Monospecific Antigranulocyte Sera Against Human Neutrophils, Eosinophils, Basophils, and Myeloblasts

Mahmoud, A. A. F.; Kellermeyer, RobertW.; Warren, K. S.

doi:10.1016/s0140-6736(74)90809-5

Cited by 35 publications

(11 citation statements)

References 11 publications

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“…Basophils sediment with the lymphocytes and monocytes after centrifugation on Ficoll-metrizoate; it was previously shown that this mononuclear-rich interface fraction, with or without subsequent removal of phagocytic cells with carbonyl iron, failed to damage antibody-coated schistosomula. Moreover, Butterworth and Mahmoud (unpublished observations) have found that pretreatment of unpurified buffy coat preparations with a monospecific antibasophil serum (22) and complement did not affect subsequent cytotoxicity under circumstances in which pretreatment with anti-eosinophil serum caused a 90% reduction in cytotoxicity.…”

Section: Discussionmentioning

confidence: 98%

Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purieid eosinophils.

Butterworth¹,

David²,

Franks³

et al. 1977

The Journal of Experimental Medicine

174

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After earlier observations that antibody-dependent, cell-mediated damage to 51Cr-labeled schistosomula can be ablated by pretreatment of a mixed preparation of human peripheral blood leukocytes with an anti-eosinophil serum and complement, we investigated the cytotoxic effects of eosinophil-enriched cell preparations. Preparations containing up to 98.5% eosinophils and devoid of neutrophils were effective in mediating antibody-dependent damage to schistosomula. Preparations enriched in mononuclear cells or in neutrophils, and devoid of eosinophils, were inactive. Eosinophils from some patients with eosinophilia induced by schistosomiasis were less active on a cell-to-cell basis than cells from normal individuals. The possibility that such cells were initially blocked by immune complexes was considered, and it was found that reasonable cytotoxicity by purified eosinophils from patients with eosinophilia could be generated by overnight cultures. A possible requirement for cooperation between eosinophils and other cell types was also studied. Lymphocytes, neutrophils and monocytes failed to enhance eosinophil-mediated cytotoxicity. These results provide further evidence that the eosinophil is the only cell in man responsible for antibody-dependent, complement-independent damage to schistosomula in vitro. Eosinophils from individuals, however, differ in their cytotoxic potential by a mechanism yet to be elucidated. The possible relationship of these findings to immunity in vivo is discussed.

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Section: Discussionmentioning

confidence: 98%

Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purieid eosinophils.

Butterworth¹,

David²,

Franks³

et al. 1977

The Journal of Experimental Medicine

174

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“…Comparable results have been recorded in guinea pigs treated with antineutrophil serum: the mature cells were destroyed but there was a proliferation of their precursors in the bone marrow (28). The recent development of monospecific antisera to human eosinophils, basophils, neutrophils, and myeloblasts has revealed similar specificity with respect to the stage of maturity of the cells (29,30).…”

Section: Discussionmentioning

confidence: 98%

Antieosinophil serum and the kinetics of eosinophilia in Schistosomiasis mansoni.

Mahmoud¹,

Warren²,

Graham³

1975

The Journal of Experimental Medicine

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Although eosinophilia is a hallmark ofhelminth infections, the function ofthe eosinophil granulocyte remains unknown. The recent development of a monospecific rabbit antimouse antieosinophil serum (AES)' in our laboratories (1) has provided a unique tool by which the roles of eosinophils may be elucidated . Using a murine model of schistosomiasis, the most important helminth infection of man (2), the kinetics of eosinophilia in the peripheral blood, bone marrow, and tissue lesions was carefully established; this was followed by an examination of the effects of AES on these reactions.In previous studies of the eosinophilia in schistosomiasis in mice the animals were hyperinfected, and mortality was high in the acute stages of infection (3, 4) . Mice exposed to relatively low numbers of cercariae, however, will survive for at least a year despite the development of mild to moderate hepatosplenic disease (5, 6) . The kinetics of eosinophilia in the peripheral blood and bone marrow was studied, therefore, in mice exposed to three different intensities of infection and the responses were followed for as long as 20 wk.The effect of Schistosoma mansoni eggs on eosinophilia in the blood, bone marrow and granulomatous lesions was then examined by injecting isolated purified eggs into mice singly and multiply by various routes . While the host response was dependent on the route of injection, primary and anamnestic secondary reactions were seen after successive intravenous injections. Administration of AES under these conditions profoundly suppressed peripheral eosinophilia, induced a marked increase in eosinophil precursors in the bone marrow, and ablated eosinophils from the tissue lesions, considerably reducing their size . Materials and MethodsAnimals . Young adult female mice of the CF-1 strain (18-22 g in body weight) obtained from Carworth Farms, Inc. were used in these experiments .Eosinophilia in Naturally Infected Mice . Groups of mice were infected by the subcutaneous injection of 10, 50, or 200 cercariae of the Puerto Rican strain of S . mansoni as described by Peters and Warren (7). The worm burden in each of the experimental groups was assessed by perfusion of 10 mice 8 wk after infection (8). Eosinophil counts on five animals in each group were done weekly for the first 6 wk after infection and then at 2-wk intervals.

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“…The AES contained agglutinating and cytotoxic antibodies which reacted specifically with eosinophils but did not crossreact with any other formed blood elements (13,23 (1). In the present investigation, transfer to normal mice of sera or their low molecular weight fractions from animals treated with AES induced a significant increase in the eosoinophil population.…”

Section: Discussionmentioning

confidence: 41%

“…In earlier studies, methods were developed to raise antibodies in rabbits against purified suspensions of mouse or human eosinophils (13,22,23). The AES contained agglutinating and cytotoxic antibodies which reacted specifically with eosinophils but did not crossreact with any other formed blood elements (13,23 (1).…”

Section: Discussionmentioning

confidence: 99%

Eosinophilopoietin

Mahmoud¹,

Stone²,

Kellermeyer³

1977

J. Clin. Invest.

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A B S T R A C T In earlier studies, methods were developed to raise specific antibodies in rabbits against purified suspensions of mouse or human eosinophils. On administration of antieosinophil serum (AES) to mice, the mature eosinophils in tissues, peripheral blood, and bone marrow were depleted, while the immature eosinophil pool in the bone marrow was observed to proliferate. The current investigations explore the generation of eosinophilopoietic factors during AES-induced eosinophilopenia. Mice received three injections of AES, one every other day. As the peripheral eosinophil counts started to recover after the last AES injection, the serum was collected and transferred to normal animals. Within 2 days the recipients showed an increase in peripheral blood as well as in bone marrow eosinophils. The rise in bone marrow eosinophils was due to newly formed cells as evidenced by increased uptake of [3H ]thymidine. The generation of eosinophilopoietic activity was specifically related to depletion of eosinophils but not neutrophils. The eosinophilopoietic activity was: (a) dependent on the volume of serum transferred, (b) lost on dialysis, and (c) largely heat labile. The activity eluted as a low molecular weight substance on G-25 Sephadex and was digested by pronase but not by trypsin. Active fractions collected from G-25 columns were not chemotactic for the eosinophils in vitro. Thus, specific depletion of mature eosinophils generates a low molecular weight peptide which stimulates eosinophilopoiesis in vivo. It is suggested that this substance be named eosinophilopoietin.

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Monospecific Antigranulocyte Sera Against Human Neutrophils, Eosinophils, Basophils, and Myeloblasts

Cited by 35 publications

References 11 publications

Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purieid eosinophils.

Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purieid eosinophils.

Antieosinophil serum and the kinetics of eosinophilia in Schistosomiasis mansoni.

Eosinophilopoietin

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