Monoclonal antibodies for dioxin: Antibody characterization and assay development

Stanker, Larry H.; Watkins, Bruce E.; Rogers, Nina Trivedy; Vanderlaan, Martin

doi:10.1016/0300-483x(87)90015-1

Cited by 57 publications

(67 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The assay was performed with a solid-phase RIA configuration. Other immunoassays using monoclonal antibodies were developed in the 1980s by Stanker et al [141,142] that were applied to detect dioxins in soils [143] and for rapid screening of dioxins and furans [144,145,146,147]. These ELISAs had a sensitivity similar to the RIA (an IC 50 value about 200 pg TCDD well -1 ).…”

Section: Immunochemical Techniques For Pcdfs and Pcddsmentioning

confidence: 98%

Immunochemical determination of xenobiotics with endocrine disrupting effects

Estévez-Alberola

Marco

2004

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

This paper is a review with more than 100 references discussing the immunochemical methods reported in the literature for the most important man-made chemicals with suspected endocrine disrupting activity. Details regarding immunizing hapten design, antibody production, and the features (limit of detection, dynamic range, specificity) of the most important immunochemical methods developed (ELISA, FIIA, immunosorbents, immunosensors, etc.) are presented for important environmental pollutants such as bisphenol A, phthalates, alkylphenol polyethoxylates, alkylphenols, polychlorinated biphenyl compounds, and dioxins. Availability of commercial reagents and methods is reported.

show abstract

Section: Immunochemical Techniques For Pcdfs and Pcddsmentioning

confidence: 98%

Immunochemical determination of xenobiotics with endocrine disrupting effects

Estévez-Alberola

Marco

2004

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

show abstract

“…The development of immunoassays for the PCDDs is complicated by their lipophilic properties, required method sensitivity, and the complex nature of environmental samples. Due to these difficulties, only a few studies have been reported in the development of antibodies and ELISA methods for PCDDs with mixed results (Stanker et al, 1987;Sanborn et al, 1998;Sugawara et al, 1998Sugawara et al, , 2002Harrison and Carlson, 1999;Shan et al, 2001;SW846 Method 4025, 2002;Okuyama et al, 2004;Lo et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples

Emon¹,

Chuang²,

Lordo³

et al. 2008

Chemosphere

View full text Add to dashboard Cite

A 96-microwell enzyme-linked immunosorbent assay (ELISA) method was evaluated to determine PCDDs/PCDFs in sediment and soil samples from an EPA Superfund site. Samples were prepared and analyzed by both the ELISA and a gas chromatography/high resolution mass spectrometry (GC/HRMS) method. Comparable method precision, accuracy, and detection level (8 ng kg −1 ) were achieved by the ELISA method with respect to GC/HRMS. However, the extraction and cleanup method developed for the ELISA requires refinement for the soil type that yielded a waxy residue after sample processing. Four types of statistical analyses (Pearson correlation coefficient, paired t-test, nonparametric tests, and McNemar's test of association) were performed to determine whether the two methods produced statistically different results. The logtransformed ELISA-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin values and logtransformed GC/ HRMS-derived TEQ values were significantly correlated (r = 0.79) at the 0.05 level. The median difference in values between ELISA and GC/HRMS was not significant at the 0.05 level. Low false negative and false positive rates (<10%) were observed for the ELISA when compared to the GC/HRMS at 1000 ng TEQ kg −1 . The findings suggest that immunochemical technology could be a complementary monitoring tool for determining concentrations at the 1000 ng TEQ kg −1 action level for contaminated sediment and soil. The ELISA could also be used in an analytical triage approach to screen and rank samples prior to instrumental analysis.

show abstract

“…A number of methods have been established for dioxins and related compounds, since routine monitoring of these compounds is difficult, time consuming and expensive; thus, screening methods that offer improvements in speed, sample throughput and cost are particularly desirable. Therefore, the early immunoassays for environmental contaminants include several dioxin assays [24]. Harrison et al [25] developed immunoassays against dioxin and related compounds which are now commercially available and which are based on the DD3 monoclonal antibody.…”

Section: Immunoassaysmentioning

confidence: 99%

The potential of mechanism-based bioanalytical tools in ecotoxicological exposure and effect assessment

Eggen

Segner

2003

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

The current challenge to ecotoxicology is to develop tools that allow rapid and cost-efficient detection of those environmental chemicals or their combinations that are responsible for sublethal, chronic toxic effects in exposed organisms. Bioanalytical tools may meet these challenges, particularly if they are mechanism-based. Technically, bioanalytical tools allow rapid and cost-efficient analysis of environmental matrices. Mechanism-based, bioanalytical tools, however, do not only indicate that certain chemicals are there, but-and this is the major advantage of mechanism-based bioanalytical tools (MBBTs)-they indicate that chemicals with a specific mode of toxic action or a specific toxic potential are there. In this way MBBTs bridge exposure and effect assessment and help in a faster identification of the causative agent(s). Several principles of MBBTs, including immunoassays, enzyme inhibition assays, receptor assays and gene induction assays are briefly discussed and their application in processes such as bioassay-directed fractionation is illustrated. The focus of this manuscript is the analytical power of MBBTs in exposure and effect assessment. MBBTs have, however, a much broader potential and can support research on other challenges in ecotoxicology such as mixture effects or multiple effects caused by single pollutants or by various stresses simultaneously.

show abstract

Monoclonal antibodies for dioxin: Antibody characterization and assay development

Cited by 57 publications

References 22 publications

Immunochemical determination of xenobiotics with endocrine disrupting effects

Immunochemical determination of xenobiotics with endocrine disrupting effects

An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples

The potential of mechanism-based bioanalytical tools in ecotoxicological exposure and effect assessment

Contact Info

Product

Resources

About