Monoclonal platelet antigen capture assays (MAIPA) and reagents: a statement

Kaplan, C.; Freedman, John; Foxcroft, Zyta Krystyna; Husebekk, Anne; Metcalfe, Paul; Muñiz‐Díaz, Eduardo; Ouwehand, Willem H.; Panzer, Simon; Rožman, Primož; Skogen, Bjøŕn

doi:10.1111/j.1423-0410.2007.00943.x

Cited by 25 publications

(21 citation statements)

References 13 publications

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“…In selected cases, MACE is used to detect HPA antibodies reactive with HPA‐2a/b. A slightly different approach, designated monoclonal antibody immobilization of platelet antigens (MAIPA) is widely used in Europe (Kaplan et al , ). MACE and MAIPA are probably equivalent in sensitivity and specificity.…”

Section: Laboratory Diagnosis Of Naitmentioning

confidence: 99%

Neonatal alloimmune thrombocytopenia: pathogenesis, diagnosis and management

et al. 2013

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Summary Neonatal alloimmune thrombocytopenia, (NAIT) is caused by maternal antibodies raised against alloantigens carried on fetal platelets. Although many cases are mild, NAIT is a significant cause of morbidity and mortality in newborns and is the most common cause of intracranial haemorrhage in full-term infants. In this report, we review the pathogenesis, clinical presentation, laboratory diagnosis and prenatal and post-natal management of NAIT and highlight areas of controversy that deserve the attention of clinical and laboratory investigators.

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Section: Laboratory Diagnosis Of Naitmentioning

confidence: 99%

Neonatal alloimmune thrombocytopenia: pathogenesis, diagnosis and management

et al. 2013

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“…This prevents a reduction in sensitivity because of the loss of clinically important epitopes and reduces the incidence of false‐positives because of the formation of clinically irrelevant neo‐epitopes. To date, the MAIPA is considered to be the gold standard reference technique in platelet immunology [13]. The ability to detect maternal alloantibodies varied widely between laboratories [14] and drawbacks of the technique must not be ignored.…”

Section: Laboratory Testingmentioning

confidence: 99%

Advances in alloimmune thrombocytopenia laboratory investigations

Kaplan¹

2009

ISBT Science Series

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Alloimmune thrombocytopenia is mainly encountered during pregnancy when the mother becomes immunized against the fetal platelets antigens she lacks. The resulting fetal alloimmune thrombocytopenia, which incidence has been evaluated to 1/1000 live births in Caucasians, is usually severe and can lead to bleeding. Intracranial hemorrhage (20‐30% in retrospective studies) is the most severe complication leading to death (10‐15%) or neurological sequelae (20%). Due to the recurrence for incompatible fetuses in the subsequent pregnancies, antenatal management has been developed. The diagnosis of fetal or neonatal alloimmune thrombocytopenia is of utmost importance for the management of the affected infant and the subsequent pregnancies. The diagnosis is straightforward when a maternal alloantibody is detected directed against the offending antigen present in the infant. Although genotyping is widely used, unknown genetic variants may alter the results, and ethnic diversity is of importance. Genotype is not always phenotype. However phenotyping is somehow problematic because reference human sera have limited availability. The detection of the alloantibodies could be challenging. Currently the most widely techniques used are the antigen‐capture assays with mouse monoclonal antibodies. However false‐negative results may occur. New techniques are developed. In conclusion, international workshop exercises are of importance for further improvement and quality assurance proficiency.

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“…Since the MAIPA assay is highly sensitive and specific, it is considered as the gold standard technique in platelet immunology [26]. The preparation of a wellcharacterized panel of platelets is necessary for the determination of HPA antibodies.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a cell line panel for the detection of antibodies against human platelet antigen 4b

Hayashi

Amakishi²,

Inoue

et al. 2011

Int J Hematol

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Antibodies against human platelet antigens (HPAs) play important roles in thrombocytopenia. In Japan, HPA-4b antibody is frequently responsible for HPA-related neonatal alloimmune thrombocytopenia. A highly sensitive assay using platelets has been developed for the detection of antibodies against HPAs. However, it is difficult to obtain the platelets expressing specific HPAs required for the assay. Therefore, an alternative method not requiring platelets would be helpful to detect antibodies against HPAs. Glycoprotein IIIa (GPIIIa) cDNA encoding HPA-4b was individually co-transduced with that of wild-type GPIIb in K562 cells, which is a non-adherent cell line, using a retroviral vector. The expression of transgene products in cultured cells were observed for over 6 months. Next, to evaluating the sensitivity and specificity of this cell line panel, we performed monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay with a serum previously identified by another method. All HPA-4b antibodies in serum samples were positive, and all serum samples, including normal serum and serum containing HLA antibodies were negative. No difference was observed in the specificity and sensitivity between our method and conventional MAIPA using platelets. The present results indicate that this established cell line panel permits highly sensitive detection of specific antibodies against HPA-4b.

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Monoclonal platelet antigen capture assays (MAIPA) and reagents: a statement

Cited by 25 publications

References 13 publications

Neonatal alloimmune thrombocytopenia: pathogenesis, diagnosis and management

Neonatal alloimmune thrombocytopenia: pathogenesis, diagnosis and management

Advances in alloimmune thrombocytopenia laboratory investigations

Establishment of a cell line panel for the detection of antibodies against human platelet antigen 4b

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