Mononuclear leukocyte apoptosis in haemodialysis patients: the role of cell thiols and vitamin E

Galli, Francesco; Ghibelli, Lina; Buoncristiani, U; Bordoni, Valeria; D’Intini, Vincenzo; Benedetti, Serena; Canestrari, Franco; Ronco, Claudio; Floridi, Ardesio

doi:10.1093/ndt/gfg210

Cited by 31 publications

(26 citation statements)

References 21 publications

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“…21 Although the exact mechanisms of inflammation and oxidative stress have not been accurately elucidated in CKD patients, a number of following described factors appear to be involved, including uremic toxins, 3 rennin-angiotensin system, 22 hypertension, 23 underlying diseases (diabetes, autoimmune diseases, etc. ), 24 infection, iron overload, 25 antioxidant deficiency, 26 etc. Oxidative stress can provoke inflammation via activating NF-kB and consequent generation of pro-inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

The progress of inflammation and oxidative stress in patients with chronic kidney disease

Luo

Liu

et al. 2014

Renal Failure

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Background: The variations and their correlation of inflammation and oxidative stress in chronic kidney disease (CKD) have not been thoroughly understood. Materials and methods: Biomarkers of inflammation and oxidative stress were measured in a cohort of 176 patients with CKD ranging from stage 1 to 5 and 67 healthy controls. Correlation analysis in levels between inflammation and oxidative stress was also performed with estimated glomerular filtration rate (eGFR) using the Modification of Diet in Renal Disease (MDRD) formula. Concentrations of serum creatinine (Scr), hs-CRP (hypersensitive C reactive protein) and MDA (malondialdehyde) of these participants were measured again after 12 month follow-up. Results: In the present study, with the development of CKD, serum levels of hs-CRP, interleukin-6 (IL-6) and MDA were significantly increased, and the serum levels of SOD (superoxide dismutase) and GSH-PX (glutathione peroxidase) were significantly decreased in these participants. eGFR was inversely associated with MDA and positively with SOD and GSH-PX when adjusting for age and hypertension therapy. IL-6 and hs-CRP were positively correlated with MDA, and negatively associated with SOD and GSH-PX. Notably, after 12-month follow-up, the increase in Scr was positively associated with the increase in hs-CRP (p50.01) and MDA (p50.05), respectively. Conclusions: Inflammation and oxidative stress interacted with each other and played pivotal roles in the development of CKD. Variation in eGFR was parallel with the changes of oxidative stress and inflammation when CKD developing.

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Section: Discussionmentioning

confidence: 99%

The progress of inflammation and oxidative stress in patients with chronic kidney disease

Luo

Liu

et al. 2014

Renal Failure

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“…These unfavorable phenomena exaggerate CRF-related complications, such as atherosclerosis and associated cardiovascular complications, accelerated ageing, β 2 -microglobulin arthropathy, defective deformability of erythrocytes, cataract, anemia, increased hemolysis and platelet dysfunction [1,2,3,4,5,6]. Such oxidative stress can also accelerate the apoptosis of leukocytes in hemodialyzed (HD) patients [7]. Numerous proteins are targets of oxidative stress as well [6, 8].…”

Section: Introductionmentioning

confidence: 99%

Vitamins A, E and C as Non-Enzymatic Antioxidants and Their Relation to Lipid Peroxidation in Children with Chronic Renal Failure

Zwołińska

Grzeszczak²,

Szczepańska³

et al. 2005

Nephron Clin Pract

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Background: Increased lipid peroxidation (LP) and reduced enzymatic antioxidant defense have been observed in predialysis patients with advanced chronic renal failure (CRF) and in patients on maintenance hemodialysis (HD). To extend these observations, we evaluated the plasma, erythrocyte and dialysate levels of vitamins A and E and the plasma and dialysate levels of vitamin C as exogenous non-enzymatic antioxidants in children with CRF treated conservatively and on HD. The data obtained were related to LP monitored by erythrocyte malonyldialdehyde (E-MDA) and plasma organic hydroperoxide (OHP) concentrations. Patients: Forty-six predialysis children were enrolled in the study and divided into 2 groups: group I = moderate CRF (plasma creatinine <265.3 µmol/l), and group II = plasma creatinine ≧265.3 µmol/l. Group III consisted of 21 HD children. 27 age-matched healthy subjects served as a control group. Results: The plasma levels of vitamin A and vitamin C were significantly reduced in all CRF patients when compared to the controls, with the lowest values observed in children on maintenance HD (group III). Significant differences were also noted between the moderate CRF (group I) and HD (group III). Plasma levels of vitamin E were significantly decreased in moderate CRF (group I) and HD (group III) as compared to controls. In contrast, the erythrocyte vitamin A and vitamin E levels of predialysis children and HD patients were not different from the controls. The E-MDA and OHP concentrations in the 3 groups of CRF children were significantly higher than in healthy subjects. The concentration of plasma vitamin C was significantly inversely correlated with E-MDA, plasma OHP and creatinine in group I. In group II we found a significant correlation of plasma vitamin E levels with creatinine and E-MDA and a correlation of the plasma vitamin C concentration with E-MDA. Conclusion: CRF in children is associated with decreased concentrations of plasma antioxidant vitamins. This reduction is most expressed in children on maintenance HD and particularly concerns plasma vitamin C and erythrocyte vitamin E concentrations. The low levels of plasma vitamin A, E and C might result in reduced activity of the non-enzymatic antioxidant defense system and might be responsible for increased oxidative stress occurring in children with CRF.

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“…Antioxidants (vitamin E) have been recently used in an attempt to improve biocompatibility of dialysis [8, 9, 32, 33]. Use of vitamin E-coated dialysis membranes, have been demonstrated to reduce levels of oxidative stress in PBMCs of patients undergoing hemodialysis [32, 33].…”

Section: Discussionmentioning

confidence: 99%

“…We and other investigators previously reported that dialysis membranes activate neutrophils to generate free radicals [6, 7]. Since free radicals have been reported to induce cell injury various investigators have recommended the use of vitamin E-coated dialysis membranes [8, 9]. …”

Section: Introductionmentioning

confidence: 99%

Dialysis Membrane-Induced Oxidative Stress: Role of Heme Oxygenase-1

Bhaskaran

Radhakrishnan²,

Patni³

et al. 2006

Nephron Exp Nephrol

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Dialysis membranes have been reported to induce monocyte apoptosis. We studied the role of oxidative stress in the induction of dialysis membrane-induced monocyte apoptosis. Superoxide dismutase, a superoxide scavenger, prevented dialysis membrane-induced monocyte apoptosis. Similarly, other antioxidants also inhibited dialysis membrane- induced apoptosis. In addition, the interaction of dialysis membranes with monocytes was associated with the generation of molecules leading to oxidative stress such as superoxide and TBARS. Interestingly, pre-induction of heme oxygenase (HO)-1 by hemin prevented dialysis membrane-induced monocyte apoptosis, whereas inhibition of HO-1 activity (treatment with tin protoporphyrin, SN-P) enhanced dialysis membrane-induced monocyte apoptosis. We suggest that oxidative injury associated with dialysis membrane and monocyte interaction plays a role in monocyte injury. Pre-induction of HO-1 may attenuate dialysis membrane-induced monocyte apoptosis.

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Mononuclear leukocyte apoptosis in haemodialysis patients: the role of cell thiols and vitamin E

Cited by 31 publications

References 21 publications

The progress of inflammation and oxidative stress in patients with chronic kidney disease

The progress of inflammation and oxidative stress in patients with chronic kidney disease

Vitamins A, E and C as Non-Enzymatic Antioxidants and Their Relation to Lipid Peroxidation in Children with Chronic Renal Failure

Dialysis Membrane-Induced Oxidative Stress: Role of Heme Oxygenase-1

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