Methylated miRNAsmay serve as potential biomarkers and therapeutic targets for hidradenitis suppurativa

Abstract

Background Hidradenitis suppurativa (HS) is a chronic inflammatory disease influenced by genetics, non‐genetic and environmental factors that modulate miRNA expression. Currently, no miRNA data are available for HS. In this study, we profiled DNA methylation patterns of miRNA genes associated with HS susceptibility. Objectives Identify miRNA gene methylation profiles associated with HS susceptibility. This study examined the methylation patterns of DNAs from 24 healthy controls and 24 patients with HS using Illumina Infinium MethylationEPIC BeadChip array analysis. Methylation patterns of miRNA genes were analysed using KEGG pathway analysis to explore the inversely correlated pathways regulated by miRNAs. Results We identified 60 CpG sites representing 65 unique microRNA genes including 54 hypomethylated and 6 hypermethylated CpGs as potentially associated with HS. Some of these CpGs were found to be critical for skin function, such as miR‐29, miR‐200, miR‐205, miR‐548 and miR‐132. The miR‐192 is implicated in non‐alcoholic fatty liver disease. The miR‐200c gene was identified as a vital determinant in regulating skin repair after injury and may contribute to age‐associated alterations in wound repair. miR‐132 was significantly upregulated during the inflammation phase of wound repair, enhancing the activity of STAT3 and ERK pathways that promote keratinocyte proliferation. Conclusions Epigenetically altered microRNA genes are implicated in wound healing, inflammation, keratinocyte proliferation and wound modulation. This is the first study to analyse methylation profiles of miRNA genes in the HS population, highlighting the unique role that miRNAs might play in diagnosing and treating HS.

Water Is an Active Element: A Randomized Double-Blind Controlled Clinical Trial Comparing Cutaneous Lipidomics in Consumers Drinking Two Different Bicarbonate-Calcic Waters (Medium-Mineral vs. Oligo-Mineral)

“…Ideally, studies should include the water type orally consumed in the exposome to modify the cutaneous outcomes, especially in complex skin disorders, in which susceptible genes [ 29 , 30 ] interact with exposure to manifest the pathological phenotype (i.e., psoriasis or hidradenitis suppurativa) [ 31 , 32 ]. In fact, exposures may condition the epigenetics that modulate genes expression [ 33 ] and even drug response [ 34 ].…”

Hidradenitis suppurativa associated telomere‐methylome dysregulations in blood

“…A detailed description of the methodology has been previously described. 8 Illumina Infinium methylation EPIC BeadChip Genome-wide DNA methylation analysis was performed on 500 ng of bisulphite-treated genomic DNA using the Illumina The Infinium HumanMethylation450 BeadChip (Illumina Inc., San Diego, CA) according to the manufacturer's protocol 30 at the Beaumont Health Genomics (core facility). All cases and controls, as well as age and gender, were randomly distributed on arrays for successful microarray experiments.…”

miRNA-205: A Future Therapeutic Molecule for Liver Diseases

“…On the other hand, when NZ10 mice were predisposed with obesity, Type 2 diabetes and hepatic steatosis; fed with high protein fish oil diet and examined for the biochemical and physical functions, results showed that the diet prevented steatosis and reduced serum cholesterol and triglycerides, and the diet repressed the hepatic expression of fatty acid metabolic regulators like peroxisome proliferator activated receptor gamma co-activator-1, fatty acid synthase, fatty acid binding protein-4 and apolipoprotein A4 genes in these mice [ 18 ]. To prove miR-205 has the potential to act as a biomarker for other diseases such as Hidradenitis suppurativa, it was found that this, along with other RNAs, could be important for regulating skin and wound repair and age-related alterations [ 21 ].…”