Monoclonal Antibodies to the Enterotoxins and to the Toxic Shock Syndrome Toxin Produced by Staphylococcus aureus

Thompson, Nancy E.; Bergdoll, Merlin S.; Meyer, Richard F.; Bennett, Reginald W.; Miller, L.; Macmillan, James D.

doi:10.1016/b978-0-12-463002-4.50009-4

Cited by 14 publications

(15 citation statements)

References 58 publications

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“…Positive controls proved the successful binding of the toxins to the microtiter plates. Hitherto, MAbs had been produced after immunization of mice with SEC 2 or SEC 3 and therefore they reacted with other toxins of the SEC group (Thompson et al 1984(Thompson et al , 1985. The only anti-SEC l mAb produced so far (Thompson et al 1984) reacted also with SEB.…”

Section: Discussionmentioning

confidence: 94%

“…None of the MAbs produced reacted with SEA, SEC2, SEC3, SED, and SEE inspite of the strong serological relationships within the SEC group (Reiser et al 1984; Thompson et al 1985). Positive controls proved the successful binding of the toxins to the microtiter plates.…”

Section: Discussionmentioning

confidence: 95%

“…The immunization of the seven-week-old BALB/c mice with SEB or SEC 1 emulsified in Freund's complete adjuvant was performed according to Thompson et al (1985). For each toxin a total of six mice were immunized.…”

Section: Immunizationmentioning

confidence: 99%

“…The number of monoclonal antibodies reacting with SEB is about the same as the number which react with SEC1, SEC2, SEC 3 but may be lower with the other staphylococcal enterotoxins. All of the monoclonal antibodies developed so far against the SEC group react with all or at least with two subtypes of SEC or even with SEB (Edwin et al 1986;Meyer et al 1984;Thompson et al 1984Thompson et al , 1985Thompson et al , 1986. As shown by Thompson et al (1986) the use of MAbs reduces the number of assays needed to detect all of the serological types of enterotoxins.…”

Section: Introductionmentioning

confidence: 97%

“…An important advantage of using the MAb system is the unlimited supply of highly uniform reagents. So far, there are seven serologically distinct staphylococcal enterotoxins (SEA, SEB, SEC1, SEC2, SEC3, SED, and SEE) (Thompson et al 1985) that are similiar in activity, structure and molecular weight (25,000-30,000) (Bergdoll 1983). Crossreacting determinants, however, were observed between SEA and SEE as well as within the SEB-SEC group (Lee et al 1978;Spero et al 1978), with particular frequency within the SEB-SEC group.…”

Section: Introductionmentioning

confidence: 99%

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Specificity, cross-reactivity and competition profile of monoclonal antibodies to staphylococcal enterotoxins B and C1 detected by indirect enzyme-linked immunosorbent assays

Kienle

Buschmann

1989

Med Microbiol Immunol

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A group of monoclonal antibodies to the staphylococcal enterotoxins B and C1 without any cross-reactivity to the other known staphylococcal enterotoxins A, C2, C3, D and E was developed. The monoclonal antibodies were compared in competition ELISA's with regard to affinity and epitope recognition. Three different groups could be classified: Group 1, consisting of the MAbs B/3-4 and B/3-8, recognizes in identical manner staphylococcal enterotoxin B. Also group 2 (MAb B/3-5) recognizes only staphylococcal enterotoxin B, yet group 1 and 2 do not compete with each other in the competition ELISA and therefore own different paratopes. Group 3 (MAb C1/2-3 and C1/4-6) reacts exclusively with staphylococcal enterotoxin C1, not even with staphylococcal enterotoxins C2 and C3.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 95%

Section: Immunizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Specificity, cross-reactivity and competition profile of monoclonal antibodies to staphylococcal enterotoxins B and C1 detected by indirect enzyme-linked immunosorbent assays

Kienle

Buschmann

1989

Med Microbiol Immunol

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Molecular Structures of Bacteria Elucidated by Monoclonal Antibodies with Special Reference to Antigenic Determinants of the Methanogens' Envelopes

Macario

1986

Monoclonal Antibodies Against Bacteria

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Magnetic Immunoassay for Detection of Staphylococcal Toxins in Complex Media

et al. 2012

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Method of highly sensitive registration of magnetic nanoparticles by their nonlinear magnetization is used in a novel sandwich-type immunoassay for detection of staphylococcal toxins in complex media of virtually any volume, with increasing sensitivity at higher sample volume. The signal is read out from the entire volume of a nontransparent 3D fiber structure employed as a solid phase, which provides large reaction surface, quick reagent mixing, as well as antigen immunofiltration directly in the course of the assay. The method has demonstrated near-linear dose-response curves within a wide range of ~3 decades, while detection of staphylococcal enterotoxin A (SEA) and toxic shock syndrome toxin (TSST) in neat milk without sample preparation. The limits of detection (LOD) as low as 4 and 10 pg/mL for TSST and SEA, respectively, were obtained in 2-h format using 30-mL samples. The second, 25-min format, showed the LOD of 0.1 and 0.3 ng/mL for the same toxins in a 150 μL sample. The developed immunoassay can be applied in food safety control, in vitro diagnostics, and veterinary for a variety of research from express tests in the field to highly sensitive laboratory tests.

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Monoclonal Antibodies to the Enterotoxins and to the Toxic Shock Syndrome Toxin Produced by Staphylococcus aureus

Cited by 14 publications

References 58 publications

Specificity, cross-reactivity and competition profile of monoclonal antibodies to staphylococcal enterotoxins B and C1 detected by indirect enzyme-linked immunosorbent assays

Specificity, cross-reactivity and competition profile of monoclonal antibodies to staphylococcal enterotoxins B and C1 detected by indirect enzyme-linked immunosorbent assays

Molecular Structures of Bacteria Elucidated by Monoclonal Antibodies with Special Reference to Antigenic Determinants of the Methanogens' Envelopes

Magnetic Immunoassay for Detection of Staphylococcal Toxins in Complex Media

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