Activation of phagocytes during initiation
and resolution of mammary gland injury
induced by lipopolysaccharide in heifers

Sládek, Z.; Rysanek, D.; Faldyna, Martin

doi:10.1051/vetres:2002007

Cited by 29 publications

(37 citation statements)

References 20 publications

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“…PBS-treated mammary glands were set as a control for the MDP and LPS, as was done in our previous studies (Sladek and Rysanek, 2000;Sladek et al, 2002).…”

Section: Induction Of Inflammatory Responsementioning

confidence: 99%

“…Lipopolysaccharide (LPS), a component of Gram-negative bacteria's cellular wall, and muramyldipeptide (MDP), as a minimal structural unit of peptidoglycan in Gram-positive bacteria, have an ability to induce inflammatory response (Sladek and Rysanek, 2000;Rysanek et al, 2001;Sladek et al, 2002).…”

mentioning

confidence: 99%

“…The role of the membrane receptor CD14 in the heifer mammary gland was studied in mastitis induced by LPS, S. aureus and S. uberis. It was found that the inflammatory response of the mammary gland to LPS was associated with an expression of CD14 receptors by PMN and macrophages (Paape et al, 1996;Sladek et al, 2002), whereas expression of CD14 receptors greatly depends on the stage of the inflammatory response within the mammary gland. In the initial phase, a decreased expression of CD14 (Paape et al, 1996) was observed, while there was an increasing expression of resolution characteristics in the inflammatory process (Sladek et al, 2002).…”

mentioning

confidence: 99%

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Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide

Langrova¹,

Sládek²,

Rysanek³

2008

Vet. Med. - Czech

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The aim of the study was to prove the effect of muramyldipeptide and lipopolysaccharide on the expression of CD14 and CD44 during an induced inflammatory response of the mammary gland and its resolution. The purpose was to clarify whether the CD14 and CD44 expression is controlled by the mechanisms of resolution. The CD44 had previously been judged to be an activation marker along with CD11b on polymorphonuclear leukocytes. The experimental inflammatory response was induced by muramyldipeptide and lipopolysaccharide, while phosphate buffered saline was used as a control. The course of the inflammatory response was monitored at four time points: 24 h and 48 h (initiation of inflammatory response), 72 h and 168 h (resolution of inflammatory response). The total number of cells was determined by a hemocytometer. Flow cytometry was used to determine differential counts of leukocytes, proportions of CD11b+ polymorphonuclear leukocytes, proportions of apoptotic and necrotic polymorphonuclear leukocytes, and proportions of CD14+ and CD44+ polymorphonuclear leukocytes. The proportion of CD11b+ polymorphonuclear leukocytes after induction of inflammation with muramyldipeptide was higher (P < 0.05) compared to that after induction by phosphate buffered saline, was highly significantly greater after lipopolysaccharide (P < 0.01), and remained at approximately the same level for the whole period of observation (168 h). A higher proportion of CD14+ polymorphonuclear leukocytes was observed 72 h after induction with phosphate buffered saline. A statistically highly significant lower proportion was observed after induction with muramyldipeptide (P < 0.01), and a statistically significant lower proportion was observed after induction with lipopolysaccharide (P < 0.05). Decrease in the proportion of CD14+ polymorphonuclear leukocytes followed. In the initial phase of the inflammatory response (24 to 72 h) there was a gradual increase in the proportion of CD44+ polymorphonuclear leukocytes, and more so after the phosphate buffered saline. A greatly lower proportion of CD44+ polymorphonuclear leukocytes was observed after administration of muramyldipeptide and lipopolysaccharide: 24 h (P < 0.01), 48 h (P < 0.05) and 72 h (P < 0.01). Compared with muramyldipeptide and lipopolysaccharide, there was a statistically highly significant (P < 0.01) lower proportion of CD44+ polymorphonuclear leukocytes observed 168 h after induction with phosphate buffered saline. Hence the proportion of CD44+ polymorphonuclear leukocytes is low in the initial phase of inflammation, and CD44, in contrast with CD11b, does not appear to be a polymorphonuclear marker of activation. The results of the study have shown that expression of CD14 and CD44 is controlled by the factors inducing inflammatory response as well as by the mechanisms of resolution.

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“…PBS-treated mammary glands were set as a control for the MDP and LPS, as was done in our previous studies (Sladek and Rysanek, 2000;Sladek et al, 2002).…”

Section: Induction Of Inflammatory Responsementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide

Langrova¹,

Sládek²,

Rysanek³

2008

Vet. Med. - Czech

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“…Percentages of individual cell types -granulocytes, monocytes, macrophages and lymphocytes -were read from forward-scatter versus side-scatter dot plots as described previously (Sladek et al 2002) (Figure 1).…”

Section: Animalsmentioning

confidence: 99%

“…Therefore, CD14 serves as a key receptor and recently it has been the subject of intensive research in the bovine mammary gland. Differential expression of CD14 has been observed in freshly migrated inflammatory macrophages (Paape et al 1996;Sladek et al 2002), in resident macrophages from alveoli (Leitner et al 2003;Sladek and Rysanek 2006) and from surrounding tissue (Leitner et al 2003;Dallard et al 2009), and finally in blood monocytes (Paape et al 1996). In blood monocytes, the expression of CD14 is strongly associated with cell viability.…”

mentioning

confidence: 99%

Sladek Z, Rysanek D: CD14 expression, apoptosis and necrosis in resident and inflammatory macrophages from virgin bovine mammary gland

Sládek¹,

Rysanek²

2014

Vet. Med. - Czech

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This paper investigates the association between expression of CD14 and occurrence of apoptosis in blood monocytes, resident ( RES MAC) and inflammatory macrophages from heifer mammary glands after infusion of PBS ( INF MAC PBS ) or LPS ( INF MAC LPS ). Resident macrophages ( RES MAC) were obtained before, and inflammatory macrophages ( INF MAC PBS and INF MAC LPS ) 24 h after, induction of an inflammatory response using phosphate buffered saline (PBS) and lipopolysaccharide (LPS) in mammary glands of unbred heifers. Cell samples were analysed for differential counts, CD14 expression, apoptosis and necrosis using flow cytometry. In vitro cultivation led to a decrease in the proportion of living cells and to an increase in the proportion of apoptotic and necrotic cells in all macrophages and blood monocytes. In CD14 + macrophages, the proportions of live cells increased and proportions of apoptotic and necrotic cells decreased after in vitro cultivation. We observed in CD14 − macrophages and monocytes that the proportions of live cells decreased and proportions of apoptotic and necrotic cells increased after in vitro cultivation. Our experiments confirm that the expression of CD14 in bovine mammary gland macrophages and blood monocytes is associated with cell viability.

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Bovine neutrophils in health and disease

Bassel

Caswell

2018

Cell Tissue Res

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Bovine neutrophils have similarities to those of other species with respect to mechanisms of their activation and migration into tissue, modulation of immune responses and the balance between microbial killing and host tissue damage. However, bovine neutrophils have biochemical and functional differences from those of other species, which may yield insights about the comparative biology of neutrophils. Neutrophils play protective and harmful roles in the infectious diseases of cattle that occur at times of transition: respiratory disease in beef calves recently arrived to feedlots and mastitis and other diseases of postparturient dairy cows. An important research focus is the mechanisms by which risk factors for these diseases affect neutrophil function and thereby lead to disease and the prospect of genetic or pharmacologic improvement of disease resistance. Further, in keeping with the One Health paradigm, cattle can be considered a model for studying the role of neutrophils in naturally occurring diseases caused by host-adapted pathogens and are thus an intermediary between studies of mouse models and investigations of human disease. Finally, the study of bovine neutrophils is important for agriculture, to understand the pathogenesis of these production-limiting diseases and to develop novel methods of disease prevention that improve animal health and reduce the reliance on antimicrobial use.

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Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide in heifers

Cited by 29 publications

References 20 publications

Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide

Expression of CD14 and CD44 on bovine polymorphonuclear leukocytes during resolution of mammary inflammatory response induced by muramyldipeptide and lipopolysaccharide

Sladek Z, Rysanek D: CD14 expression, apoptosis and necrosis in resident and inflammatory macrophages from virgin bovine mammary gland

Bovine neutrophils in health and disease

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