Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection

Abstract

Lateral flow assays (lateral flow immunoassays and nucleic acid lateral flow assays) have experienced a great boom in a wide variety of early diagnostic and screening applications. As opposed to conventional examinations (High Performance Liquid Chromatography, Polymerase Chain Reaction, Gas chromatography-Mass Spectrometry, etc.), they obtain the results of a sample’s analysis within a short period. In resource-limited areas, these tests must be simple, reliable, and inexpensive. In this review, we outline the production process of antibodies against drugs of abuse (such as heroin, amphetamine, benzodiazepines, cannabis, etc.), used in lateral flow immunoassays as revelation or detection molecules, with a focus on the components, the principles, the formats, and the mechanisms of reaction of these assays. Further, we report the monoclonal antibody advantages over the polyclonal ones used against drugs of abuse. The perspective on aptamer use for lateral flow assay development was also discussed as a possible alternative to antibodies in view of improving the limit of detection, sensitivity, and specificity of lateral flow assays.

Interpol Review of Drug Analysis 2019-2022

“…2021 review of the production process of antibodies against drugs of abuse used in lateral flow immunoassays as detection molecules, with a focus on the components, the principles, the formats, and the mechanisms of reaction of these assays as well as the advantages of monoclonal antibodies over the polyclonal [ 1028 ].…”

Advances on Bioanalysis: Recent Approaches in the Determination of Biomarkers, Drugs of Abuse and Medicines

“…Finally, lateral flow immunoassays and nucleic acid lateral flow assays in a wide variety of early diagnostic and screening applications have been thoroughly analyzed by Qriouet et al [ 14 ] in the review “Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection”. Here, the research team outlined the production process of antibodies against drugs of abuse (such as heroin, amphetamine, benzodiazepines, cannabis, etc.)…”

Recent advances and challenges of biosensing in point-of-care molecular diagnosis

“…LFA is a kind of detection platform based on the movement of liquid samples through a strip-attached molecule which can specifically recognize and interact with the analyte to provide a visible signal [149 ] . Generally, the LFA platform as presented in Fig.…”