Monitoring the hemostasis process through the electrical characteristics of a graphene-based field-effect transistor

Schuck, Ariadna; Kim, Hyo Eun; Jung, Kyung‐Mo; Hasenkamp, Willyan; Kim, Yong−Sang

doi:10.1016/j.bios.2020.112167

Cited by 13 publications

(10 citation statements)

References 39 publications

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“…Similarly, antibody-based 2D FET sensors can also detect other proteins such as thrombin, Alzheimer’s disease related tau protein, and prostate specific antigen (PSA), to name a few. However, conventional antibody probes with a molecular weight of greater than 150 kDa are facing bottleneck problems such as relatively high production costs, small amounts of binding epitopes, and slow production rates in expression systems .…”

Section: Biological Sensorsmentioning

confidence: 99%

“…Lab-on-a-chip (LOC) is a device that integrates laboratory functions in a single chip of square millimeters to a few square centimeters . As LOC platforms enable rapid isolation of analytes and require a minimal sample amount, they have attracted increasing attention for analytical applications such as biochemical recognition, human diagnosis, , light detection, , and others. , The fabrication of LOC systems relies on micro–nano manufacturing and differential recognition components according to the analyte. , Among a vast number of LOCs, microfluidic devices are extremely attractive in developing integrated biological sensors because microfluidic technology has enabled precise control of the liquid flow on the sensing surface. ,, Early advances in developing microfluidic-integrated sensing devices focused on photolithography techniques for constructing nanostructures (Figure a), which can be easily conjugated with 2D FET sensors. Additionally, research efforts have been devoted to fabricating microfluidic devices based on a lateral flow (LF) paper stripe .…”

Section: Prototypical Designmentioning

confidence: 99%

“…(d) GFET sensor integrated with optical fibers for multianalyte sensing of light and gas molecules. (a) Adapted with permission from ref . Copyright 2020 Elsevier B.V. (b) Adapted with permission from ref .…”

Section: Prototypical Designmentioning

confidence: 99%

“…16,611 Among a vast number of LOCs, microfluidic devices are extremely attractive in developing integrated biological sensors because microfluidic technology has enabled precise control of the liquid flow on the sensing surface. 254,541,609 Early advances in developing microfluidicintegrated sensing devices focused on photolithography techniques for constructing nanostructures (Figure 32a), which can be easily conjugated with 2D FET sensors. Additionally, research efforts have been devoted to fabricating microfluidic devices based on a lateral flow (LF) paper stripe.…”

Section: Prototypical Designmentioning

confidence: 99%

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Two-Dimensional Field-Effect Transistor Sensors: The Road toward Commercialization

2022

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The evolutionary success in information technology has been sustained by the rapid growth of sensor technology. Recently, advances in sensor technology have promoted the ambitious requirement to build intelligent systems that can be controlled by external stimuli along with independent operation, adaptivity, and low energy expenditure. Among various sensing techniques, field-effect transistors (FETs) with channels made of two-dimensional (2D) materials attract increasing attention for advantages such as label-free detection, fast response, easy operation, and capability of integration. With atomic thickness, 2D materials restrict the carrier flow within the material surface and expose it directly to the external environment, leading to efficient signal acquisition and conversion. This review summarizes the latest advances of 2D-materials-based FET (2D FET) sensors in a comprehensive manner that contains the material, operating principles, fabrication technologies, proof-of-concept applications, and prototypes. First, a brief description of the background and fundamentals is provided. The subsequent contents summarize physical, chemical, and biological 2D FET sensors and their applications. Then, we highlight the challenges of their commercialization and discuss corresponding solution techniques. The following section presents a systematic survey of recent progress in developing commercial prototypes. Lastly, we summarize the long-standing efforts and prospective future development of 2D FET-based sensing systems toward commercialization.

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Section: Biological Sensorsmentioning

confidence: 99%

Section: Prototypical Designmentioning

confidence: 99%

Section: Prototypical Designmentioning

confidence: 99%

Section: Prototypical Designmentioning

confidence: 99%

See 2 more Smart Citations

Two-Dimensional Field-Effect Transistor Sensors: The Road toward Commercialization

2022

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“…These methods characterized by high chemical stability, sensitivity, and specificity have demonstrated sensing benefits. , However, biolabeling and tedious sample pretreatment are often additionally required by these methods, which results in high costs, complex design, and a long-term process. In contrast, label-free methods exhibit unique advantages due to their ability to monitor the binding process of aptamers to analytes in real time. − Because of its excellent sensitivity and selectivity, the solution-gated graphene transistor (SGGT) has been widely developed for biological and chemical sensors in recent years. SGGTs can also work efficiently in electrolytes at a voltage of less than 1 V. − In addition, graphene has high chemical stability in solution, resulting in high stability of SGGTs. , The sensing strategies of SGGTs have attracted significant attention especially in the field of chemical and biological detection and been applied in detection of ions, , proteins, nucleic acid molecules, , and viruses …”

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Solution-Gated Graphene Transistors for Highly Sensitive and Real-Time Detection of Thrombin Molecules

Zhao

Xiao

et al. 2021

Anal. Chem.

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Thrombin is an important biomarker for various diseases and biochemical reactions. Rapid and real-time detection of thrombin that quickly neutralizes in early coagulation in the body has gained significant attention for its practical applications. Solution-gated graphene transistors (SGGTs) have been widely studied due to their higher sensitivity and low-cost fabrication for chemical and biological sensing applications. In this paper, the ssDNA aptamer with 29 bases was immobilized on the surface of the gate electrode to specifically recognize thrombin. The SGGT sensor achieved high sensitivity with a limit of detection (LOD) up to fM. The LOD was attributed to the amplification function of SGGTs and the suitable aptamer choice. The ssDNA configuration folding induced by thrombin molecules and the electropositivity of thrombin molecules could arouse the same electrical response of SGGTs, helping the device obtain a high sensitivity. The channel current variation of sensors had a good linear relationship with the logarithm of thrombin concentration in the range of 1 fM to 10 nM. The fabricated device also demonstrated a short response time to thrombin molecules, and the response time to the 1 fM thrombin molecules was about 150 s. In summary, the sensing strategy of aptamer-based SGGTs with high sensitivity and high selectivity has a good prospect in medical diagnosis.

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Aromatic Ring–Mediated Nonspecific Signaling Mechanism and Nafion‐Dominated Solution in Graphene Field‐Effect Transistor–Based Nucleic Acid Biosensors

Chen

Lyu

Sun

et al. 2023

Adv Funct Materials

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Graphene field‐effect transistors (G‐FETs) have attracted widespread attention in disease diagnosis, benefiting from these advantages of high sensitivity, label‐free, easy integration, and direct detection of nucleic acids (NAs) in liquid environments. However, the problem of nonspecific signals in G‐FETs is not fundamentally solved due to a lack of systematic theoretical research to support the development of effective solutions. Thus, researchers have to rely on speculative mechanisms to minimize nonspecific signals in experiments as much as possible. Herein, the nonspecific signal mechanism caused by eight types of π–π interaction paths mediated by aromatic rings is theoretically determined. Based on theoretical simulation results, the feasibility of blocking nonspecific signal paths through Nafion functionalization methods is experimentally verified. Experiments confirm that Nafion‐modified G‐FETs (NMG‐FETs) have excellent performance in avoiding nonspecific signals compared to traditional G‐FETs. Furthermore, the NMG‐FET achieves ultra‐sensitive detection of Down syndrome–related DNA down to 1 aM and severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) RNA down to 5 aM, and shows good specificity in base recognition. This study is expected to promote the theoretical advancement of the nonspecific signal mechanism in G‐FET NA detection and offer a practical strategy for improving signal purity and accuracy.

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Monitoring the hemostasis process through the electrical characteristics of a graphene-based field-effect transistor

Cited by 13 publications

References 39 publications

Two-Dimensional Field-Effect Transistor Sensors: The Road toward Commercialization

Two-Dimensional Field-Effect Transistor Sensors: The Road toward Commercialization

Aptamer-Based Solution-Gated Graphene Transistors for Highly Sensitive and Real-Time Detection of Thrombin Molecules

Aromatic Ring–Mediated Nonspecific Signaling Mechanism and Nafion‐Dominated Solution in Graphene Field‐Effect Transistor–Based Nucleic Acid Biosensors

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