Monolithic chromatography on conjoint liquid chromatography columns for speciation of platinum-based chemotherapeutics in serum of cancer patients

Binding Kinetics of Ruthenium Pyrithione Chemotherapeutic Candidates to Human Serum Proteins Studied by HPLC-ICP-MS

“…The eluents from regeneration and equilibration steps (flow rate 6 mL/min) were directed to waste through a software-controlled six-port valve. After approximately 30 serum separations, the CLC column was dismantled and protein G disk and DEAE disk were cleaned separately [35].…”

“…Since different types of monolithic supports are available as disks, they can be assembled together into conjoint liquid chromatography (CLC) column, which widens options of their use in speciation analysis [34][35][36][37]. In our group, this analytical approach has been applied in investigations of the kinetics of Pt-based chemotherapeutics [34,35] and Ru-based drug candidates [36] in human serum, and in speciation analyses of serum of cancer patients treated with cisplatin or carboplatin [35]. In these studies, convective interaction media (CIM) protein G and anion-exchange diethylamino (DEAE) monolithic disks were placed in one housing, forming conjoint liquid chromatography (CLC) monolithic column, which enabled two-dimensional separation in a single chromatographic run.…”

Atomic Spectrometry Update: review of advances in elemental speciation

“…A paper on the use of monolithic chromatography utilising convective interaction media (CIM) disks for Pt-drug speciation in human samples investigated the cisplatin, carboplatin and oxaliplatin protein-adducts formed in spiked and real human serum samples from patients being treated for cancer. 162 This followed on from work covered in last year’s Elemental Speciation ASU 1 for the speciation of carboplatin-protein adducts in spiked serum. The same affinity protein G and weak anion exchange diethylamine (DEAE) disks were used in series, highlighting the applicability of this approach for the investigation of a range of Pt-drugs with different chemical properties.…”

Speciation of copper in human serum using conjoint liquid chromatography on short-bed monolithic disks with UV and post column ID-ICP-MS detection

“…To this end, in our group, the speciation of Pt-and Ru-based chemotherapeutics in human serum and the serum of cancer patients was performed using a set-up that comprises convective interaction media (CIM) affinity (Protein G) and weak anion-exchange (diethylaminoethyl DEAE) disks assembled into a single housing, forming a CLC monolithic column. [31][32][33][34] The development of the albumin-depletion (α-HSA) monolithic columns with immobilized antibodies 35,36 provides the possibility to Please do not adjust margins Please do not adjust margins selectively retain the HSA prior to the separation of Cp and other human serum proteins on an anion-exchange column. The main aim of our work was to develop a new analytical method for the speciation of Cu in human serum that is based on the rapid 2D chromatographic separation of Cu-LMM, Cu bound to Cp and Cu bound to HSA.…”