Monolithic silica columns with various skeleton sizes and through-pore sizes for capillary liquid chromatography

Motokawa, Masanori; Kobayashi, Hiroshi; Ishizuka, N.; Minakuchi, Hiroyoshi; Nakanishi, Koji; Jinnai, Hiroshi; Hosoya, Ken; Ikegami, Tohru; Tanaka, Nobuo

doi:10.1016/s0021-9673(02)00133-4

Cited by 275 publications

(222 citation statements)

References 27 publications

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“…1. It can be seen that like many previously reported unmodified silica monoliths [37,38], the dynamically coated silica monolith is also characterized by a continuous skeleton and large through-pores, and the large through-pores can provide good permeability with low backpressure. Compared to an unmodified silica monolith, no difference in permeability was observed here.…”

Section: Characterization Of the [Bmim][bf 4 ] Dynamically Coated Silsupporting

confidence: 66%

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Dynamically coated silica monolith with ionic liquids for capillary electrochromatography

et al. 2008

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An ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM] [BF 4 ]) was introduced as dynamic coating of a silica monolithic column for capillary electrochromatography of phenols and nucleoside monophosphates. The run-to-run and column-to-column repeatability of migration time for six phenols were satisfactory on this column with relative standard deviation values less than 0.90 and 4.31%, respectively. Anodic electroosmotic flow (EOF) was observed, which increased with the increase of [BMIM] [BF 4 ] on the monolith. Efficient separation of phenols and nucleoside monophosphates on this dynamically coated monolithic column was obtained, compared with a dynamically coated fused-silica column and unmodified silica monolithic column. The retention behavior of uncharged phenols is mainly manipulated by hydrophobic interactions due to the presence of butyl groups, and that of nucleoside monophosphates is governed by the electrostatic attraction mechanism based on the interaction between positively charged [BMIM] [BF 4 ] moieties and negatively charged phosphate groups. In addition, silica matrix also contributes to the separation resolution.

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Section: Characterization Of the [Bmim][bf 4 ] Dynamically Coated Silsupporting

confidence: 66%

“…It was dried under nitrogen at 1207C in a GC oven for 2 h. The monolithic silica column was prepared as described by Tanaka et al [37] with modifications. Briefly, 0.1 g PEG (M w 10 000), 1 mL 0.01 M acetic acid, and 0.4 mL TMOS were mixed together.…”

Section: Preparation Of Monolithic Silica Columnsmentioning

confidence: 99%

Dynamically coated silica monolith with ionic liquids for capillary electrochromatography

et al. 2008

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“…As shown in Fig. 3, trialkoxysilanes with hydrophobic organic moieties, such as N-octadecyldimethyl [3-(trimethoxy silyl)propyl]ammonium chloride [23], n-octyltriethoxysilane (C8-TES) [32][33][34][35], phenyltriethoxysilane (PTES) [36][37][38], propyltrimethoxysilane (C3-TMS) [39], MTMS [40][41][42][43][44], and other trialkoxysilanes with active organic functionalities, such as aminopropyl triethoxysilane (APTES) [45][46][47][48][49], N-(␤-aminoethyl)-␥-aminopropyl-triethoxysilane [50], N-(␤-aminoethyl)-␥-aminopropylmethyldimethoxysilane [51], 3-chloropropyltrimethoxysilane [52], 3-mercaptopropyltrime thoxysilane (MPTMS) [53], N-trimethoxysilylpropyl-N,N,Ntrimethylammonium chloride [54], allyl-trimethoxysilane [ 55,56], and vinyltrimethoxysilane (VTMS) [57] have been selected as coprecursors with tetraalkoxysilanes, TMOS, or TEOS. Additionally, bridged silanes such as 1,4-bis(triethoxysily1)benzene [58] have also been used for preparation of hybrid monoliths.…”

Section: Sol-gel Process Of Trialkoxysilanes and Tetraalkoxysilanesmentioning

confidence: 99%

Recent advances in preparation and application of hybrid organic‐silica monolithic capillary columns

Lin

Zhang

et al. 2012

Electrophoresis

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Hybrid organic-silica monolithic columns, regarded as a second generation of silica-based monoliths, have received much interest due to their unique properties over the pure silicabased monoliths. This review mainly focuses on development in the fields of preparation of hybrid monolithic columns in a capillary and their application for CEC and capillary liquid chromatography separation, as well as for sample pretreatment of solid-phase microextraction and immobilized enzyme reactor since July 2010. The preparation approaches are comprehensively summarized with three routes: (i) general sol-gel process using trialkoxysilanes and tetraalkoxysilanes as coprecursors; (ii) "one-pot" process of alkoxysilanes and organic monomers concomitantly proceeding sol-gel chemistry and free radical polymerization; and (iii) other polymerization approaches of organic monomers containing silanes. The modification of hybrid monoliths containing reactive groups to acquire the desired surface functionality is also described.

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“…The overall separation efficiencies of chromatographic columns can be significantly enhanced through the combination of very small particles with increased column length, leading to the use of ultrahigh pressures in microcolumn LC [24][25][26][27]. Finally, the properties of monolithic capillary columns, operating in either the electromigration mode (CEC) [28][29][30][31][32][33][34] or a pressure-driven mode [35,36] for the separations of both peptides and oligosaccharides, provide an additional route to solving the problems of peak overlap in proteomic and glycomic studies.…”

Section: Chromatographic Approaches To Glycoconjugate Analysismentioning

confidence: 99%

New hyphenated methodologies in high‐sensitivity glycoprotein analysis

Novotný

Mechref

2005

J of Separation Science

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High-sensitivity glycoprotein analyses are of particular interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of new hyphenated methodologies in high-sensitivity glycoprotein analysis is highlighted in this thematic review. These methodologies include, in particular, capillary LC/MALDI/TOF/TOF MS in conjunction with online permethylation platform, and silica-based lectin microcolumns interfaced to MS. The potential of these methodologies in glycomic and glycoproteomic analysis is demonstrated for model glycoproteins as well as total glycomes and glycoproteomes derived from biological samples. Additionally, the applications of CE-MS, CEC, and nanoLC with graphitized carbon in the areas of glycomics and glycoproteomics are described.

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Monolithic silica columns with various skeleton sizes and through-pore sizes for capillary liquid chromatography

Cited by 275 publications

References 27 publications

Dynamically coated silica monolith with ionic liquids for capillary electrochromatography

Dynamically coated silica monolith with ionic liquids for capillary electrochromatography

Recent advances in preparation and application of hybrid organic‐silica monolithic capillary columns

New hyphenated methodologies in high‐sensitivity glycoprotein analysis

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