The early St ages of absorption of intravenously injected horseradish peroxidase iii proximal uluiles of mouse kidney were st tidied wit Ii a new tilt rast rtict ural cvi ochemical 1echziiqtie. In animals killed as early as 90 sec after inject ion, reaction product was found ott I he l)rlishborder memi)rai,es and in the apical t tibular invagi nat ions. From the l:it ter St rtict ores it \ :s5 transported to the apical vacuoles, in which it as progressively conceiit rated to form pro-I ciii al)sorpt iou droplets. The met hod, which employs 3, 3'-diamiuiobeuizidi ute as oxidizable stll)st rate, gives sharp localizat iou and is sensitive. This system is advantageous iii studying he early st ages of renal t ibtilar protein absorption, Si nec small amoiiuit 5 (If prot ciii out mem
LETTERS TO ' t1E Enm'roR nmecliumn was pipetted off, and time section flooded! wit.ii unlabehlee! medium. After 5 minutes, the proceolure is repeated. This treat memit facilitates exchange of excess labelled for unlabelled glucose-1-phosphate. Slides are then rinsed iii isotonic saline solutiomi amid transferred to 70% alcohol for several hours. Slides which were to be iodinestain controls were imicul)ateol in umidiluted labelled substrate medium and transferred dire('t.ly to 70% alcohol after a rinse in saline solution. All slides were brought. to water and washed for at least three hours. These treatments remove glucose-i-phosphate muelsorbed by time tissue section. Slides stained with dilute Lugol's iooline to cont rod the localization of pimosphorylase activity were again washed in running water to remove iodine. A few slides were stained with time periodic aciel-Schiff-dimedone reaction for glycogen (Bulmer, Stain Technol.
Autism spectrum disorder patients with autism, younger age and an additional diagnosis were more uncooperative. Factors associated with the use of GA and protective stabilization in patients with ASD were also identified.
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