Characterization of Monoclonal Antibodies that Distinguish Simian Immunodeficiency Virus Isolates from each Other and from Human Immunodeficiency Virus Types 1 and 2

Sutjipto, Suganto; Kodama, Toshiaki; Yee, JoAnn; Gettie, Agegnehu; Jennings, Myra; Desrosiers, Ronald C.; Marx, Preston A.

doi:10.1099/0022-1317-71-1-247

Cited by 21 publications

(15 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We therefore analyzed macrophages infected with SIVmac-2311 and SIVmac-1A39 for the presence of SIV viral antigen. A mouse monoclonal antibody raised against SIV p279ag was used for immunocytochemical analysis of the infected cells (48). Multinucleated giant cells in SlVmac-2311 cultures stained positive with the anti-SIV p27zag antibody (data not shown).…”

Section: Resultsmentioning

confidence: 96%

See 1 more Smart Citation

Identification of viral determinants of macrophage tropism for simian immunodeficiency virus SIVmac

Banapour¹,

Marthas²,

Ramos³

et al. 1991

J Virol

View full text Add to dashboard Cite

Simian immunodeficiency virus (SIV), a lymphocytopathic lentivirus, induces an AIDS-like disease in rhesus macaques (Macaca mulatta). A pathogenic molecular clone of rhesus macaque SIV (SIVmac), SIVmac-239, replicates and induces cytopathology in T lymphocytes but is restricted for replication in macrophages. In contrast, a nonpathogenic molecular clone of SIVmac, SIVmac-1A11, replicates and induces syncytia (multinucleated giant cells) in cultures of both T lymphocytes and macrophages. SIVmac-1A11 does not cause disease in macaques. To map the viral determinants of macrophage tropism, reciprocal recombinant genomes were constructed between molecular clones of SIVmac-239 and SIVmac-lA1l. Infectious recombinant viruses were rescued by transfection of cloned viral genomes into permissive lymphoid cells. Analysis of one pair of reciprocal recombinants revealed that an internal 6.2-kb DNA fragment of SIVmac-lA11 was necessary and sufficient for both syncytium formation and efficient replication in macrophages. This region includes the coding sequences for a portion of the gag gene, all of the pol, vif, vpr, and vpx genes, the first coding exons of tat and rev, and the external env glycoprotein gpl30. Thus, the transmembrane glycoprotein of env, the nef gene, the second coding exons of tat and rev, and the long terminal repeats are not essential for in vitro macrophage tropism. Analysis of additional recombinants revealed that syncytium formation, but not virus production, was controlled by a 1.4-kb viral DNA fragment in SIVmac-1A11 encoding only the external env glycoprotein gpl30. Thus, gpl30 env of SIVmac-1A11 is necessary for entry of virus into macrophages but is not sufficient for a complete viral replication cycle in this cell type. We therefore conclude that gpl30 env and one or more genetic elements (exclusive of the long terminal repeats, transmembrane glycoprotein of env, and second coding exons of tat and rev, and nefj) are essential for a complete replication cycle of SlVmac in rhesus macaque macrophages.

show abstract

Section: Resultsmentioning

confidence: 96%

“…activity (20). Immunocytochemical analysis of macrophages with a monoclonal antibody to SIVmac p279ag was performed as previously described (1,48).…”

mentioning

confidence: 99%

Identification of viral determinants of macrophage tropism for simian immunodeficiency virus SIVmac

Banapour¹,

Marthas²,

Ramos³

et al. 1991

J Virol

View full text Add to dashboard Cite

show abstract

“…The cross-reactivity of MAbs against p24 from isolates of the HIV-2/SIVma~ group with SIV~m p24 has been reported (Minassian et al, 1988;Komatsu et al, 1990). The cross-reactivity of MAbs against the matrix protein p17 of different HIV and SIV isolates has also been investigated, but only restricted cross-reactivity was demonstrated (Minassian et al, 1988;Niedrig et al, 1988;Komatsu et al, 1990;Sutjipto et al, 1990). In contrast, the anti-pl7 MAbs investigated here show unrestricted cross-reactivity with all four immunodeficiency virus groups mentioned.…”

Section: Abteilung Virologie Und Immunologie Deutsches Primatenzentmentioning

confidence: 85%

Identification of a Gag Protein Epitope Conserved Among All Four Groups of Primate Immunodeficiency Viruses by Using Monoclonal Antibodies

Otteken

Nick²,

Bergter

et al. 1992

Journal of General Virology

View full text Add to dashboard Cite

“…Heat-induced epitope retrieval was performed with DIVA Decloaker and then blocked with SNIPER Reagent (Biocare, Walnut Creek, CA) for 15 min. The sections were incubated with a SIVgag monoclonal antibody (clone FA2, AIDS Reagent Repository Program)(16) and rabbit anti CD4 and CD8 polyclonal antibodies (Abcam, Cambridge, MA) for 1h, followed by Mach2 Double-stain detection kit (Biocare medical) for 30 min. Antibodies were detected by development of the chromogens (Vulcan Fast Red and Vina Green chromogen kit; Biocare medical).…”

Section: Methodsmentioning

confidence: 99%

Spatial Alterations between CD4+ T Follicular Helper, B, and CD8+ T Cells during Simian Immunodeficiency Virus Infection: T/B Cell Homeostasis, Activation, and Potential Mechanism for Viral Escape

Hong¹,

Amancha²,

Rogers³

et al. 2012

The Journal of Immunology

143

194

View full text Add to dashboard Cite

HIV/SIV infections induce chronic immune activation with remodeling of lymphoid architecture and hypergammaglobulinemia, although the mechanisms leading to such symptoms remain to be fully elucidated. Moreover, lymph nodes have been highlighted as a predilection site for SIV escape in vivo. Following 20 rhesus macaques infected with SIVmac239, as they progress from pre to acute and chronic infection, we document for the first time the local dynamics T follicular helper (TFH) cells and B cells in situ. Progression of SIV infection was accompanied with increased numbers of well delineated follicles containing germinal centers (GCs) and TFH cells with a progressive increase in the density of PD-1 expression in lymph nodes. The rise in PD-1+ TFH cells was followed by a substantial accumulation of Ki67+ B cells within GCs. However, unlike in blood, major increases in the frequency of CD27+ memory B cells were observed in lymph nodes, indicating increased turnover of these cells, correlated with increases in total and SIV specific antibody levels. Of importance, compared to T cell zones, GCs seemed to exclude CD8+ T cells while harboring increasing numbers of CD4+ T cells, many of which are positive for SIVgag, providing an environment particularly beneficial for virus replication and reservoirs. Our data highlight for the first time important spatial interactions of GC cell subsets during SIV infection, the capacity of lymphoid tissues to maintain stable relative levels of circulating B cell subsets and a potential mechanism for viral reservoirs within GCs during SIV infection.

show abstract

Characterization of Monoclonal Antibodies that Distinguish Simian Immunodeficiency Virus Isolates from each Other and from Human Immunodeficiency Virus Types 1 and 2

Cited by 21 publications

References 16 publications

Identification of viral determinants of macrophage tropism for simian immunodeficiency virus SIVmac

Identification of viral determinants of macrophage tropism for simian immunodeficiency virus SIVmac

Identification of a Gag Protein Epitope Conserved Among All Four Groups of Primate Immunodeficiency Viruses by Using Monoclonal Antibodies

Spatial Alterations between CD4+ T Follicular Helper, B, and CD8+ T Cells during Simian Immunodeficiency Virus Infection: T/B Cell Homeostasis, Activation, and Potential Mechanism for Viral Escape

Contact Info

Product

Resources

About