Monocyte/macrophage cytokine activity regulates vascular smooth muscle cell function within a degradable polyurethane scaffold

Battiston, Kyle G.; Ouyang, Ben; Labow, Rosalind S.; Simmons, Craig A.; Santerre, J. Paul

doi:10.1016/j.actbio.2013.12.022

Cited by 37 publications

(31 citation statements)

References 64 publications

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“…cellular attraction, migration, proliferation, and differentiation) and eventual ECM deposition and remodeling. 5,[12][13][14] Which factors are secreted is determined by the macrophage phenotypical state, which in turn depends on the phase of tissue repair and the cellular microenvironment. 6,7 Herein, a rough division can be made between the pro-inflammatory M1 phenotype, important for host-defense, and the anti-inflammatory M2 phenotypes (e.g.…”

Section: Introductionmentioning

confidence: 99%

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

et al. 2020

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“…Both conditions led to a better infiltration and contractile phenotype than SMCs alone. A direct coculture method and SMC culture in a monocyte‐conditioned medium yielded similar results regarding growth and infiltration …”

Section: Tissue‐specific Coculture Processesmentioning

confidence: 76%

“…In order to achieve consistent cell attachment and migration, monocytes were used as an alternative cell source to ECs in coculture on porous PLGA composite scaffolds . Using monocytes led to beneficial effects from their secreted chemokines, cytokines and growth factors on SMC attachment, proliferation and migration . With the hypothesis that the preceding release of cytokines and chemokines from monocytes would enhance SMC infiltration in the pores prior to cell–cell contact, a combination of monocytes and SMCs in immediate (contact at t = 0) and delayed (addition of the second cell types at t = 24 h) coculture assays on polyurethane films was assessed .…”

Section: Tissue‐specific Coculture Processesmentioning

confidence: 99%

Multilineage Constructs for Scaffold‐Based Tissue Engineering: A Review of Tissue‐Specific Challenges

Baudequin

Tabrizian

2017

Adv Healthcare Materials

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There is a growing interest in the regeneration of tissue in interfacial regions, where biological, physical, and chemical attributes vary across tissue type. The simultaneous use of distinct cell lineages can help in developing in vitro structures, analogous to native composite tissues. This literature review gathers the recent reports that have investigated multiple cell types of various sources and lineages in a coculture system for tissue-engineered constructs. Such studies aim at mimicking the native organization of tissues and their interfaces, and/or to improve the development of complex tissue substitutes. This paper thus distinguishes itself from those focusing on technical aspects of coculturing for a single specific tissue. The first part of this review is dedicated to variables of cocultured tissue engineering such as scaffold, cells, and in vitro culture environment. Next, tissue-specific coculture methods and approaches are covered for the most studied tissues. Finally, cross-analysis is performed to highlight emerging trends in coculture principles and to discuss how tissue-specific challenges can inspire new approaches for regeneration of different interfaces to improve the outcomes of various tissue engineering strategies.

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“…14 Polyurethane scaffolds have been used for bone, skin, soft tissue, vascular, and drug delivery applications, although few studies investigate cartilage engineering applications. 17,18,[40][41][42][43][44] Our previous experience and success with the current biodegradable polyurethane matrix in developing products for burn wound management led to this study of its application in cartilage repair and engineering. We have established that the biodegradable polyurethane matrix serves as a suitable scaffold for tissue engineering cartilage using chondrocytes with and without perichondrocytes as a cell source.…”

Section: Discussionmentioning

confidence: 97%

A Novel Biodegradable Polyurethane Matrix for Auricular Cartilage Repair

Iyer

Dearman

Wagstaff

et al. 2016

Journal of Burn Care & Research

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Auricular reconstruction poses a challenge for reconstructive and burns surgeons. Techniques involving cartilage tissue engineering have shown potential in recent years. A biodegradable polyurethane matrix developed for dermal reconstruction offers an alternative to autologous, allogeneic, or xenogeneic biologicals for cartilage reconstruction. This study assesses such a polyurethane matrix for this indication in vivo and in vitro. To evaluate intrinsic cartilage repair, three pigs underwent auricular surgery to create excisional cartilage ± perichondrial defects, measuring 2 × 3 cm in each ear, into which acellular polyurethane matrices were implanted. Biopsies were taken at day 28 for histological assessment. Porcine chondrocytes ± perichondrocytes were cultured and seeded in vitro onto 1 × 1 cm polyurethane scaffolds. The total culture period was 42 days; confocal, histological, and immunohistochemical analyses of scaffold cultures were performed on days 14, 28, and 42. In vivo, the polyurethane matrices integrated with granulation tissue filling all biopsy samples. Minimal neocartilage invasion was observed marginally on some samples. Tissue composition was identical between ears whether perichondrium was left intact, or not. In vitro, the polyurethane matrix was biocompatible with chondrocytes ± perichondrocytes and supported production of extracellular matrix and Type II collagen. No difference was observed between chondrocyte culture alone and chondrocyte/perichondrocyte scaffold coculture. The polyurethane matrix successfully integrated into the auricular defect and was a suitable scaffold in vitro for cartilage tissue engineering, demonstrating its potential application in auricular reconstruction.

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Monocyte/macrophage cytokine activity regulates vascular smooth muscle cell function within a degradable polyurethane scaffold

Cited by 37 publications

References 64 publications

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

Hemodynamic loads distinctively impact the secretory profile of biomaterial-activated macrophages – implications forin situvascular tissue engineering

Multilineage Constructs for Scaffold‐Based Tissue Engineering: A Review of Tissue‐Specific Challenges

A Novel Biodegradable Polyurethane Matrix for Auricular Cartilage Repair

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