Monoclonal IgMs with anti-Gal(β1–3)GalNAc activity in lower motor neuron disease; identification of glycoprotein antigens in neural tissue and cross-reactivity with serum immunoglobulins

Thomas, Florian P.; Lee, Ann M.; Romas, Stavra N.; Latov, N.

doi:10.1016/0165-5728(89)90036-2

Cited by 49 publications

(13 citation statements)

References 31 publications

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“…However, the use of antibodies alone to identify glycosphingolipids, gangliosides in particular, should be done with caution, because these compounds share similar sugar moieties with many other molecules present on the cell membrane (i.e., glycoproteins, glycosaminoglycans, phosphatidyl glycosides, etc.) and this could cause cross-reactivity (molecular mimicry), hence ambiguous results (11)(12)(13)(14)(15)(16)(17). Actually, molecular mimicry may also occur in the case of carbohydrate mimicking peptides ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, in the more general routine, glycosphingolipids have been identifi ed only by the use of immunometric procedures employing specifi c anti-individual glycosphingolipid antibodies, quite useful for cytochemical investigations. Nonetheless, the specifi city of anti-glycosphingolipid (particularly gangliosides) antibodies may often be poor, due to cross-reactivity (molecular mimicry) with other glycoconjugates, glycoproteins, glycosaminoglycans, and phosphatidyl glycosides (11)(12)(13)(14)(15)(16)(17). Still, as some glycosphingolipids, including gangliosides GM2, GD2, and GD3, are unequivocally expressed on the cell surface of several tumors (i.e., neuroblastoma, melanoma, and sarcoma), the possibility of specifi cally targeting them for an immunotherapeutic strategy has led to an increasing interest in developing new, and more specifi c, anti-ganglioside antibodies ( 18 ).…”

Section: Rna Extraction and Real-time Pcrmentioning

confidence: 99%

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Gangliosides as a potential new class of stem cell markers: the case of GD1a in human bone marrow mesenchymal stem cells

Bergante¹,

Torretta²,

Creo³

et al. 2014

Journal of Lipid Research

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Section: Discussionmentioning

confidence: 99%

Section: Rna Extraction and Real-time Pcrmentioning

confidence: 99%

Gangliosides as a potential new class of stem cell markers: the case of GD1a in human bone marrow mesenchymal stem cells

Bergante¹,

Torretta²,

Creo³

et al. 2014

Journal of Lipid Research

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“…The present study is the first that showed an occurrence of neutral glycolipid containing asialoGM 1 (Cheer) epitope in the PNS and its myelins, although its presence in CNS myelin was reported previously (Kusunoki et al, 1985). Its terminal disaccharide is considered to be Gal@1-3GalNAc, which is shared by GMl ganglioside and also by some glycoproteins found in PNS myelin (Shuman et al, 1983;Thomas et al, 1989). The glycolipid containing GhCer epitope in our study appears likely to be GhCer itself, judging from its behaviors in TLC analyses, although its chemical structure remains to be determined.…”

Section: Discussionmentioning

confidence: 47%

Glycosphingolipids of Human Peripheral Nervous System Myelins Isolated from Cauda Equina

Ogawa-Goto

Ohta

Kubota

et al. 1993

Journal of Neurochemistry

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Compositions of neutral and sulfated glucuronyl glycosphingolipids purified from human motor and sensory nerves and myelins were studied. Higher neutral glycosphingolipids (fraction B), which were separated from GalCer (fraction A), were analyzed by TLC and TLC-immunostaining. Both nerve myelins contained paragloboside (nLc4Cer) and nLc6Cer dominantly as major higher glycosphingolipids and very little globoside (Gb4Cer), whereas both nerves contained Gb4Cer and nLc4Cer. Besides these major glycosphingolipids, a neutral glycolipid containing asialoGM1 (Gg4Cer) epitope and other minor components such as ceramide trihexoside and ceramide dihexoside were detected in both nerves and their myelins. Furthermore, sulfated glucuronyl nLc4Cer and nLc6Cer, which were monoclonal antibody HNK-1 reactive glycolipids, were detected in both nerves and myelins.

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“…Regarding GBS, antibodies against some ganglioside glycolipids, in peripheral nerves, and a galactocerebroside, a major glycolipid myelin antigen, have been reported after M. pneumoniae infection [4,8]. Common to all these glycolipids is the galactosyl-(1-3)-N-acetylgalactosaminyl moiety, the same sugar determinant in erythrocytes, peripheral nerves [5] and other tissues. The auto-immune response to the myelin antigen might cause antibody-induced demyelination.…”

mentioning

confidence: 96%

Mycoplasma pneumoniae antigen detection in Guillain-Barré syndrome

Meseguer¹,

Aparicio

Calvo

et al. 1998

European Journal of Pediatrics

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Sir: The role of Mycoplasma pneumoniae in the pathophysiology of Guillain-BarreÂ syndrome (GBS) is unknown. Direct invasion of CSF by the micro-organism in GBS has been proved both by immuno¯uorescence [1] and culture [3] although an auto-immune mechanism is the most supported theory, with the key role of a demyelinating process.A 3-year-old boy was admitted to our hospital with a 6-day history of progressive weakness in his lower limbs, frequent falls and diculty in walking. Previously, he had mild respiratory tract symptoms. The most remarkable ®ndings were muscle weakness, absence of tendon re¯exes in the lower limbs and clumsy gait. Haemoglobin was 8.7 g/dl, haematocrit 25.8%, the ESR 25 mm/h and he had positive IgM anticardiolipin antibodies. Cold agglutinins were negative. CSF (lumbar puncture) revealed 1 leucocyte/ mm 3 , protein, 72 mg/dl, glucose, 54 mg/dl, IgG, 7.4 mg/dl (952 mg/dl in serum), and albumin, 44 mg/dl (3,920 mg/dl in serum). The CSF specimen was inadequate to culture for M. pneumoniae. Serological investigations for respiratory viruses (adenovirus, in¯uenza A, and B, para-in¯uenza 1, 2, and 3, cytomegalovirus, respiratory syncytial), and Epstein Barr viruses as well as stool culture for Campylobacter jejuni were negative. Based on conduction velocities in both right and left peroneal nerves of 24 and 17.9 m/s respectively, (normal range: 41±64 m/s), decreased amplitude of the compound muscle action potentials, and absence of sensory sural nerve responses, a diagnosis of GBS was made.Two serum specimens (days 5 and 21) revealed positive IgM and a four-fold increase in IgG antibodies (ELISA Virotech, Germany, and ELISA Bio Whittaker, Walkersville, USA, respectively) to M. pneumoniae. M. pneumoniae antigen was directly detected by a monoclonal antibody ELISA (Pneumofast Ag, International Microbio. Signes, France), in a throat swab and aspirated respiratory secretions.Intravenous immunoglobulins were administered (0.4 g/kg per day for 5 days) with no clinical improvement. A 10-day oral clarythromycin (15 mg/kg per day) course followed, with rapid improvement 2 days after initiating therapy. Tendon re¯exes became normal on day 21.

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Monoclonal IgMs with anti-Gal(β1–3)GalNAc activity in lower motor neuron disease; identification of glycoprotein antigens in neural tissue and cross-reactivity with serum immunoglobulins

Cited by 49 publications

References 31 publications

Gangliosides as a potential new class of stem cell markers: the case of GD1a in human bone marrow mesenchymal stem cells

Gangliosides as a potential new class of stem cell markers: the case of GD1a in human bone marrow mesenchymal stem cells

Glycosphingolipids of Human Peripheral Nervous System Myelins Isolated from Cauda Equina

Mycoplasma pneumoniae antigen detection in Guillain-Barré syndrome

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