Monooxygenase activities in carp as biochemical markers of pollution by polycyclic and polyhalogenated aromatic hydrocarbons: choice of substrates and effects of temperature, gender and capture stress

Machala, Miroslav; Nezveda, Karel; Petřivalský, Marek; Jarošová, Alžběta; Piačka, Vladimír; Svobodová, Zdeňka

doi:10.1016/s0166-445x(96)00824-7

Cited by 52 publications

(24 citation statements)

References 25 publications

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“…PROD in mammals is associated with cytochrome P4502B. PROD activity has been detected in several marine and freshwater fish species exposed to water-borne pollutants, including C. carpio (Machala et al, 1997), O. mykiss (Lindstrom-Seppa et al, 1992Haasch et al, 1994), and immunological detection of P4502B has been demonstrated for Stenotomus chrysops (Stegeman, 1989). To our knowledge this is the first time induction of PROD activity has been recorded in fish exposed to sediments containing complex mixtures of contaminants.…”

Section: Cytochrome P450 Inductionmentioning

confidence: 67%

Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L.

Hartl

Kilemade

Sheehan

et al. 2007

Marine Environmental Research

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Hatchery-reared turbot (Scophthalmus maximus L.) were exposed for 3 weeks, under laboratory conditions, to sediment collected from polluted sites in Cork Harbour and a reference site at Ballymacoda, Co. Cork, Ireland. The potential of surficial sediment for inducing hepatic biomarkers was assessed at two levels of biological organisation: expression of cytochrome P450 [Western blotting analysis and 7-ethoxy-resorufin O-dealkylase (EROD), 7-benzoxy resorufin O-dealkylase (BROD), 7-methoxy resorufin O-dealkylase (MROD), 7-pentoxy-resorufin O-dealkylase (PROD) activities] and DNA integrity (Comet assay). Positive controls were generated, either by exposing turbot to cadmium chloride-spiked seawater (Comet assay) or to beta-naphthaflavone by intraperitoneal injection (cytochrome P450 induction). The induction of cytochrome P450 activity (EROD, MROD and PROD) in animals following a 7-day exposure to contaminated sediments was significantly higher than those exposed to reference site sediment and remained elevated thereafter; BROD was not induced. DNA single-strand breaks were also significantly higher following exposure to contaminated sediments throughout the experiment. Although no direct correlation between induction of alkoxyresorufin O-dealkylase activities and a particular chemical class was established, the induction of MROD and PROD activities in fish exposed to sediments containing complex contaminant mixtures, appeared to be more sensitive than conventional EROD activity assays. We conclude from the present laboratory study that S. maximus is a suitable sentinel species for the assessment of moderately contaminated sediments and therefore allows for the further development of this model for future, ecologically relevant, field studies.

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Section: Cytochrome P450 Inductionmentioning

confidence: 67%

Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L.

Hartl

Kilemade

Sheehan

et al. 2007

Marine Environmental Research

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“…Exposure to metals has seemed to inhibit the CYP1A system in vertebrates [40,[45][46][47][48] and invertebrates [49][50][51] in most studies, although EROD activity has repeatedly been observed to be induced by lipophilic pollutants in vertebrates [39,52,53]. However, heavy metals are potentially both inhibitory and inductive to EROD activity [54].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of EROD and CYP3A4 activities in earthworm Eisenia fetida as biomarkers for soil heavy metal contamination

Cao

Song

Kai

et al. 2012

Journal of Hazardous Materials

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“…This may be hazardous for living systems, including specific enzymes, proteins, and genes [14,15]. There are many studies with different organisms which evaluated expression levels of various genes including metallothioneins and cytochrome after exposure to heavy metals [2,[16][17][18][19][20][21][22][23]. However, lack of knowledge how short-term exposure to zinc and cobalt affects the gene expression levels in rainbow trout introduces importance of this study.…”

Section: Introductionmentioning

confidence: 99%

Influence of Cobalt and Zinc Exposure on mRNA Expression Profiles of Metallothionein and Cytocrome P450 in Rainbow Trout

Ceyhun

Aksakal

Ekinci

et al. 2011

Biol Trace Elem Res

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The present research aims to evaluate the effects of cobalt and zinc exposure of rainbow trout (Oncorhynchus mykiss) on metallothioneins and cytocrome P450. Mature rainbow trouts were exposed to 10 mg/L CoCl(2).6H(2)O and 1 mg/L ZnSO(4).7H(2)O. After 6, 12, 24, and 48 h of treatment, expressions of muscle MT-A, MT-B, and CYP P4501A1 mRNAs were measured by means of quantitative real-time polymerase chain reaction. During the exposure experiments, no mortalities occurred. We observed that expression levels of all genes increased with exposure time. Since the organism has not learned how to completely dispose of heavy metals and tends to bioaccumulate them, our results indicate that cobalt and zinc exposure may result in accumulation of the non-eliminated metals which may lead to fish death.

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Monooxygenase activities in carp as biochemical markers of pollution by polycyclic and polyhalogenated aromatic hydrocarbons: choice of substrates and effects of temperature, gender and capture stress

Cited by 52 publications

References 25 publications

Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L.

Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L.

Evaluation of EROD and CYP3A4 activities in earthworm Eisenia fetida as biomarkers for soil heavy metal contamination

Influence of Cobalt and Zinc Exposure on mRNA Expression Profiles of Metallothionein and Cytocrome P450 in Rainbow Trout

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