Monoclonal antibody-based immunoassay of vitellogenin in the blood of male channel catfish (Ictalurus punctatus)

Goodwin, Andrew E.; Grizzle, John M.; Bradley, James T.; Estridge, Barbara H.

doi:10.1016/0305-0491(92)90025-m

Cited by 45 publications

(44 citation statements)

References 17 publications

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“…Male fish of several species have been reported to have VTG in extreme low concentration even if not exposed to EDCs. 21,22) However those reports were not examined on effect of VTG production when fed to a fish diet containing phytoestrogens and other estrogenic substances, and there is a variety not only of the fish diets but also of the normal physiological VTG concentration before an experiment for EDCs. Therefore in the development of the screening test for estrogenic compounds based on VTG induction in fish, it was necessary to exceed the normal physiological VTG concentration in male and female fish when fed to diet containing phytoestrogens and other estrogenic substances.…”

Section: Discussionmentioning

confidence: 99%

Induction of Plasma Vitellogenin Synthesis by the Commercial Fish Diets in Male Goldfish(Carassius auratus) and Dietary Phytoestrogens.

Ishibashi

Kobayashi

Koshiishi

et al. 2002

JOURNAL OF HEALTH SCIENCE

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In this study, we analyzed contents of phytoestrogens (genistein, daidzein, equol, and coumestrol) in two commercial fish diets [a diet for trout (TD) and a diet for ornamental carp (CD)] using Liquid Chromatography-Mass Spectroscopy/Mass Spectroscopy (LC-MS/MS), and these contents were compared with that of a casein-based formulated fish diet (FD) which does not contain soya bean or fish meal. We also analyzed phytoestrogen contents in commercial infant casein-and soya bean-based diets. The contents of phytoestorogens were generally high in CD, TD, and soya milk, and low or non-detectable in FD and casein-based milks. Among these samples, CD showed the highest phytoestrogen contents: genistein, 390800 ng/g; daidzein, 416800 ng/g; coumestrol, 1325 ng/g; equol, 6.4 ng/ g. We also determined the estrogenic activity of the fish diets using male goldfish by measuring plasma vitellogenin (VTG) levels as a biomarker of estrogen exposure. When male goldfish were fed one of these diets for 31 days, plasma VTG was detected in CD-fed fish (78.01 ± 48.18 µg/ml) and TD-fed fish (3.51 ± 3.83 µg/ml), whereas plasma VTG was not detected in FD-fed fish (less than 0.040 µg/ml). These results indicate that the commercial fish diets examined contain a large amount of phytoestrogens and showed estrogenic activity that were strong enough to induce VTG production in male goldfish. It is necessary to eliminate estrogenic substances other than test chemicals in the screening test system for estrogenic endocrine-disrupting chemicals (EDCs). Since the formulated diet developed in the present study contain less phytoestrogens than the commercial fish diets and has low estrogenic activity, it is suggested that VTG production using male goldfish in combination with the low estrogen fish diet is a good in vivo system for evaluation of estrogenic effects of EDCs.

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Section: Discussionmentioning

confidence: 99%

Induction of Plasma Vitellogenin Synthesis by the Commercial Fish Diets in Male Goldfish(Carassius auratus) and Dietary Phytoestrogens.

Ishibashi

Kobayashi

Koshiishi

et al. 2002

JOURNAL OF HEALTH SCIENCE

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“…However, since males normally have no vitellogenin, the expression of any vitellogenin serves as an ideal biomarker for xenobiotic estrogenic stimulation. Vitellogenin expression has been used successfully for identification of exposure to environmental estrogens in fish, including wild populations (26,27), and under laboratory (28) and in vitro conditions (29,30).…”

Section: Introductionmentioning

confidence: 99%

Vitellogenin Induction by Xenobiotic Estrogens in the Red-Eared Turtle and African Clawed Frog

Palmer¹,

Palmer²

1995

Environmental Health Perspectives

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Many environmental pollutants have estrogenic activity in animals. Xenobiotic estrogens include many pesticides and industrial chemicals that bioaccumulate. The impact of these common pollutants on the reproductive success of wildlife may be considerable, particularly in threatened or endangered species. This research examined the use of plasma vitellogenin in males as a biomarker for estrogenic xenobiotics in reptiles and amphibians. Adult male turtles (Trachemys scripta) and frogs (Xenopus laevis) were given ip injections of estradiol-1 7f (E2), diethylstilbestrol (DES), or o,p'DDT (1-chloro-2-[2,2,2-trichloro-1-(4-chlorophenyl)ethyllbenzene) daily for 7 days, and plasma was collected on day 14. The estrogenic activity of each compound was determined by measuring the induction of plasma vitellogenin. Vitellogenin was identified by precipitation, electrophoresis, Western blot, and enzyme-linked immunosorbant assay (ELISA). In both species, estradiol and DES treatments induced the most vitellogenin, whereas DDT treatments induced smaller amounts of vitellogenin in a dose-dependent fashion. These data indicate that induction of plasma vitellogenin in males may be a useful biomarker of xenobiotic estrogen activity in wild populations of reptiles and amphibians. -Environ Health Perspect 103 (Suppl 4):19-25 (1995)

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“…Homologous radioimmunoassays have been developed for Atlantic salmon, rainbow trout, brown trout, coho salmon, carp, and eels (So et al 1985;Sumpter 1985;Norberg and Haux 1988;Benfey et al 1989;Tyler and Sumpter 1990;BurzawaGerard and Dumas-Vidal 1991). Recently enzymelinked immunosorbent assays (ELISAs) have been developed for vitellogenin from sole (Nunez Rodriguez et al 1989), striped bass (Kishida et al 1992), and channel catfish (Goodwin et al 1992). However, no immunoassay has been developed for vitellogenin in tilapia.…”

Section: Introductionmentioning

confidence: 99%

Vitellogenin in tilapia (Oreochromis mossambicus): Induction of two forms by estradiol, quantification in plasma and characterization in oocyte extract

Kishida

Specker

1993

Fish Physiol Biochem

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Two forms of vitellogenin were isolated by DEAE agarose ion-exchange chromatography from plasma of the tilapia, Oreochromis mossambicus. The monomers have apparent molecular masses of 200 and 130 kDa, as indicated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and a total amount of phosphorus of 1.7 and 0.1%, respectively. Antibodies specific to the two forms, designated tVTG-200 and tVTG-130, were generated in rabbits and used to develop enzyme-linked immunosorbent assays (ELISAs) and in Western blot analyses of plasma and oocyte extract. SDS-PAGE of the oocyte extract showed a major protein band at 106.6, minor bands at 26.6, 24.2, and 23.7 kDa, and very faint bands at 83.4 and 17.5 kDa. Western blots of the oocyte extract revealed that the antiserum to tVTG-200 recognized strongly the protein bands at 24.2 and 23.7 kDa, and less strongly the bands at 25.1 and 22.6 kDa, whereas the antiserum to tVTG-130 recognized mainly the protein band at 106.6 kDa. The presence of both VTGs in untreated male tilapia was detected with the ELISAs using relatively high plasma volumes. Their presence in males was confirmed by VTG-like immunoreactive materials eluting from the ion-exchange column at the same positions as tVTG-200 and tVTG-130. The concentrations of the VTGs in males were several orders of magnitude lower than in vitellogenic females. Treatment of male tilapia with estradiol-17β (E2) induced both VTGs within 24h. After 7 days, tVTG-130 reached a maximum concentration in plasma, whereas tVTG-200 continued to increase. Our findings demonstrate that the two vitellogenins are biochemically distinct, possibly differentially regulated, and made by both sexes.

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Monoclonal antibody-based immunoassay of vitellogenin in the blood of male channel catfish (Ictalurus punctatus)

Cited by 45 publications

References 17 publications

Induction of Plasma Vitellogenin Synthesis by the Commercial Fish Diets in Male Goldfish(Carassius auratus) and Dietary Phytoestrogens.

Induction of Plasma Vitellogenin Synthesis by the Commercial Fish Diets in Male Goldfish(Carassius auratus) and Dietary Phytoestrogens.

Vitellogenin Induction by Xenobiotic Estrogens in the Red-Eared Turtle and African Clawed Frog

Vitellogenin in tilapia (Oreochromis mossambicus): Induction of two forms by estradiol, quantification in plasma and characterization in oocyte extract

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