Monocytes in multiple sclerosis: phenotype and cytokine profile

Kouwenhoven, Mathilde C.M.; Teleshova, Natalia; Özenci, Volkan; Press, Rayomand; Link, Hans

doi:10.1016/s0165-5728(00)00396-9

Cited by 85 publications

(59 citation statements)

References 38 publications

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“…The ratio between the percentages of T cells producing IFN-␥ (a Th type-1 cytokine) vs IL-13 and vs IL-4 (Th type-2 cytokines) is higher after priming with DCs from SP-MS compared with those that were primed with DCs from RR-MS and from healthy controls. severity and duration of MS (20). CD80 can indirectly induce secretion of cytokines such as IL-12 and TNF-␣ via induction of CD40L expression on T cells, which in turn will further activate the DCs to produce these cytokines (21).…”

Section: Discussionmentioning

confidence: 99%

Innate Immunity in Multiple Sclerosis: Myeloid Dendritic Cells in Secondary Progressive Multiple Sclerosis Are Activated and Drive a Proinflammatory Immune Response

Karni

Abraham

Monsonego

et al. 2006

The Journal of Immunology

156

118

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Multiple sclerosis (MS) is postulated to be a T cell-mediated autoimmune disease characterized clinically by a relapsing-remitting (RR) stage followed by a secondary progressive (SP) phase. The progressive phase is felt to be secondary to neuronal degenerative changes triggered by inflammation. The status of the innate immune system and its relationship to the stages of MS is not well understood. Dendritic cells (DCs) are professional APCs that are central cells of the innate immune system and have the unique capacity to induce primary immune responses. We investigated circulating myeloid DCs isolated directly from the blood to determine whether there were abnormalities in myeloid DCs in MS and whether they were related to disease stage. We found that SP-MS subjects had an increased percentage of DCs expressing CD80, a decreased percentage expressing PD-L1, and an increased percentage producing IL-12 and TNF-α compared with RR-MS or controls. A higher percentage of DCs from both RR and SP-MS patients expressed CD40 compared with controls. We then investigated the polarization effect of DCs from MS patients on naive T cells taken from cord blood using a MLR assay. Whereas DCs from RR-MS induced higher levels of Th1 (IFN-γ, TNF-α) and Th2 (IL-4, IL-13) cytokines compared with controls, DCs from SP-MS only induced a polarized Th1 response. These results demonstrate abnormalities of DCs in MS and may explain the immunologic basis for the different stages and clinical patterns of MS.

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Section: Discussionmentioning

confidence: 99%

Innate Immunity in Multiple Sclerosis: Myeloid Dendritic Cells in Secondary Progressive Multiple Sclerosis Are Activated and Drive a Proinflammatory Immune Response

Karni

Abraham

Monsonego

et al. 2006

The Journal of Immunology

156

118

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“…Another possible explanation for the induced radioresistance observed in MS lymphocytes could be related to the production of the cytokine Interleukin-12 by the monocytes or macrophages, in the progression of multiple sclerosis (Kouwenhoven et al 2001). It has been shown that IL-12 inhibits the Fas mediated lymphocyte apoptosis and that increased levels of IL-12 are associated with the relapsing phase of the disease.…”

Section: Chromosomal Radiosensitivity Of Lymphocytes From Multiple Scmentioning

confidence: 99%

Chromosomal Radiosensitivity in Secondary‐Progressive Multiple Sclerosis Patients

Petcu

Savu

Vral³

et al. 2004

International Journal of Radiation Biology

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“…Percoll (Pharmacia, Uppsala, Sweden) density gradient separation as previously described [26] with modifications was used to separate monocytes from blood. In brief, 20×10 6 MNC were collected and centrifuged at 500×g for 10 min at 4°C.…”

Section: Preparation Of Monocytesmentioning

confidence: 99%

Multiple sclerosis: elevated expression of matrix metalloproteinases in blood monocytes

Kouwenhoven

Özenci

Gomes

et al. 2001

Journal of Autoimmunity

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Monocytes in multiple sclerosis: phenotype and cytokine profile

Cited by 85 publications

References 38 publications

Innate Immunity in Multiple Sclerosis: Myeloid Dendritic Cells in Secondary Progressive Multiple Sclerosis Are Activated and Drive a Proinflammatory Immune Response

Innate Immunity in Multiple Sclerosis: Myeloid Dendritic Cells in Secondary Progressive Multiple Sclerosis Are Activated and Drive a Proinflammatory Immune Response

Chromosomal Radiosensitivity in Secondary‐Progressive Multiple Sclerosis Patients

Multiple sclerosis: elevated expression of matrix metalloproteinases in blood monocytes

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