Monoterpenic aldehydes as potential anti-Leishmania agents: Activity of Cymbopogon citratus and citral on L. infantum, L. tropica and L. major

Machado, Marisa; Pires, Pedro; Dinis, Alexandra; Santos-Rosa, Manuel; Alves, Vera; Salgueiro, Lı́gia; Cavaleiro, Carlos; Sousa, Maria do Céu

doi:10.1016/j.exppara.2011.12.012

Cited by 90 publications

(53 citation statements)

References 37 publications

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“…Thus, despite of the documented toxic effect of citral to several parasites, the inhibition growth evaluation involving these oils may display some variation range effect when compared to different oils and experiments. Because of high volatility and lipophilicity of the essential oils, they are readily attached to penetrate into the cell membrane to exert their biological effect (Machado et al, 2012). Their mechanism of action appears to be predominantly on the fungal cell membrane, disrupting its structure causing leakage and cell death; blocking the membrane synthesis; inhibition of the spore germination, fungal proliferation and cellular respiration.…”

Section: Citral Rich Essential Oil Biological Activitiesmentioning

confidence: 99%

“…This fact is relevant data since in both species C. citratus and P. brevipedunculata the EO is also composed by -and -pinene followed by limonene. Hydrophobic molecules can easily diffuse across cell membranes and consequently gain advantage in what concerns to interactions with intracellular targets, being a valuable research option also for the search of new anti-Leishmania drug templates (Edris, 2007;Machado et al, 2012). Out of the 1114 strains belonging 105 sensible and resistant species of microbes (molds, yeasts and bacteria) have been already investigated front of citral rich essential oil, 38.2% were sensitive to lemongrass oil discs containing 50 μg oil/disc.…”

Section: Citral Rich Essential Oil Biological Activitiesmentioning

confidence: 99%

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Traditional use, chemical composition and antimicrobial activity of Pectis brevipedunculata essential oil: A correlated lemongrass species in Brazil

Marques

Lima

Alviano

et al. 2013

Emir. J. Food Agric

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For centuries, medicinal plants have been used as source of active principles for the treatment of many conditions. Ethnobotanical studies and bioassay guided isolation procedures have been successfully used in order to investigate and confirm their medicinal prescriptions. Traditionally used in Brazil, Cymbopogon citratus (lemongrass) is usually consumed as tea drink due to its calmative, anxyolitic as well as antihypertensive properties. Due to the similar lemongrass scent many species of Pectis genus have been used as infusion drinks for the same purposes as C. citratus. In Brazil, Pectis brevipedunculata, a sandy ornamental aromatic grass, is one of the "lemongrass odor" correlated species traditionally consumed. Chemical analysis of its essential oil was performed using GC-FID and GC-MS. Such essential oil was characterized by a high percentages of citral (81.8%: neral 35.6% and geranial 46.2%), followed by limonene (2.9%) and α-pinene (2.6%). Chemical and ethnobotanical investigations were performed involving one of the most commonly used Pectis species, known as lemongrass in order to confirm the medicinal indications compared to their chemical profile. The essential oil of P. brevipedunculata was tested against several clinical parasites. Our results were in agreement to the literature survey, suggesting the citral as the principal active constituent of the tested samples. Despite of the wide biological activities spectrum related to the major constituent presented in the essential oil of the most Pectis species, it is necessary to continue the phytochemical and pharmacological studies about the infusions constituents and validate the folk medicine.

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Section: Citral Rich Essential Oil Biological Activitiesmentioning

confidence: 99%

Section: Citral Rich Essential Oil Biological Activitiesmentioning

confidence: 99%

Traditional use, chemical composition and antimicrobial activity of Pectis brevipedunculata essential oil: A correlated lemongrass species in Brazil

Marques

Lima

Alviano

et al. 2013

Emir. J. Food Agric

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“…Essential oils and essential oil constituents have been studied, in the last years, for their activity on several pathogenic protozoan as Giardia sp. [10][11][12], Leishmania sp. [13,14], and Trypanosoma sp.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Trypanocidal Activity of the Essential Oils from Hedychium coronarium J. Koenig (Zingiberaceae)

Rodrigues

Velásquez

Cavaleiro

et al. 2013

ISRN Infectious Diseases

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The composition of the essential oils (EO) from leaves and rhizomes of Hedychium coronarium was analyzed both by gas chromatography and gas chromatography-mass spectroscopy. Thirty and thirty-nine compounds were identified, respectively, in the oils from leaves and rhizomes, representing 88% and 86.1% of the whole compositions. Caryophyllene oxide is the major component in rhizomes while 1,8-cineole predominates in leaves oil. Essential oils and major components were tested for trypanocidal activity using procyclic forms of Trypanosoma brucei (427 and 29-13 strains). The cytotoxicity index (CI 50 ), using the MTT colorimetric method, showed that essential oils and 1,8-cineole were inactive (>100 g⋅mL −1 . Nevertheless, caryophyllene oxide revealed a remarkable activity against both T. brucei strains (CI 50 = 65.77 g⋅mL −1 and 24.53 g⋅mL −1 , resp.), and the synergism between caryophyllene oxide plus pentamidine (1 : 1, v/v) highly increased the trypanocidal activity (<1.0 g⋅mL −1 ).

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“…There are only a few reports on the activity of essential oils against L. infantum (IC 50 values from 25 to 296 g/ml) (Machado et al, 2010a,b), L. tropica (IC 50 value of 52 g/ml) and L. major (IC 50 values from 29.1 to >640 g/ml) (Monzote et al, 2010;Machado et al, 2012;Sanchez-Suarez et al, 2013). Comparing these with the present data, we see that T. capitellatus oil shows antileishmanial activity that is similar or higher than that described for the other essential oils on these three Old World species.…”

Section: Discussionmentioning

confidence: 99%

Activity of Thymus capitellatus volatile extract, 1,8-cineole and borneol against Leishmania species

Machado

Dinis

Santos-Rosa

et al. 2014

Veterinary Parasitology

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a b s t r a c tIn the search for new leishmanicidal agents, Thymus capitellatus Hoffmanns. & Link (family Lamiaceae) volatile extract and its major compounds, 1,8-cineole and borneol, were tested against Leishmania infantum, Leishmania tropica and Leishmania major. Plant volatile extract (essential oil) was analysed by GC and GC-MS and the activity of essential oil on Leishmania promastigotes viability was assessed using tetrazolium-dye colorimetric method (MTT). The MTT test was also used to assess the cytotoxicity of essential oil on macrophages and bovine aortic endothelial cells. Effects on parasites were also analyzed by flow cytometry in order to assess mitochondrial transmembrane electrochemical gradient (JC-1), analyze phosphatidylserine externalization (annexin V-FITC, propidium iodide) and evaluate cell cycle (DNase-free, RNase, PI). Morphological and ultrastructural studies were performed by light, scanning and transmission electron microscopy. T. capitellatus volatile extract exhibited anti-parasite activity on Leishmania species, with IC 50 values ranging from 35 to 62 g/ml. However, major compounds 1,8-cineole and borneol did not showed biological activity suggesting that these monoterpenes are not responsible for the antileishmanial activity of T. capitellatus essential oil. Appearance of aberrant-shaped cells, mitochondrial swelling and autophagosomal structures were some of the ultrastructural alterations exhibited among treated promastigote cells. T. capitellatus promoted leishmanicidal effect by triggering a programmed cell death as evidenced by externalization of phosphatidylserine, loss of mitochondrial membrane potential, and cell-cycle arrest at the G(0)/G(1) phase. The volatile extract did not induced cytotoxic effects on mammalian cells. Taken together, these results suggest that T. capitellatus may represent a valuable source for therapeutic control of leishmaniasis in humans and animals.

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Monoterpenic aldehydes as potential anti-Leishmania agents: Activity of Cymbopogon citratus and citral on L. infantum, L. tropica and L. major

Cited by 90 publications

References 37 publications

Traditional use, chemical composition and antimicrobial activity of Pectis brevipedunculata essential oil: A correlated lemongrass species in Brazil

Traditional use, chemical composition and antimicrobial activity of Pectis brevipedunculata essential oil: A correlated lemongrass species in Brazil

Chemical Composition and Trypanocidal Activity of the Essential Oils from Hedychium coronarium J. Koenig (Zingiberaceae)

Activity of Thymus capitellatus volatile extract, 1,8-cineole and borneol against Leishmania species

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