Monolithic poly (SPE-co-BVPE) capillary columns as a novel hydrophilic interaction liquid chromatography stationary phase for the separation of polar analytes

Foo, Hsiao Ching; Heaton, James; Smith, Norman W.; Stanley, Shawn M.R.

doi:10.1016/j.talanta.2012.07.083

Cited by 14 publications

(6 citation statements)

References 11 publications

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“…Zwitterionic phases exhibit great potential for the separation of polar analytes since they offer the advantage of weak electrostatic interactions between charged analytes and zwitterionic functional groups combined with the high efficiency and selectivity of hydrophilic interactions [60]. Polar sulfoalkylbetaine monomers, possessing both positively charged quaternary ammonium and negatively charged sulfonic groups, have been widely used in the preparation of zwitterionic monolithic columns to be used in HILIC/HI-CEC applications [43, 49, 60, 61]. …”

Section: Polar Organic Monoliths For Hilic Separationsmentioning

confidence: 99%

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Recent advances in nonpolar and polar organic monoliths for HPLC and CEC

2014

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This article is aimed at providing a review of the progress made in the field over the period 2011 to present in order to expand in parts on two previous reviews (S. Karenga and Z. El Rassi, Electrophoresis, 2011, 32, 90-104; D. Gunasena and Z. El Rassi, Electrophoresis, 2012, 33, 251-261). In brief, this review article describes progress made in nonpolar and polar monoliths used in reversed phase HPLC and CEC (RPC/RP-CEC) and in hydrophilic interaction liquid chromatography/CEC (HILIC/HI-CEC), respectively. This article is by no means an exhaustive review of the literature; it is rather a survey of the recent progress made in the field with 69 references published on nonpolar and polar polymeric monoliths.

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Section: Polar Organic Monoliths For Hilic Separationsmentioning

confidence: 99%

“…Another zwitterionic monolith has been reported by Foo et al [61]. It was prepared by the co-polymerization of the zwitterionic functional monomer SPE and the crosslinker 1,2-bis(p-vinylphenyl) ethane (BVPE) in the presence of porogens toluene and methanol.…”

Section: Polar Organic Monoliths For Hilic Separationsmentioning

confidence: 99%

Recent advances in nonpolar and polar organic monoliths for HPLC and CEC

2014

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“…Polymer‐based zwitterion monoliths were prepared by copolymerization of sulfobetaine monomer with various crosslinkers and an effect of mobile phase composition and temperature on the retention has been tested . Monolithic capillary columns with sulfobetaine functionality can be successfully used in two‐dimensional liquid chromatography .…”

Section: Introductionmentioning

confidence: 99%

Controlling selectivity of polymer-based monolithic stationary phases

2019

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In this work, we aimed to prepare a monolithic capillary column that allowed an isocratic separation of ten dopamine precursors and metabolites in a single run. Segments of five zwitterion sulfobetaine polymer monoliths have been modified by zwitterion phoshorylcholine by using an ultraviolet‐initiated two‐step photografting. Columns with 0, 33, 50, 66, and 100% of modified length were prepared. Effect of length of the modified segment and mobile phase composition has been tested. All columns provided dual‐retention mechanism with reversed‐phase retention in highly aqueous mobile phase and hydrophilic interaction mechanism in highly organic mobile phase. The retention mechanism was controlled by the composition of the mobile phase and has been described by a three‐parameter model. We have used regression parameters to characterize the retention of analyzed compounds and to study individual pathways of dopamine metabolism. Comprehensive optimization of mobile phase composition allowed to find an optimal composition of the mobile phase and stationary phase surface chemistry arrangement to achieve desired separation. Optimized columns provided an isocratic separation of all tested compounds in less than nine min.

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“…The chemistry of crosslinking monomers is an important parameter affecting the properties of monolithic columns prepared using single‐step polymerization procedures . Recently, we prepared monolithic columns from nonpolar lauryl methacrylate monomer and a relatively polar tetraoxyethylene dimethacrylate crosslinker, which provided 70 000 theoretical plates/m and allowed fast isocratic separations of low‐molecular‐weight compounds with excellent column‐to‐column reproducibility .…”

Section: Introductionmentioning

confidence: 99%

“…A capillary zwitterionic monolithic column prepared by thermal copolymerization of ethylene glycol dimethacrylate (EDMA) and 2‐methacryloyloxyethylphosphorylcholine showed good selectivities for small peptides . Hydrophilic monolithic columns prepared by thermal copolymerization of zwitterionic sulfobetaines and N , N ′‐methylenebisacrylamide , or 1,2‐bis( p ‐vinylphenyl)ethane , showed a dual HILIC/RP separation mechanism and enabled fast separation of pyrimidine and purine nucleic acid bases. Viklund et al prepared monolithic capillary columns for cation‐exchange LC of proteins by EDMA and N , N ‐dimethyl‐ N ‐methacryloxyethyl‐ N ‐(3‐sulfopropyl)ammonium betaine (MEDSA) inside fused‐silica capillaries.…”

Section: Introductionmentioning

confidence: 99%

New zwitterionic polymethacrylate monolithic columns for one‐ and two‐dimensional microliquid chromatography

Jandera

Staňková

Hájek

2013

J of Separation Science

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We prepared 0.53 and 0.32 mm id monolithic microcolumns by in situ copolymerization of a zwitterionic sulfobetaine functional monomer with bisphenol A glycerolate dimethacrylate (BIGDMA) and dioxyethylene dimetacrylate crosslinkers. The columns show a dual retention mechanism (hydrophilic-interaction mode) in acetonitrile-rich mobile phases and RP in highly aqueous mobile phases. The new 0.53 mm id columns provided excellent reproducibility, retention, and separation selectivity for phenolic acids and flavonoids. The new zwitterionic monolithic columns are highly orthogonal, with respect to alkyl silica stationary phases, not only in the hydrophilic-interaction mode but also in the RP mode. The optimized monolithic zwitterionic microcolumn of 0.53 mm id was employed in the first dimension, either in the aqueous normal-phase or in the RP mode, coupled with a short nonpolar core-shell column in the second dimension, for comprehensive 2D LC separations of phenolic and flavonoid compounds. When the 2D setup with the sulfobetaine-BIGDMA column was used for repeated sample analysis, with alternating gradients of decreasing (hydrophilic-interaction mode), and increasing (RP mode) concentration of acetonitrile on the sulfobetaine-BIGDMA column in the first dimension, useful complementary information on the sample could be obtained.

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Monolithic poly (SPE-co-BVPE) capillary columns as a novel hydrophilic interaction liquid chromatography stationary phase for the separation of polar analytes

Cited by 14 publications

References 11 publications

Recent advances in nonpolar and polar organic monoliths for HPLC and CEC

Recent advances in nonpolar and polar organic monoliths for HPLC and CEC

Controlling selectivity of polymer-based monolithic stationary phases

New zwitterionic polymethacrylate monolithic columns for one‐ and two‐dimensional microliquid chromatography

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