Monoclonal antibody 3F8 recognises the neural cell adhesion molecule (NCAM) in addition to the ganglioside GD2

Patel, Kalpana; Rossell, RJ; Pemberton, LF; Nk, Cheung; Walsh, F. S.; Moore, SE; Sugimoto, T.; Kemshead, J T

doi:10.1038/bjc.1989.380

Cited by 18 publications

(11 citation statements)

References 41 publications

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“…It was suggested that one such Mab provided a well-needed addition to immunomarkers capable of distinguishing SCLC from non-SCLC, in agreement with our opinion that polyclonal anti-NCAM antibodies could be a useful diagnostic tool. Similarly, the potential therapeutic use of polyclonal anti-NCAM antibodies suggested here, is supported by a recent report [39] describing the use of a Mab, directed against the carbohydrate epitope common to NCAM and the ganglioside GD2 for immunotherapy and targeted radiotherapy. Another of these studies [41] on the SCLC H69 cell line showed the presence of two NCAM isoforms of 180 and 140 kDa and also high levels of polysialic acid, which was suggested to be implicated in the invasive behaviour of these tumors.…”

Section: Resultssupporting

confidence: 72%

NCAM: a surface marker for human small cell lung cancer cells

et al. 1990

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Immunocytochemicaland immunochemical techniques were used to study the expression of the neural cell adhesion molecule (NCAM) by human lung cancer cell lines. Intense surface staining for NCAM was found at light and electron microscopic levels on small cell lung cancer cells. The NCAM polypeptide of M, 140000 (NCAM 140) was detected by immunoblotting in all of 7 small cell lung cancer cell lines examined and in one out of two of the closely related large cell cancer cell lines: it was not detected in cell lines obtained from one patient with a mesothelioma, in two cases of adenocarcinoma, nor in two cases of squamous cell cancer. In contrast, neuron-specific enolase was found by immunoblotting in all the lung cancer cell lines tested and synaptophysin in all but the adenocarcinoma cell lines. These antigens were localized intracellularly. The specific expression of NCAM 140 by human small and large cell lung carcinomas suggests its potential as a diagnostic marker.Lung cancer; Neural cell adhesion molecule; Marker

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Section: Resultssupporting

confidence: 72%

NCAM: a surface marker for human small cell lung cancer cells

et al. 1990

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“…Recently, the presence of 140-kDa NCAM on ES cell lines was confirmed by FCI with 6H7 and Leu-19 antibodies [24]. A study with Mab 3F8, raised following immunization of mice with neuroblastoma cells and recognizing sialic acid residues epitope of NCAM, showed indirect immunofluorescence reactivity of all four studied cell lines of ES [18]. On the other hand, immunohistochemical studies have generally failed to show NCAM expression in ES tissue.…”

Section: Discussionmentioning

confidence: 98%

Identification of CD56 and CD57 by flow cytometry in Ewing's sarcoma or primitive neuroectodermal tumor

et al. 1998

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CD56 and CD57 are commonly considered as natural killer and neuroectodermal markers, but their expression has been identified in a wide spectrum of neoplasms including some cases of Ewing's sarcoma (ES) and primitive neuroectodermal tumor (PNET). We report two cases of small, round blue cell tumor (SRBCT), in which flow cytometry immunophenotyping (FCI) detected strong expression of CD56 and CD57 (one case). Immunohistochemical staining with Leu-19 and Leu-7 confirmed the FI results. Although CD56 and CD57 expression is consistent with ES/PNET, it can be potentially misleading if results of FCI are interpreted in the absence of other findings. These cases suggest the utility of FCI in undifferentiated SRBCT. The literature on CD56 and CD57 expression in ES/PNET is reviewed and discussed.

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“…The observed unexpectedly high affinity of mAb735 for long PSAs was also reflected in the need for rigorous regeneration after binding of MenB polysaccharide in the SPR, as well as in the difficulties in the removal of bound mAb735 from immunoblots of polysialylated NCAM. 1 As some gangliosides have been suggested to cross-react with oligosialic acid antibodies (Patel et al, 1989), the reactivity of their oligosaccharide-analogues, mono-and disialosyl lactose was tested. Although high concentrations (up to 10 mM) of disialyllactose, sialyllactose and lactose were used in the assay, no binding could be demonstrated.…”

Section: Specificity Of the Interactionmentioning

confidence: 99%

High affinity binding of long-chain polysialic acid to antibody, and modulation by divalent cations and polyamines

Häyrinen¹,

Haseley²,

Talaga³

et al. 2002

Molecular Immunology

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Long-chain polysialic acid (PSA) is expressed on the vertebrate neural cell adhesion molecule (NCAM) during neuronal plasticity. Its structural similarity to the capsular PSAs of some pathogenic bacteria has hampered the development of polysaccharide vaccines against meningitis. The antibodies formed during immunization require a long epitope for binding, and cross-react with host tissue PSA. The nature of the epitope and possible external effectors involved are still unclear. We have evaluated the interaction of PSA with its antibody mAb735 by surface plasmon resonance. The influences of PSA chain length, pH, temperature, ionic environment, and polyamines were also determined.The antibody binding affinity was found to dramatically increase with PSA chain length. A sub-nanomolar dissociation constant (K D = 8.5 × 10 −10 M) was obtained for the binding of very long chain native MenB polysaccharides (∼200 Neu5Ac-residues). Colominic acid from Escherichia coli K1 (∼100 residues) and shorter polymers exhibited progressively weaker affinities. The antibody also bound tightly (K D ∼ 5 × 10 −9 M) to polysialylated glycopeptides from human embryonal brain. The effects of pH and ionic strength suggested that the interaction is largely electrostatic. Ca 2+ and Mn 2+ ions promoted the observed surface plasmon resonance response in a concentration dependent fashion. Spermine increased the response in a similar way. Our results suggest that divalent cations and polyamines may play significant role in the regulation of the PSA epitope presentation in vivo.

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Monoclonal antibody 3F8 recognises the neural cell adhesion molecule (NCAM) in addition to the ganglioside GD2

Cited by 18 publications

References 41 publications

NCAM: a surface marker for human small cell lung cancer cells

NCAM: a surface marker for human small cell lung cancer cells

Identification of CD56 and CD57 by flow cytometry in Ewing's sarcoma or primitive neuroectodermal tumor

High affinity binding of long-chain polysialic acid to antibody, and modulation by divalent cations and polyamines

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