Abstract:Enzymatic reactions have been widely applied to the design of various sensing systems. However, the detection sensitivity of partial systems is still low, possibly attributed to the large molecular size of the substrate. In this study, we propose a strategy toward sensitive enzyme analysis with monoaromatic substrate. That is, salicyl phosphate ester (SPE) as a fluorimetric probe is presented for evaluating the enzymatic activity of alkaline phosphatase (ALP). The sensing mechanism is that ALP catalyzes the de… Show more
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