Monitoring the outbreak of equine glanders in Alagoas, Brazil: Clinical, immunological, molecular, and anatomopathological findings

Rocha, Larissa Otaviano da; Lima, Luiz André Rodrigues de; Albuquerque, Rosângela Maria Santos de; Lages, Sonia Luisa Silva; Nunes, Annelise Castanha Barreto Tenório; Castro, Roberto Soares de; Mota, Rinaldo Aparecido; Silva, Karla Patrícia Chaves da; Falcão, Marcus Vinícius Dias

doi:10.1590/0103-8478cr20200834

Cited by 6 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…O aumento relativo de casos, pode ser descrito em decorrência as aglomerações geradas por eventos equestres não fiscalizados pela defesa agropecuária, que invariavelmente reúne animais que não foram testados (Ali-Ani & Roberson, 2007;Verma, 1988). A circulação da mesma cepa da bactéria verificada em estudos realizados por Falcão, et al, (2019) e Rocha et al, (2021) na região Nordeste confirmam a relação existentes no comércio de animais interestadual e aglomerações em eventos e feira à transmissão da doença.…”

Section: Resultsunclassified

Análise espacial do mormo em equídeos no Estado do Tocantins entre 2015 e 2021

Santos

Cardoso²,

Bucar³

et al. 2022

RSD

View full text Add to dashboard Cite

O mormo é uma doença infectocontagiosa, causada pela bactéria Burkholderia mallei que acomete principalmente os equídeos, não havendo tratamento ou vacina. É uma zoonose de relevância econômica, porém com reduzidas publicações científicas sobre sua ocorrência nos estados brasileiros. Assim, o presente trabalho tem por objetivo analisar a distribuição espacial do mormo no estado do Tocantins a partir do geoprocessamento dos focos, bem como a evolução da doença desde o primeiro caso, notificado em 2015, até o ano de 2021. Os dados foram obtidos junto à Agência de Defesa Agropecuária do Tocantins (ADAPEC), e as coordenadas das propriedades focos foram utilizadas para a produção de mapa temático, a partir do programa QGIS, que permitiram a avaliação de distribuição espacial da doença nos anos estudados. O programa foi utilizado para elaboração do mapa de distribuição de rebanho por município organizamos em sistema de escala. No período analisado foram registrados 37 focos e 91 casos de mormo, sendo 74,73% casos na espécie equina, 21,98% de muares e 3,3% de asininos distribuídos de forma não uniforme do Tocantins. Nota-se maior frequência de notificações no norte e no sudoeste do estado, sendo possível evidenciar pontos estratégicos para intensificação de ações de prevenção e controle com o uso do georreferenciamento.

show abstract

Section: Resultsunclassified

Análise espacial do mormo em equídeos no Estado do Tocantins entre 2015 e 2021

Santos

Cardoso²,

Bucar³

et al. 2022

RSD

View full text Add to dashboard Cite

show abstract

“…It has a sensitivity equivalent to or greater than CF, whereas ELISA with recombinant proteins has high sensitivity and specificity (Mota & Ribeiro 2015). Rocha et al (2021) reported that a horse without clinical signs was diagnosed through indirect ELISA; later, the case started to show expectoration of mucopurulent secretion and edema of the hind limb, but even with suggestive signs of glanders and seropositivity in the indirect ELISA, the animal remained negative in the CF.…”

Section: Discussionmentioning

confidence: 99%

Pathology, microbiology, and molecular evaluation of tissues from equids serologically positive for Burkholderia mallei in Midwestern Brazil

Rocha

Oliveira²,

Arruda³

et al. 2023

Pesq. Vet. Bras.

View full text Add to dashboard Cite

Glanders is a disease caused by the bacterium Burkholderia mallei that primarily affects horses, mules and donkeys. The disease can cause lesions in the skin, lungs and several other organs. However, it often manifests as an asymptomatic disease. In Brazil, serological tests of high sensitivity and specificity are used to assist in the detection of antibodies against B. mallei and to contribute to the control of the disease. However, due to the mandatory euthanasia of seroreactive animals, equids with positive serology for B. mallei and asymptomatic generated great conflicts between breeders, veterinarians and diagnostic laboratories. This study clarifies the limitations of complementary diagnostic tests for detecting B. mallei. It describes the clinical, morphological and laboratory findings in 24 equines from different municipalities in the Mato Grosso State, Brazil, which reacted to the complement fixation test and were positive in the western blotting test for glanders. Data and tissue samples were collected from 24 horses for histological, microbiological and molecular analysis. In 23 horses, no clinical signs, morphological alterations, microbiological isolation, or molecular detection would characterize B. mallei infection. On the other hand, samples from an asymptomatic horse without lesional alterations showed sequence amplification compatible with B. mallei in the PCR. Considering that the infection by B. mallei is subject to the application of animal sanitary defense measures and that, by international requirement and national legislation, the serological results are tools that should support the sanitation procedures for the error of the bacteria in the Mato Grosso State, Brazil.

show abstract

“…In Brazil, equids that work in sugar cane fields in the forest zone of the Northeast are more frequently reported to exhibit symptomatic cases of glanders [7][8][9]. Conversely, reports on glanders in other regions generally describe asymptomatic animals or those with mild clinical changes [10,11].…”

Section: Introductionmentioning

confidence: 99%

Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing

Suniga,

Mantovani,

dos Santos

et al. 2023

Pathogens

View full text Add to dashboard Cite

This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders.

show abstract

Monitoring the outbreak of equine glanders in Alagoas, Brazil: Clinical, immunological, molecular, and anatomopathological findings

Cited by 6 publications

References 10 publications

Análise espacial do mormo em equídeos no Estado do Tocantins entre 2015 e 2021

Análise espacial do mormo em equídeos no Estado do Tocantins entre 2015 e 2021

Pathology, microbiology, and molecular evaluation of tissues from equids serologically positive for Burkholderia mallei in Midwestern Brazil

Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing

Contact Info

Product

Resources

About