Monoclonal Antibodies Directed against Human Immunodeficiency Virus (HIV) gag Proteins with Specificity for Conserved Epitopes in HIV-1, HIV-2 and Simian Immunodeficiency Virus

Characterization of Monoclonal Antibodies that Distinguish Simian Immunodeficiency Virus Isolates from each Other and from Human Immunodeficiency Virus Types 1 and 2

“…Lowenstine et al, 1986) and infected African green monkeys (Ohta et al, 1988), which crossreacted with the major core protein (p24) of HIV-1. In other studies, M A b s to HIV-2 p24 cross-reacted with HIV-1, SIVMAc, SIVAc~ and SIVsM (Minassian et al, 1988;Niedrig et al, 1988). The SIVMAc M A b s described here are much more selective in their reactivity, especially with respect to HIV-1 or HIV-2.…”

Identification of a Gag Protein Epitope Conserved Among All Four Groups of Primate Immunodeficiency Viruses by Using Monoclonal Antibodies

“…The cross-reactivity of MAbs against p24 from isolates of the HIV-2/SIVma~ group with SIV~m p24 has been reported (Minassian et al, 1988;Komatsu et al, 1990). The cross-reactivity of MAbs against the matrix protein p17 of different HIV and SIV isolates has also been investigated, but only restricted cross-reactivity was demonstrated (Minassian et al, 1988;Niedrig et al, 1988;Komatsu et al, 1990;Sutjipto et al, 1990). In contrast, the anti-pl7 MAbs investigated here show unrestricted cross-reactivity with all four immunodeficiency virus groups mentioned.…”

Multiple Effects of Codon Usage Optimization on Expression and Immunogenicity of DNA Candidate Vaccines Encoding the Human Immunodeficiency Virus Type 1 Gag Protein

“…Cell culture supernatants were collected 2 days after transfection and clarified by centrifugation at 3,000 ϫ g for 10 min. The Gag content was determined by using a p24 capture ELISA as described earlier in detail (26). The p24-specific monoclonal antibodies 11-G-7 and 10-E-7, used in this assay, were kindly provided by Matthias Niedrig (RKI, Berlin, Germany).…”