Monodisperse Poly (Styrene-co-Divinylbenzene) Particles (3.2 ?m) with Relatively Small Pore Size as HPLC Packing Material

Unsal, Ender; Camli, Sevket Tolga; Irmak, Taşkın; Tuncel, Mürvet; Tuncel, Alï

doi:10.1365/s10337-004-0416-4

Cited by 19 publications

(19 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The liquid chromatograms were recorded using a UV detector operated at 254 nm at different flow rates between 0.5 and 1.5 mL/min. The resolution of successive peaks, the retention factor and the theoretical plate number (TPN) were calculated based on the conventional expressions given elsewhere [26,31,32].…”

Section: Chromatographymentioning

confidence: 99%

Monodisperse porous poly(vinyl acetate-co-divinylbenzene) particles by single-stage seeded polymerization: A packing material for reversed phase HPLC

et al. 2006

View full text Add to dashboard Cite

A single-stage swelling and polymerization method was proposed for the synthesis of monodisperse porous poly(vinyl acetate-co-divinylbenzene) [poly(VAc-co-DVB)] particles with different VAc/DVB feed ratios. The particles obtained with the VAc/DVB feed ratio of 50:50 v/v had a narrow pore size distribution exhibiting a sharp peak at 30 nm. Based on this distribution the mean pore size and the specific volume were determined as 12 nm and 1.39 mL/g, respectively. The specific surface area of poly(VAc-co-DVB) particles was found to be 470 m2/g. These properties make poly(VAc-co-DVB) particles a promising support for potential HPLC applications. Poly(vinyl alcohol-co-divinylbenzene) [poly(VA-co-DVB)] particles were then obtained by the basic hydrolysis of poly(VAc-co-DVB) particles. The hydroxyl groups on poly(VA-co-DVB) particles have a suitably reactive functionality for surface grafting or derivatization protocols aiming at synthesizing various HPLC packings. The examination of poly(VA-co-DVB) particles by confocal laser scanning microscopy showed the homogeneous distribution of hydroxyl functionality in the particle interior. As a starting point, the chromatographic performance of plain material, poly(VAc-co-DVB) particles produced with VAc/DVB feed ratio of 50:50 (v/v) was tested by a commonly utilized chromatographic mode, reversed phase chromatography. Poly(VAc-co-DVB) particles were successfully used as packing material in the RP separation of alkylbenzenes with resolutions higher than 1.5. Theoretical plate numbers up to 17 500 plates/m were achieved. No significant change both in the chromatographic resolution and column efficiency was observed with increasing flow rate. The chromatography showed that poly(VAc-co-DVB) particles were a suitable starting material for the synthesis of chromatographic packings for different modes of HPLC.

show abstract

Section: Chromatographymentioning

confidence: 99%

Monodisperse porous poly(vinyl acetate-co-divinylbenzene) particles by single-stage seeded polymerization: A packing material for reversed phase HPLC

et al. 2006

View full text Add to dashboard Cite

show abstract

“…The retention factor increased linearly with increasing number of alkyl-carbon atom. The slope of the graph is an important parameter for the evaluation of polarity and separation behavior of the column [6]. The slopes between 0.10 and 0.19 were obtained with different ACN concentrations, and the slope increased with increasing ACN concentration.…”

Section: Chromatographymentioning

confidence: 99%

“…Poly(dihydroxypropyl methacrylate-co-ethylene dimethacrylate), poly-(DHPM-co-EDM), particles obtained by the acidic hydrolysis of poly(GMAco-EDMA) particles were successfully used as column-packing material in the reversed-phase separation of alkylbenzenes [5]. Monodisperse poly(styrene-co-divinylbenzene) particles with relatively small pore size were used for separation of alkyl benzenes [6]. Similar particles were also used as stationary phase for the reversed-phase separation of proteins with high-resolution and high-column efficiency [7].…”

Section: Introductionmentioning

confidence: 99%

A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

Çelebі

2017

Acta Chromatographica

View full text Add to dashboard Cite

Summary. New polyacrylate-based monosized-porous polymer beads were proposed as a stationary phase for the separation of polar compounds by microbore reversed-phase chromatography. For this purpose, monosized-porous poly(glycerol dimethacrylate-coglycerol-1,3-diglycerolate diacrylate), poly(GDMA-co-GDGDA), beads with hydroxyl functionality were synthesized by a modified seeded polymerization. The selected octadecylating agent, stearoyl chloride (SC), was covalently attached onto the hydrophilic beads via a direct, single stage reaction with a simple synthetic route. SC attachedpoly(GDMA-co-GDGDA) beads were slurry-packed into the microbore columns and used as separation medium microbore reversed-phase chromatography. The stationary phase was used for separation of alkylbenzenes and polar analytes by micro reversedphase chromatography, using mobile phases with low acetonitrile content. Theoretical plate number (TPN) values up to 12,000 plates m −1 and 10,000 plates m −1 for alkylbenzenes and polar analytes, respectively, were achieved. The results also showed that poly(GDMA-co-GDGDA) hydrogel beads are a promising starting material for a number of chromatographic applications like reversed-phase (RP) chromatography, hydrophilic interaction chromatography (HILIC), ion-exchange chromatography (IEC), and affinity chromatography with a single-stage surface modification.

show abstract

“…The monodisperse polystyrene (PS) seed particles in 2.4 m particle size were prepared by the dispersion polymerization of styrene [22,23]. The seeded polymerization method used for the synthesis of monodisperse-macroporous particles from seed latex is given in Fig.…”

Section: Synthese Of Monodisperse-macroporous Particlesmentioning

confidence: 99%

“…The seeded polymerization method used for the synthesis of monodisperse-macroporous particles from seed latex is given in Fig. 2 [22,23]. In a typical synthesis of monodisperse porous particles, a diluent mixture containing 2 ml toluene, 1 ml DBP and 0.25 ml dodecanol was emulsified in the aqueous medium (40 ml) containing 0.25% (w/w) SDS as the emulsifier.…”

Section: Synthese Of Monodisperse-macroporous Particlesmentioning

confidence: 99%

Synthese and characterization of boronic acid functionalized macroporous uniform poly(4-chloromethylstyrene-co-divinylbenzene) particles and its use in the isolation of antioxidant compounds from plant extracts

Çetinkaya

Duru

Çiçek

2012

Journal of Chromatography B

View full text Add to dashboard Cite

Monodisperse Poly (Styrene-co-Divinylbenzene) Particles (3.2 ?m) with Relatively Small Pore Size as HPLC Packing Material

Cited by 19 publications

References 27 publications

Monodisperse porous poly(vinyl acetate-co-divinylbenzene) particles by single-stage seeded polymerization: A packing material for reversed phase HPLC

Monodisperse porous poly(vinyl acetate-co-divinylbenzene) particles by single-stage seeded polymerization: A packing material for reversed phase HPLC

A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

Synthese and characterization of boronic acid functionalized macroporous uniform poly(4-chloromethylstyrene-co-divinylbenzene) particles and its use in the isolation of antioxidant compounds from plant extracts

Contact Info

Product

Resources

About