Monocytes recruited into the alveolar air space of mice show a monocytic phenotype but upregulate CD14

Maus, Ulrich A.; Herold, Susanne; Muth, Heidrun; Maus, Regina; Ermert, Leander; Ermert, Monika; Weißmann, Norbert; Rosseau, Simone; Seeger, Werner; Grimminger, Friedrich; Lohmeyer, Jürgen

doi:10.1152/ajplung.2001.280.1.l58

Cited by 125 publications

(147 citation statements)

References 29 publications

Supporting

Mentioning

125

Contrasting

Unclassified

Order By: Relevance

“…58 Lung injury is also reduced when animals deficient in CD14, a cell surface receptor that is critical to the innate immune response and is an important receptor for LPS, are used as donors. 99 These results are consistent with the report that monocytes recruited to an inflamed lung upregulate CD14 expression and show enhanced sensitivity to LPS stimulation 100 and with the clinical observation that components of the LPS activating system are elevated in the BAL fluid of SCT patients with IPS. 1 Collectively, these data demonstrate that donor macrophages/monocytes cells are recruited to the lungs of allogeneic SCT recipients, and their ability to secrete TNFa in response to LPS stimulation directly correlates with IPS severity.…”

Section: The Role Of Donor Accessory Cellssupporting

confidence: 88%

Acute lung injury after allogeneic stem cell transplantation: is the lung a target of acute graft-versus-host disease?

Cooke

Yanik

2004

Bone Marrow Transplant

101

View full text Add to dashboard Cite

Summary:Allogeneic hematopoietic stem cell transplantation (SCT) is an important therapeutic option for a number of malignant and nonmalignant conditions but the broader application of this treatment strategy is limited by several side effects. In particular, diffuse lung injury is a major complication of SCT that responds poorly to standard therapeutic approaches and significantly contributes to transplant-related morbidity and mortality. Historically, approximately 50% of all pneumonias seen after SCT have been secondary to infection, but the judicious use of broad-spectrum antimicrobial prophylaxis in recent years has tipped the balance of pulmonary complications from infectious to noninfectious causes. This mini review will discuss the definition, risk factors and pathogeneses of noninfectious lung injury that occurs early after allogeneic SCT.

show abstract

Section: The Role Of Donor Accessory Cellssupporting

confidence: 88%

Acute lung injury after allogeneic stem cell transplantation: is the lung a target of acute graft-versus-host disease?

Cooke

Yanik

2004

Bone Marrow Transplant

101

View full text Add to dashboard Cite

show abstract

“…One of them may be a change in the ability of the interaction with LPS during the transformation of monocytes into macrophages [10]. Similar results were reported for murine recruited alveolar monocytes and resident macrophages [11].…”

Section: Discussionsupporting

confidence: 77%

Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Sládek¹,

Rysanek²,

Faldyna

2002

Vet. Res.

View full text Add to dashboard Cite

-The object of the study was the comparative assessment of phagocyte activation during initiation and resolution of mammary gland injury induced by lipopolysaccharide (LPS) or buffered salt solution (PBS) on the basis of the CD14 receptor positivity. The experiments were carried out in 15 clinically normal Holstein × Bohemian Red Pied crossbred heifers, aged 14 to 18 months. Noninflammatory and inflammatory mammary gland injury were induced by intramammary administration of PBS (10 mL) and LPS (10 mL, 1 µg/mL), respectively. Samples of the cell populations were obtained by mammary lavages at 24 h intervals. Flow cytometry was used to determine the CD14+ neutrophils, monocytes, and macrophages. The percentage of CD14+ neutrophils was only 1.2% and 1.3% 24 h after the treatment with PBS and LPS, respectively. The resolution was accompanied by an increase in proportion of CD14+ neutrophils. The proportion of CD14+ neutrophils returned to initial values in the PBS-treated, but not in the LPS-treated mammary glands till 96 h. Percentage of CD14+ monocytes increased after 24 h and the effect was more pronounced in the LPS-treated than in the PBS treated mammary glands (P < 0.05). The percentage of CD14+ macrophages decreased highly significantly at 24 h in the LPS-treated, but not in the PBS-treated mammary glands (P < 0.01). The resolution of mammary gland injury (48 to 96 h) was characterised by an increase in CD14+ macrophages proportion, which was greater in the LPS-treated than PBS-treated mammary glands (P < 0.01). The activation of macrophages during resolution of mammary gland injury can be interpreted as an important mechanism of restitution. Résumé -Activation des phagocytes au cours de l'initiation et de la résolution de lésion de la glande mammaire induite par des lipopolysaccharides chez des génisses. L'objet de la présente étude est l'analyse de l'activation des phagocytes au cours de l'initiation et de la résolution de lésion de la glande mammaire induite par des lipopolysaccharides (LPS), ou par la solution physiologique (PBS), et cela sur la base de la positivité du CD14 récepteur. L'essai fut réalisé sur 15 génisses, cliniquement en bonne santé, issues du croisement des races Holstein × Pie rouge tchèque, âgées de 14 à 18 mois. Des lésions non-inflammatoires et inflammatoires de la glande mammaire ont été induites par l'administration intra-mammaire de PBS (10 mL) et LPS (10 mL, 1 µg/mL), respectivement. Les échantillons de populations cellulaires ont été obtenus par lavage des glandes mammaires, à des intervalles de 24 heures. On a utilisé la cytométrie de flux pour détecter les neutrophiles, les monocytes et les macrophages CD14+. Pendant l'induction de la lésion de glande mammaire (24 h), on a détecté seulement 1,2 % des neutrophiles CD14+ après traitement par le PBS et 1,3 % après traitement par le LPS. La résolution fut caractérisée par une augmentation de la proportion de neutrophiles CD14+. Jusqu'à 96 heures, après injection de PBS, à la différence de celle de LPS, la proportion de ne...

show abstract

“…Using flow cytometry, recruited monocytes and neutrophils could easily be discriminated from rAM in the BAL fluid, because the rAM strongly accumulated the red fluorescent dye PKH26. The gating of cell populations based on FL1 or FSC versus FL2 (PKH26) properties as described by Maus et al (2001) was confirmed by positive staining of monocytes for CD14 and neutrophils for Ly6G/6C and cytospin analysis (data not shown). Figure 6 shows representative dot plots of BAL cells from a vehicle-treated (Fig.…”

Section: Jnj-27141491 Inhibits Recruitment Of Monocytes and Neutrophimentioning

confidence: 75%

Pharmacological Profile of JNJ-27141491 [(S)-3-[3,4-Difluorophenyl)-propyl]-5-isoxazol-5-yl-2-thioxo-2,3-dihydro-1H-imidazole-4-carboxyl Acid Methyl Ester], as a Noncompetitive and Orally Active Antagonist of the Human Chemokine Receptor CCR2

Buntinx¹,

Hermans²,

Goossens³

et al. 2008

J Pharmacol Exp Ther

View full text Add to dashboard Cite

The interaction between CC chemokine receptor 2 (CCR2) with monocyte chemoattractant proteins, such as MCP-1, regulates the activation and recruitment of inflammatory leukocytes. In this study, we characterized (S)-3-[3,4-difluoro-phenyl)-propyl]-5-isoxazol-5-yl-2-thioxo-2,3-dihydro-1H-imidazole-4-carboxyl acid methyl ester (JNJ-27141491) as a noncompetitive and orally active functional antagonist of human (h)CCR2. JNJ-27141491 strongly suppressed hCCR2-mediated in vitro functions, such as MCP-1-induced guanosine 5Ј-O-(3-[35 S]thio)triphosphate binding; MCP-1, -3, and -4-induced Ca 2ϩ mobilization; and leukocyte chemotaxis toward MCP-1 (IC 50 ϭ 7-97 nM), whereas it had little or no effect on the function of other chemokine receptors tested. The inhibition of CCR2 function was both insurmountable and reversible, consistent with a noncompetitive mode of action. JNJ-27141491 blocked the binding of CCR2 is a G protein-coupled chemokine receptor, expressed on monocytes, macrophages, basophils, dendritic cells, natural killer cells, and activated T lymphocytes, that mediates the activation and movement of responsive leukocytes along a chemotactic gradient. CCR2 has two isoforms (CCR2A and CCR2B) that are generated by alternative splicing and differ only in their carboxyl-terminal tail. Leukocytes predominantly express the longer CCR2B variant (Tanaka et al., 2002). CCR2B (henceforth called CCR2) is activated by several members of the CC-chemokine subfamily, consisting of monocyte chemoattractant protein-1 (MCP-1/CCL2), MCP-2/CCL8, MCP-3/CCL7, MCP-4/CCL13, and MCP-5/CCL16. MCP-1 exclusively interacts with CCR2 and is therefore recognized as the prime CCR2 agonist. Engagement of CCR2 inhibits adenylyl cyclase, promotes intracellular calcium mobilization, stimulates mitogen-activated protein kinase and phosphatidylinositol 3-kinase pathways, and promotes che-

show abstract

Monocytes recruited into the alveolar air space of mice show a monocytic phenotype but upregulate CD14

Cited by 125 publications

References 29 publications

Acute lung injury after allogeneic stem cell transplantation: is the lung a target of acute graft-versus-host disease?

Acute lung injury after allogeneic stem cell transplantation: is the lung a target of acute graft-versus-host disease?

Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Pharmacological Profile of JNJ-27141491 [(S)-3-[3,4-Difluorophenyl)-propyl]-5-isoxazol-5-yl-2-thioxo-2,3-dihydro-1H-imidazole-4-carboxyl Acid Methyl Ester], as a Noncompetitive and Orally Active Antagonist of the Human Chemokine Receptor CCR2

Contact Info

Product

Resources

About