Monitoring Wnt/β-Catenin Signaling in Skin

Ku, Amy T.; Miao, Qi; Nguyen, Hoang

doi:10.1007/978-1-4939-6393-5_14

Cited by 9 publications

(7 citation statements)

References 31 publications

(26 reference statements)

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“…Wnt signaling through β-catenin has long been recognized to be crucial for skin epidermal development and homeostasis (32). A number of studies have confirmed that it is essential for cSCC initiation and progression, and for maintaining the cancer stem cell niche (33).…”

Section: Discussionmentioning

confidence: 99%

CDC20 contributes to the development of human cutaneous squamous cell carcinoma through the Wnt/β‑catenin signaling pathway

Chu

Zhang

et al. 2019

Int J Oncol

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Cell division cycle 20 (CDC20) is a regulatory molecule and serves critical roles at multiple points of the cell cycle. Recent evidence indicates that CDC20 may serve an oncogenic role in a number of human cancer types. However, the role of CDC20 in primary cutaneous squamous cell carcinoma (cSCC) has not been studied, to the best of our knowledge. The aim of the present study was to investigate whether and how CDC20 is involved in the tumorigenesis of cSCC. The results revealed that CDC20 expression was significantly increased in cSCC tissues and cell lines, and its expression was associated with pathological differentiation. Downregulation of CDC20 inhibited cell proliferation, induced cell cycle arrest, promoted apoptosis and reduced migratory ability through inhibition of the Wnt/β-catenin signaling pathway. Furthermore, all-trans-retinoic acid treatment significantly downregulated CDC20 expression in cSCC. The present results revealed that CDC20 may serve a crucial role in human cSCC, and suggested that CDC20 may be a novel biomarker for the prevention, diagnosis and treatment of cSCC.

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Section: Discussionmentioning

confidence: 99%

CDC20 contributes to the development of human cutaneous squamous cell carcinoma through the Wnt/β‑catenin signaling pathway

Chu

Zhang

et al. 2019

Int J Oncol

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“…Wnt5a is a member of the wingless-related/mouse mammary tumor virus integration family and can initiate proinflammatory responses through activation of the canonical Wnt/beta-catenin and noncanonical Wnt/Ca(2ϩ) signaling pathways. Wnt5a is restricted primarily to the basal layer in adult skin, with limited expression in the upper spinous and granular layers (34,35). VZV-infected adult skin exhibited increased Wnt5a expression (green signal) adjacent to VZV skin lesions (red signal) in the epidermal compartment (Fig.…”

Section: Resultsmentioning

confidence: 98%

Age-Associated Differences in Infection of Human Skin in the SCID Mouse Model of Varicella-Zoster Virus Pathogenesis

Zerboni

Sung

Lee

et al. 2018

J Virol

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Varicella-zoster virus (VZV) is the skin-tropic human alphaherpesvirus responsible for both varicella-zoster and herpes zoster. Varicella-zoster and herpes zoster skin lesions have similar morphologies, but herpes zoster occurs disproportionally in older individuals and is often associated with a more extensive local rash and severe zoster-related neuralgia. We hypothesized that skin aging could also influence the outcome of the anterograde axonal transport of VZV to skin. We utilized human skin xenografts maintained in immunodeficient (SCID) mice to study VZV-induced skin pathology in fetal and adult skin xenografts. Here we found that VZV replication is enhanced in skin from older compared to younger adults, correlating with clinical observations. In addition to measures of VZV infection, we examined the expression of type I interferon (IFN) pathway components in adult skin and investigated elements of the cutaneous proliferative and inflammatory response to VZV infection Our results demonstrated that VZV infection of adult skin triggers intrinsic IFN-mediated responses such as we have described in VZV-infected fetal skin xenografts, including MxA as well as promyelocytic leukemia protein (PML), in skin cells surrounding lesions. Further, we observed that VZV elicited altered cell signaling and proliferative and inflammatory responses that are involved in wound healing, driven by follicular stem cells. These cellular changes are consistent with VZV-induced activation of STAT3 and suggest that VZV exploits the wound healing process to ensure efficient delivery of the virus to keratinocytes. Adult skin xenografts offer an approach to further investigate VZV-induced skin pathologies Varicella-zoster virus (VZV) is the agent responsible for both varicella-zoster and herpes zoster. Herpes zoster occurs disproportionally in older individuals and is often associated with a more extensive local rash and severe zoster-related neuralgia. To examine the effect of skin aging on VZV skin lesions, we utilized fetal and adult human skin xenografts maintained in immunodeficient (SCID) mice. We measured VZV-induced skin pathology, examined the expression of type I interferon (IFN) pathway components in adult skin, and investigated elements of the cutaneous proliferative and inflammatory response to VZV infection Our results demonstrate that characteristics of aging skin are preserved in xenografts; that VZV replication is enhanced in skin from older compared to younger adults, correlating with clinical observations; and that VZV infection elicits altered cell signaling and inflammatory responses. Adult skin xenografts offer an approach to further investigate VZV-induced skin pathologies .

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“…Wnt signalling plays a critical role in regulating the proliferation, differentiation and motility of skin cells, as well as wound healing and fibrosis 52 . Wnt signalling through β ‐ catenin plays a crucial role in skin development and homeostasis 53 . Several studies have reported dysfunction of the Wnt/β‐catenin signalling pathway in patients with diabetes mellitus wounds and comorbidity in patients with HS 54 …”

Section: Discussionmentioning

confidence: 99%

“…52 Wnt signalling through b-catenin plays a crucial role in skin development and homeostasis. 53 Several studies have reported dysfunction of the Wnt/b-catenin signalling pathway in patients with diabetes mellitus wounds and comorbidity in patients with HS. 54 p53 signalling…”

Section: Wnt/b-catenin Signallingmentioning

confidence: 99%

Methylated miRNAsmay serve as potential biomarkers and therapeutic targets for hidradenitis suppurativa

Radhakrishna

Uppala

Jhala

et al. 2022

Acad Dermatol Venereol

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Background Hidradenitis suppurativa (HS) is a chronic inflammatory disease influenced by genetics, non‐genetic and environmental factors that modulate miRNA expression. Currently, no miRNA data are available for HS. In this study, we profiled DNA methylation patterns of miRNA genes associated with HS susceptibility. Objectives Identify miRNA gene methylation profiles associated with HS susceptibility. This study examined the methylation patterns of DNAs from 24 healthy controls and 24 patients with HS using Illumina Infinium MethylationEPIC BeadChip array analysis. Methylation patterns of miRNA genes were analysed using KEGG pathway analysis to explore the inversely correlated pathways regulated by miRNAs. Results We identified 60 CpG sites representing 65 unique microRNA genes including 54 hypomethylated and 6 hypermethylated CpGs as potentially associated with HS. Some of these CpGs were found to be critical for skin function, such as miR‐29, miR‐200, miR‐205, miR‐548 and miR‐132. The miR‐192 is implicated in non‐alcoholic fatty liver disease. The miR‐200c gene was identified as a vital determinant in regulating skin repair after injury and may contribute to age‐associated alterations in wound repair. miR‐132 was significantly upregulated during the inflammation phase of wound repair, enhancing the activity of STAT3 and ERK pathways that promote keratinocyte proliferation. Conclusions Epigenetically altered microRNA genes are implicated in wound healing, inflammation, keratinocyte proliferation and wound modulation. This is the first study to analyse methylation profiles of miRNA genes in the HS population, highlighting the unique role that miRNAs might play in diagnosing and treating HS.

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Monitoring Wnt/β-Catenin Signaling in Skin

Cited by 9 publications

References 31 publications

CDC20 contributes to the development of human cutaneous squamous cell carcinoma through the Wnt/β‑catenin signaling pathway

CDC20 contributes to the development of human cutaneous squamous cell carcinoma through the Wnt/β‑catenin signaling pathway

Age-Associated Differences in Infection of Human Skin in the SCID Mouse Model of Varicella-Zoster Virus Pathogenesis

Methylated miRNAsmay serve as potential biomarkers and therapeutic targets for hidradenitis suppurativa

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