Phagocytosis of neonatal pathogens by peripheral blood neutrophils and monocytes from newborn preterm and term infants

Prosser, Amy; Hibbert, Julie; Strunk, Tobias; Kok, Chooi Heen; Simmer, Karen; Richmond, Peter; Burgner, David; Currie, Andrew

doi:10.1038/pr.2013.145

Cited by 48 publications

(41 citation statements)

References 44 publications

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“…In this first investigation into woylie immune function, we adapted a phagocytosis flow cytometry protocol developed in very pre-term human infants [35] to characterise factors that affect phagocytosis in the woylie. Using this protocol, uniquely combined with minimally invasive physiology and parasitology techniques in individually-identified woylies sampled repeatedly over time, we determined that host stress physiology and Trypanosoma infection status influence phagocytosis.…”

Section: Discussionmentioning

confidence: 99%

“…We investigated phagocytosis in woylie by adapting a flow cytometry protocol developed for very pre-term human infants [35]. As this was the first time that flow cytometry has been carried out to assess phagocytosis in the woylie, the amount of fluorescently labelled bacteria added to whole blood was first titrated.…”

Section: Phagocytosis Flow Cytometry Assaymentioning

confidence: 99%

“…As this was the first time that flow cytometry has been carried out to assess phagocytosis in the woylie, the amount of fluorescently labelled bacteria added to whole blood was first titrated. Titration was performed by adding phRodo® Red Samples were prepared for flow cytometry as described by Prosser et al (2013) with some modifications [35]. In brief, as per the woylie titration, 10ul of phRodo® was added.…”

Section: Phagocytosis Flow Cytometry Assaymentioning

confidence: 99%

“…The ex vivo nature of the phagocytosis flow cytometry assay also has advantages over in vivo immunologic tests such as allograft rejection, which may be ethically challenging to carry out in critically endangered species. The phagocytosis flow cytometry protocol adapted for this study [35] was particularly well suited for use in the woylie as it requires small blood volumes (<25μl) and, with some modifications, did not require species-specific markers or reagents.…”

Section: Introductionmentioning

confidence: 99%

“…We adapted a small blood volume phagocytosis flow cytometry protocol developed for very preterm human infants [35] and validated in newborn lambs [26], to perform the first study of immune function of the woylie. We then investigated factors, which influence host immunity and host-parasite interactions to determine their impact, if any, on phagocytosis.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Host stress physiology and Trypanosoma haemoparasite infection influence innate immunity in the woylie ( Bettongia penicillata )

Hing

Currie

Broomfield

et al. 2016

Comparative Immunology, Microbiology and Infectious Diseases

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Understanding immune function is critical to conserving wildlife in view of infectious disease threats, particularly in threatened species vulnerable to stress, immunocompromise and infection. However, few studies examine stress, immune function and infection in wildlife.We used a flow cytometry protocol developed for human infants to assess phagocytosis, a key component of innate immunity, in a critically endangered marsupial, the woylie (Bettongia penicillata). The effects of stress physiology and Trypanosoma infection on phagocytosis were investigated. Blood and faecal samples were collected from woylies in a captive facility over three months. Trypanosoma status was determined using PCR.

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Section: Discussionmentioning

confidence: 99%

Section: Phagocytosis Flow Cytometry Assaymentioning

confidence: 99%

Section: Phagocytosis Flow Cytometry Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Host stress physiology and Trypanosoma haemoparasite infection influence innate immunity in the woylie ( Bettongia penicillata )

Hing

Currie

Broomfield

et al. 2016

Comparative Immunology, Microbiology and Infectious Diseases

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Bacterial biofilm formation and immune evasion mechanisms

Snowden¹

2016

The Human Microbiota and Chronic Disease

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Impact of pregravid obesity on maternal and fetal immunity: Fertile grounds for reprogramming

Sureshchandra

Marshall

Messaoudi

2019

Journal of Leukocyte Biology

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Maternal pregravid obesity results in several adverse health outcomes during pregnancy, including increased risk of gestational diabetes, preeclampsia, placental abruption, and complications at delivery. Additionally, pregravid obesity and in utero exposure to high fat diet have been shown to have detrimental effects on fetal programming, predisposing the offspring to adverse cardiometabolic, endocrine, and neurodevelopmental outcomes. More recently, a deeper appreciation for the modulation of offspring immunity and infectious disease‐related outcomes by maternal pregravid obesity has emerged. This review will describe currently available animal models for studying the impact of maternal pregravid obesity on fetal immunity and review the data from clinical and animal model studies. We also examine the burden of pregravid obesity on the maternal–fetal interface and the link between placental and systemic inflammation. Finally, we discuss future studies needed to identify key mechanistic underpinnings that link maternal inflammatory changes and fetal cellular reprogramming events.

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Phagocytosis of neonatal pathogens by peripheral blood neutrophils and monocytes from newborn preterm and term infants

Cited by 48 publications

References 44 publications

Host stress physiology and Trypanosoma haemoparasite infection influence innate immunity in the woylie ( Bettongia penicillata )

Host stress physiology and Trypanosoma haemoparasite infection influence innate immunity in the woylie ( Bettongia penicillata )

Bacterial biofilm formation and immune evasion mechanisms

Impact of pregravid obesity on maternal and fetal immunity: Fertile grounds for reprogramming

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