Monocyte phagocytosis as a reliable parameter for predicting early-onset sepsis in very low birthweight infants

Hallwirth, Ulrike; Pomberger, Gerhard; Zaknun, Daniela; Szépfalusi, Zsolt; Horcher, Ernst; Pollak, Arnold; Roth, Erich; Spittler, Andreas

doi:10.1016/s0378-3782(01)00245-6

Cited by 38 publications

(29 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We found decreased HLA-DR expression on monocytes from preterm neonates (20). Recently, we have demonstrated that monocytes from VLBWI display decreased phagocyte activity in newborns developing septic complications (6). It has also been shown that the expression of the CD14 antigen on mature monocytes, a cell surface receptor involved in the response to LPS, is dependent on gestational maturation (19).…”

Section: Discussionmentioning

confidence: 64%

See 1 more Smart Citation

Monocyte Toll-Like Receptor 4 Expression and LPS-Induced Cytokine Production Increase during Gestational Aging

et al. 2005

Self Cite

View full text Add to dashboard Cite

Premature newborns are highly susceptible to severe bacterial infections. This is partially due to their immature innate immune system, characterized by decreased neutrophil and monocyte activity as well as by reduced concentrations of complement factors. However, additional mechanisms might be important for innate immunity and are still the subject of considerable debate. The importance of pattern recognition domains such as Toll-like receptors (TLR) has been fully acknowledged within the last few years. Therefore, we investigated age-related monocyte TLR4 expression and lipopolysaccharide-induced cytokine secretion from very low birth weight infants (VLBWI) and from newborns after wk 30 of gestation in comparison to healthy adults. In VLBWI, expression of TLR4 surface protein, detected by flow cytometry, and TLR4-specific mRNA, quantified by real time-PCR, were significantly reduced in comparison to mature infants and to adults. Reduced TLR4 expression was paralleled by significantly diminished ex vivo LPS stimulated IL-1␤, IL-6, and tumor necrosis factor-␣ secretion into whole blood. We conclude that, in VLBWI, the minimized expression of TLR4 contributes to the susceptibility of VLBWI to infections with Gram-negative bacteria due to the lack of cytokines to boost initial immune response. (Pediatr Res 58: 121-124, 2005) Abbreviations CB, cord blood LPS, lipopolysaccharide PAMP, pathogen-associated molecular pattern PB, peripheral blood PBMC, peripheral blood mononuclear cells TLR, Toll-like receptor TNF, tumor necrosis factor VLBWI, very low birth weight infants Bacterial infections are a major cause of neonatal morbidity (1,2), and premature newborns are highly susceptible to infections (3). This increased susceptibility appears to be related to immaturity of the immune system. Maturation of the immune system includes specific humoral and cellular responses as well as nonspecific innate immune mechanisms, such as factors of the complement system and phagocyte activity (3-5).Monocytes and macrophages play a central role in both specific and nonspecific immunity against bacterial, viral, and fungal infection. In VLBWI, a substantial decrease of fully functional phagocytes has recently been reported (6). The Toll protein has been identified as a receptor controlling dorsoventral polarization during embryonic development of Drosophila larvae and serves a defensive function (7). Structures homologous to Toll receptors were found in mammalian cells (8,9) and termed TLR. Within the last few years, 10 human TLR have been described and characterized as important sensors of the innate immune system (9,10). These TLR are phylogenetically conserved and are involved in recognition of microbial PAMP. TLR4 functions as the transmembrane component of the LPS receptor. In addition to LPS, the major glycolipid component of the outer membrane of Gram-negative bacteria, TLR4 recognizes saturated and unsaturated fatty acids such as lipoteichoic acids from Gram-positive bacteria, and heat-shock proteins (8 -10). Binding of these ...

show abstract

Section: Discussionmentioning

confidence: 64%

“…In VLBWI, a substantial decrease of fully functional phagocytes has recently been reported (6). The Toll protein has been identified as a receptor controlling dorsoventral polarization during embryonic development of Drosophila larvae and serves a defensive function (7).…”

mentioning

confidence: 99%

Monocyte Toll-Like Receptor 4 Expression and LPS-Induced Cytokine Production Increase during Gestational Aging

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, nutritional supplementation of weanling pigs with cholecalciferol or 25(OH)D significantly increased the phagocytic capacity of blood monocytes and granulocytes (Konowalchuk et al 2013). The percentages of phagocytic monocytes and PMNLs in the whole blood of lambs at birth were similar to those seen in the cord blood (Hallwirth et al 2002) and peripheral blood (Prosser et al 2013) of human infants, and, as expected, the ability of the lamb monocytes and PMNLs to engulf the bacterial bioparticles was higher at 4 weeks of age than at birth, reflecting maturation of the lamb's immune system (Menge et al 1998). …”

Section: Discussionmentioning

confidence: 69%

Supplementation of Merino ewes with cholecalciferol in late pregnancy improves the vitamin D status of ewes and lambs at birth but is not correlated with an improvement in immune function in lambs

et al. 2016

View full text Add to dashboard Cite

Functional deficiencies of the immune system are known to predispose human and animal neonates to death. Thus, immune competency may be a significant factor influencing the mortality of lambs.Vitamin D has been recognised to improve immune function and is transferred across the placenta.This study tested the hypotheses that (1) supplementation of Merino ewes with cholecalciferol during late pregnancy will increase the concentrations of vitamin D in the ewe and lamb at birth and (2) supplementation of Merino ewes with cholecalciferol during late pregnancy is correlated with an increase in innate phagocytic and adaptive antibody immune responses in the lamb. Merino ewes Supplementation with cholecalciferol had no significant effect on the phagocytic capacity of monocytes or polymorphonuclear leukocytes, the concentration of IgG in the colostrum or plasma of lambs, or the vaccine-specific antibody response against tetanus toxoid. Overall, the results support our first hypothesis, but suggest that maternal supplementation with 1 × 10 6 IU cholecalciferol does not improve innate, passive or adaptive immune function in lambs.

show abstract

“…However, the correlation between phagocytosis in cord and peripheral blood has not been reported previously. Phagocytosis of EC by monocytes from cord blood and day 3 peripheral blood has been investigated but no statistical comparisons were reported (28). Our data are therefore important in verifying cord blood as a suitable and convenient surrogate to explore early peripheral responses in infants.…”

Section: Phagocytosis In Newborn Whole Bloodmentioning

confidence: 94%

Phagocytosis of neonatal pathogens by peripheral blood neutrophils and monocytes from newborn preterm and term infants

et al. 2013

View full text Add to dashboard Cite

Monocyte phagocytosis as a reliable parameter for predicting early-onset sepsis in very low birthweight infants

Cited by 38 publications

References 32 publications

Monocyte Toll-Like Receptor 4 Expression and LPS-Induced Cytokine Production Increase during Gestational Aging

Monocyte Toll-Like Receptor 4 Expression and LPS-Induced Cytokine Production Increase during Gestational Aging

Supplementation of Merino ewes with cholecalciferol in late pregnancy improves the vitamin D status of ewes and lambs at birth but is not correlated with an improvement in immune function in lambs

Phagocytosis of neonatal pathogens by peripheral blood neutrophils and monocytes from newborn preterm and term infants

Contact Info

Product

Resources

About