Monoclonal antibodies with specificity for hairy cell leukemia cells.

Genetic control of T cell receptor BJ gene expression in peripheral lymphocytes of normal and rheumatoid arthritis monozygotic twins.

“…The genetically determined TCRBJ biases were well displayed by CD4 ϩ T cells but poorly by CD8 ϩ T cells. Clonal expansions in peripheral CD8 ϩ T cells have been reported both in healthy individuals and in autoimmune disease patients, but have not been attributed to a specific environmental stimulus (26)(27)(28)(29)(30)(31)(32)(33). In this regard, the expanded transcripts in the RA patients were not limited to specific BV-BJ combinations.…”

Rearrangement and expression of immunoglobulin genes and expression of Tac antigen in hairy cell leukemia.

“…However, no fully equivalent cell within normal cellular differentiation has been identified. Several investigations have found the presence of B-cell-associated surface antigens (5)(6)(7), whereas others have reported T-cell-associated surface antigens (8)(9)(10) upon these leukemic cells. The vast majority of cases do bear surface Ig, but the presence of avid Fc fragment receptors (11) and reports of multiple Ig isotypes (5,12) have raised questions as to whether the Ig in all cases was actually synthesized by the neoplastic cells.…”

Scanning Immunoelectron Microscopy of Hairy Cell Leukemia

“…It appears, however, that accurate diagnosis of HCL may be made with a battery of monoclonal antibodies when some precautions are taken to avoid nonspecificity. In our study, hairy cell-'specific' monoclonal anti bodies (HC-1, HC-2) [ 17] were tested but proved to be useless for immuno-SEM since they did not recognize the antigen after glutaraldehyde prefixation. This lat ter was necessary for satisfactory cell surface preser vation, and to avoid capping and endocytosis of the antigen or clumping of the gold particles.…”

“…The previously characterized monoclonal antibodies utilized in this study were: B1 (Coulter Electronics) which recognizes all ma ture B cells [18]; BAI (Hybritech) which reacts with mantle zone B cells [I]; OKMI (Ortho Diagnostics) which recognizes an antigen present on mature monocytes and granulocytes [3]; anti-TAC, which reacts with an antigen of activated T cells (kindly provided by Dr. R. Veda, Nagoya, Japan [22]); LeuM5 (Becton-Dickinson), which recognizes an antigen present on all hairy cells and on mono cytes and macrophages [21 ]; FMC17 which recognizes mature mon ocytes [4] (a gift of Dr. H. Zola, Bedford Park, Australia): HC-I and HC-2 which react with some hairy cells, some leukemic myelomonoblasts and some activated B cells [17] (kindly provided by Dr. D.JV. Posnett, New York).…”