Monocytes in Icelandic horses: comparison between automatic gating by the ADVIA 2120i, a manual gating method and microscopic differentiation

Leidinger, E.; Prechtl, G.; Figl, Julia; Leidinger, Judith

doi:10.1007/s00580-015-2080-5

Comp Clin Pathol

2015

DOI: 10.1007/s00580-015-2080-5

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Monocytes in Icelandic horses: comparison between automatic gating by the ADVIA 2120i, a manual gating method and microscopic differentiation

E. Leidinger¹,

G. Prechtl

Julia Figl³

et al.

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Cited by 2 publications

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“…Finally, Run Screen can convert RBC, Baso, Perox, and Retics raw data to a le format (*.fcs) readable by ow cytometry-dedicated software (WinMDI; Purdue University Cytometry Laboratories, West Lafayette, IN, USA). Only two-dimensional images are displayed in Run Screen, but single-parameter, dual-parameter, and three-dimensional histograms can be observed using WinMDI [5].…”

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Analysis of Raw Data Produced by Advia 2120i Using Virtual Machine Technology in Remote Offices

et al. 2021

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Analysis of Raw Data Produced by Advia 2120i Using Virtual Machine Technology in Remote Offices

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Application of the ASVCP guidelines for the establishment of haematologic and biochemical reference intervals in Icelandic horses in Austria

Leidinger¹,

Leidinger²,

Figl³

et al. 2015

Acta Vet Scand

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BackgroundDespite the increasing popularity of Icelandic horses, published reference intervals (RIs) in this breed are rare. Due to their isolation and their small gene pool, alterations in some variables are likely and some possible breed-specific peculiarities have been described. The purpose of the present study was the establishment of comprehensive RIs in Icelandic horses according to recently published guidelines.In a prospective observational study, blood samples were collected from the jugular vein of 142 Icelandic horses into EDTA and serum tubes. Reference intervals were established for haematologic and biochemical analytes on the Advia 2120i™ and the Dimension ExL™ by established methods. RIs were defined as central 95 % intervals bounded by the 2.5th and 97.5th percentiles with their 90 % confidence intervals, calculated according to recently published ASVCP guidelines. An inhouse-developed quality control system using observed total allowable error was used for the surveillance of the internal quality control preceding the measurements.ResultsThe RIs were as follows: haematocrit: 0.29–0.39, RBC: 5.79–8.63 T/l, haemoglobin: 102.0–142.3 g/l, MCV: 42–51 fl, platelets: 146–263 G/l, WBC: 4.13–8.57 G/l, segs: 1.98–4.73 G/l, lymphocytes: 1.25–3.49 G/l, monocytes: 0.06–0.31 G/l, eosinophils: 0.04–0.50 G/l, glucose: 4.0–5.7 mmol/l, urea: 3.2–6.4 mmol/l, creatinine: 79.6–141.4 μmol/l, total protein: 54.4–72.9 g/l, albumin: 27.7–36.8 g/l, total bilirubin: 8.1–21.1 μmol/l, triglycerides: 0.03–0.44 mmol/l, cholesterol: 1.75–2.90 mmol/l, ALP: 1.35–3.55 μkat/l, AST: 4.52–8.80 μkat/l, GLDH: 0.0–0.18 μkat/l, GGT: 0.11–0.39 μkat/l, CK: 2.53–6.52 μkat/l, LDH: 3.32–7.95 μkat/l, iron: 16.4–39.9 μmol/l, calcium: 2.69–3.19 mmol/l, phosphate: 0.5–1.3 mmol/l, magnesium: 0.6–0.9 mmol/l, sodium: 134–141 mmol/l, potassium: 3.6–4.7 mmol/l, chloride: 100–105 mmol/l.ConclusionsReference intervals of several haematologic and biochemical analytes differed from the transferred historical reference intervals applied to equine samples in the authors’ laboratory. These might be of clinical importance in some analytes such as creatine kinase.Electronic supplementary materialThe online version of this article (doi:10.1186/s13028-015-0120-4) contains supplementary material, which is available to authorized users.

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Monocytes in Icelandic horses: comparison between automatic gating by the ADVIA 2120i, a manual gating method and microscopic differentiation

Cited by 2 publications

References 7 publications

Analysis of Raw Data Produced by Advia 2120i Using Virtual Machine Technology in Remote Offices

Analysis of Raw Data Produced by Advia 2120i Using Virtual Machine Technology in Remote Offices

Application of the ASVCP guidelines for the establishment of haematologic and biochemical reference intervals in Icelandic horses in Austria

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