Monoclonal antibodies against urokinase

Abstract

Hybridomas secreting antibodies against human urokinase have been produced by the cell-fusion technique (Köhler & Milstein, 1976). They belong to the IgG1 and IgG2 classes. Fixation and inhibition of the binding of 125 I-labelled urokinase, in radioimmunoassay, show that two of the monoclonal antibodies exhibit a high titer in ascitic fluids, a good sensitivity, and no cross reaction with other enzymes showing partial sequence homology with urokinase. Moreover, one of the monoclonal antibodies is able to inhibit the enzymatic activity of urokinase using a chromogenic substrate.

Monoclonal Antibodies Against Human High Molecular Weight Urinary Urokinase: Application for Affinity Purification of Urinary Prourokinase

“…Monoclonal antibodies against human urokinase had been obtained recently also by others (9,10) and had been used for purification of urokinase from partially purified urokinase preparations (10,11), urine (LZ) or human tumor cell line supernatants (13). These antibodies were charact erized regarding their immunoglobulin type (9,10,Lz), urokinase binding titer (9), cross reactivity (9, 10, l2), and functional inhibition of plasminogen activator activity of urokinase (10). None of these antibodies, however, has been used for the purification of prourokinase from urine.…”

A Hybrid Plasminogen Activator Binds to the u-PA Receptor and Has a Reduced Thrombolytic Potency In Vivo

“…All start with the signal peptide (not shaded) and EGF-like domain of urokinase, followed by either the kringle domain of urokinase (Ku) or the second kringle of t-PA (K2) and the protease domain of urokinase (Pr) (15). MAb AAU-2 (25)…”