New hyphenated methodologies in high‐sensitivity glycoprotein analysis

Novotný, Miloš V.; Mechref, Yehia

doi:10.1002/jssc.200500258

Cited by 89 publications

(84 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although LC/MS analysis of permethylated glycans has been reported [20,21,32], until this report, LC/MS isomer separation of these compounds has not been demonstrated. The capacity to separate permethylated oligosaccharide isomers by LC before infusion into the mass spectrometer can make their analysis more facile.…”

mentioning

confidence: 83%

See 1 more Smart Citation

A glycomics platform for the analysis of permethylated oligosaccharide alditols

Costello

Contado-Miller

Cipollo

2007

J. Am. Soc. Mass Spectrom.

156

166

View full text Add to dashboard Cite

This communication reports the development of an LC/MS platform for the analysis of permethylated oligosaccharide alditols that, for the first time, demonstrates routine online oligosaccharide isomer separation of these compounds before introduction into the mass spectrometer. The method leverages a high-resolution liquid chromatography system with the superior fragmentation pattern characteristics of permethylated oligosaccharide alditols that are dissociated under low-energy collision conditions using quadrupole orthogonal time-offlight (QoTOF) instrumentation and up to pseudo MS 3 mass spectrometry. Glycoforms, including isomers, are readily identified and their structures assigned. The isomer-specific spectra include highly informative cross-ring and elimination fragments, branch position specific signatures, and glycosidic bond fragments, thus facilitating linkage, branch, and sequence assignment. The method is sensitive and can be applied using as little as 40 fmol of derivatized oligosaccharide. Because permethylation renders oligosaccharides nearly chemically equivalent in the mass spectrometer, the method is semiquantitative and, in this regard, is comparable to methods reported using high field NMR and capillary electrophoresis. In this postgenomic age, the importance of glycosylation in biological processes has become clear. The nature of many of the important questions in glycomics is such that sample material is often extremely limited, thus necessitating the development of highly sensitive methods for rigorous structural assignment of the oligosaccharides in complex mixtures. The glycomics platform presented here fulfills these criteria and should lead to more facile glycomics analyses. (J Am

show abstract

mentioning

confidence: 83%

“…This, in part, may be due to their lack of a chromophore and the consequent barrier to off-line methods development. LC/MS methods have been reported for analysis of permethylated derivatives using reversed-phase chromatography [20,21], but improvements on these methods are desired, to provide isomer separation.…”

mentioning

confidence: 99%

A glycomics platform for the analysis of permethylated oligosaccharide alditols

Costello

Contado-Miller

Cipollo

2007

J. Am. Soc. Mass Spectrom.

156

166

View full text Add to dashboard Cite

show abstract

“…Subsequent electrospray ionization-MS screening for the occurrence of peptides containing N-terminal pyroglutamate residues was performed to identify the initial lipid-modified glutamic acid residues (348). For a general overview and detailed description of new, highly sensitive MS methods, as well as affinity chromatography-based posttranslational modification trapping methods (including those used for protein glycosylation and glycation analysis), the reader is referred to excellent reviews on this topic (192,193,220,225,262,265,290,441).…”

Section: Pargsmentioning

confidence: 99%

Nuclear ADP-Ribosylation Reactions in Mammalian Cells: Where Are We Today and Where Are We Going?

Hassa

Haenni

Elser

et al. 2006

Microbiol Mol Biol Rev

629

644

View full text Add to dashboard Cite

SUMMARY Since poly-ADP ribose was discovered over 40 years ago, there has been significant progress in research into the biology of mono- and poly-ADP-ribosylation reactions. During the last decade, it became clear that ADP-ribosylation reactions play important roles in a wide range of physiological and pathophysiological processes, including inter- and intracellular signaling, transcriptional regulation, DNA repair pathways and maintenance of genomic stability, telomere dynamics, cell differentiation and proliferation, and necrosis and apoptosis. ADP-ribosylation reactions are phylogenetically ancient and can be classified into four major groups: mono-ADP-ribosylation, poly-ADP-ribosylation, ADP-ribose cyclization, and formation of O-acetyl-ADP-ribose. In the human genome, more than 30 different genes coding for enzymes associated with distinct ADP-ribosylation activities have been identified. This review highlights the recent advances in the rapidly growing field of nuclear mono-ADP-ribosylation and poly-ADP-ribosylation reactions and the distinct ADP-ribosylating enzyme families involved in these processes, including the proposed family of novel poly-ADP-ribose polymerase-like mono-ADP-ribose transferases and the potential mono-ADP-ribosylation activities of the sirtuin family of NAD+-dependent histone deacetylases. A special focus is placed on the known roles of distinct mono- and poly-ADP-ribosylation reactions in physiological processes, such as mitosis, cellular differentiation and proliferation, telomere dynamics, and aging, as well as “programmed necrosis” (i.e., high-mobility-group protein B1 release) and apoptosis (i.e., apoptosis-inducing factor shuttling). The proposed molecular mechanisms involved in these processes, such as signaling, chromatin modification (i.e., “histone code”), and remodeling of chromatin structure (i.e., DNA damage response, transcriptional regulation, and insulator function), are described. A potential cross talk between nuclear ADP-ribosylation processes and other NAD+-dependent pathways is discussed.

show abstract

“…The occurrence of glycosylation on eukaryotic proteins is widespread; moreover, glycoproteins are distributed on different parts of living cells and in various biological fluids [14]. The structural properties of glycoproteins can be dramatically changed upon alteration of the expression of the enzymes responsible for the formation of glycosidic bonds.…”

Section: Analysis Of Glycans From Glycoproteinsmentioning

confidence: 99%

“…The informative power of ESI and MALDI-MS is increased by on-line or off-line interfacing with separative techniques. LC, CEC and CE, coupled with ESI-MS (on-line or off-line) or MALDI-MS (off-line), have been successfully used for the achievement of detailed structural information on carbohydrates in complex (even isomeric) mixtures; a strong contribution has been given by the use of modern mass analyzers such as IT, quadrupole-TOF (Q-TOF), TOF-TOF or Fourier transform ion cyclotron resonance (FT-ICR), which can provide highly informative fragmentation spectra of oligosaccharides [11,12,[14][15][16][17][18]. Notwithstanding the usefulness of MS detection, the complexity of carbohydrate-containing samples often requires a careful choice of the separative method, which need to be optimized for each given problem, and should be compared with complementary separative approaches.…”

Section: Introductionmentioning

confidence: 99%

Overview on advances in capillary electrophoresis‐mass spectrometry of carbohydrates: A tabulated review

et al. 2006

View full text Add to dashboard Cite

Overview on advances in capillary electrophoresis-mass spectrometry of carbohydrates: A tabulated reviewThe increasing interest for carbohydrates as holder of essential bioinformations has boosted their full characterization through analytical techniques. The intent of this review is to summarize the recent trends regarding on-line and off-line CE-MS coupling for carbohydrate analysis. A statistical survey on the articles that use derivatizing agents as well as on the analyzer and type of instrument coupling (i.e. on-or off-line) is depicted. From a general overview it can be concluded that, whereas derivatization might be useful for the detection of neutral carbohydrates improving separation selectivity with volatile buffers and increasing sensitivity of the MS detection, relatively few works with derivatized carbohydrates were found; this was noticed in particular for glycosides and saccharides carrying ionizable groups, which are normally analyzed without any chemical modification. The most applied coupling is the on-line sheath-liquid interface; for online applications, ESI is the sole source used, whilst the most common analyzer is the IT. MS n is often exploited, as fragmentation increases the achieved structural information. CE-MS turned out to be mainly used for the analysis of carbohydrates in drug development (i.e. study of oligosaccharides from pathogens, carbohydrate-based drugs and drug metabolites), in nutrition and for characterization of glycans from glycoproteins. The reader will find elucidating tables regarding these recent CE-MS applications, including the main information on the analysis conditions. Comments are meant to help the immediate focus on the usefulness of the analytical technique and predict the difficulties found during analysis and, in case, their overcoming.

show abstract

New hyphenated methodologies in high‐sensitivity glycoprotein analysis

Cited by 89 publications

References 102 publications

A glycomics platform for the analysis of permethylated oligosaccharide alditols

A glycomics platform for the analysis of permethylated oligosaccharide alditols

Nuclear ADP-Ribosylation Reactions in Mammalian Cells: Where Are We Today and Where Are We Going?

Overview on advances in capillary electrophoresis‐mass spectrometry of carbohydrates: A tabulated review

Contact Info

Product

Resources

About