Monitoring the growth, survival and phenol utilization of the fluorescent-tagged Pseudomonas oleovorans immobilized and free cells

Nandy, Sampurna; Arora, U.; Tarar, Pranay; Viggor, Signe; Jõesaar, Merike; Kivisaar, Maia; Kapley, Atya

doi:10.1016/j.biortech.2021.125568

Cited by 7 publications

(5 citation statements)

References 46 publications

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“…Nandy et al observed that the encapsulated Pseudomonas oleovorans ICTN13 cells stored at 4 °C for 30 days could reduce phenol concentration by 53% in 24 h, retaining half of their catalytic activity, whereas longer storage time (beyond 30 days) reduced phenol removal activity significantly [ 11 ]. Moreover, Banerjee and Ghoshal observed no reduction in phenol degradation efficiency by alginate beads with immobilized Bacillus cereus AKG1 MTCC9817 and AKG2 MTCC 9818 cells upon 30 days of storage [ 56 ].…”

Section: Resultsmentioning

confidence: 99%

“…Cell immobilization prevails over using free bacterial cells for bioremediation applications, solving problems such as substrate inhibition, sensitivity to environmental factors, as well as settling issues with recovery and reusability [ 9 ]. Specifically, the use of gel polymers in bacterial immobilization has proved to be more efficient in terms of phenol toxicity and degradation rates [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

et al. 2023

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Phenol poses a threat as one of the most important industrial environmental pollutants that must be removed before disposal. Biodegradation is a cost-effective and environmentally friendly approach for phenol removal. This work aimed at studying phenol degradation by Pseudarthrobacter phenanthrenivorans Sphe3 cells and also, investigating the pathway used by the bacterium for phenol catabolism. Moreover, alginate-immobilized Sphe3 cells were studied in terms of phenol degradation efficiency compared to free cells. Sphe3 was found to be capable of growing in the presence of phenol as the sole source of carbon and energy, at concentrations up to 1500 mg/L. According to qPCR findings, both pathways of ortho- and meta-cleavage of catechol are active, however, enzymatic assays and intermediate products identification support the predominance of the ortho-metabolic pathway for phenol degradation. Alginate-entrapped Sphe3 cells completely degraded 1000 mg/L phenol after 192 h, even though phenol catabolism proceeds slower in the first 24 h compared to free cells. Immobilized Sphe3 cells retain phenol-degrading capacity even after 30 days of storage and also can be reused for at least five cycles retaining more than 75% of the original phenol-catabolizing capacity.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

et al. 2023

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“…Alginate-biopolymer-immobilized Pseudomonas oleovorans ICTN13 cells showed more than 20% increased efficiency in phenol degradation compared to free cells. Encapsulation of cells increased their life time to 30 days [ 25 ]. The positive effect of immobilization in polyvinyl alcohol–alginate–kaolin beads was also shown in the case of the strain Sphingomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability

Egozarian,

Emelyanova,

Suzina

et al. 2024

Microorganisms

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Biodegradation of phenol is an effective method for removing this toxicant from contaminated sites. Phenol is a toxic compound for living cells, so many bacteria degrade phenol in relatively low concentrations, up to 0.75 g L−1. The Rhodococcus opacus strain 1CP is an effective destructor of a wide range of pollutants. In the absence of a carbon source in the medium, cells of the R. opacus 1CP strain easily form cyst-like resting cells (CLC). The purpose of this work was to evaluate the viability of cells during long-term storage and the efficiency of the process of phenol destruction by R. opacus 1CP cells germinating after dormancy. Resting cells were obtained by simple cultivation in a rich medium followed by storage under static conditions. This is a simple approach to obtain a large amount of biomass. Decomposition of phenol proceeded via catechol followed by ortho-cleavage of aromatic ring. The induction of three phenol hydroxylases was detected by RT-PCR in cells germinated in a mineral medium with phenol as the carbon source. The stability of the genome of cells germinating after dormancy is shown by box-PCR. Dormant R. opacus 1CP cells, both suspended and immobilized, can be directly used for the decomposition of phenol after 4–12 months storage. In addition to phenol, after 9 months of storage, immobilized germinating cells easily metabolized 4-chlorophenol and 2,4,6-trichlorophenol. The results demonstrate a potential and simple approach toward achieving long-term storage of cells for further use in bioremediation.

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“…(Paisio, et al, 2012;Wen, et al, 2020), etc. The biodegradation process is controlled by enzymes (Banerjee and Ghoshal, 2010; Dalvi, et al, 2012;Nandy, et al, 2021). The intermediates produced by phenol biodegradation are catalyzed by the corresponding enzymes to generate pyruvate, acetic acid, succinic acid and acetyl CoA (Loh and Chua, 2002).…”

Section: Introductionmentioning

confidence: 99%

Low-temperature phenol-degrading microbial agent: construction and mechanism

Liu

et al. 2023

Arch Microbiol

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In this study, three cold-tolerant phenol-degrading strains, Pseudomonas veronii Ju-A1 (Ju-A1), Leifsonia naganoensis Ju-A4 (Ju-A4), and Rhodococcus qingshengii Ju-A6 (Ju-A6), were isolated. All three strains can produce cis, cis-muconic acid by ortho-cleavage of catechol at 12 ℃. Response surface methodology (RSM) were used to optimize the proportional composition of low-temperature phenoldegrading microbiota. Degradation of phenol below 160 mg L -1 by low-temperature phenol-degrading microbiota followed rst-order degradation kinetics. When the phenol concentration was greater than 200 mg L -1 , the overall degradation trend was in accordance with the modi ed Gompertz model. The experiments showed that the bacterial agent (three strains of low-temperature phenol-degrading bacteria were fermented separately and constructed in the optimal ratio) degraded phenol at the fastest rate. The above construction method is more advantageous in the actual wastewater treatment.

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Monitoring the growth, survival and phenol utilization of the fluorescent-tagged Pseudomonas oleovorans immobilized and free cells

Cited by 7 publications

References 46 publications

Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

Phenol Degradation by Pseudarthrobacter phenanthrenivorans Sphe3

Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability

Low-temperature phenol-degrading microbial agent: construction and mechanism

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