of Caco-2 cells

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